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Method for preparing fermented virus-free cottonseed meal feed protein

A technology for feed protein and cottonseed meal, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of unsatisfactory detoxification effect of enzymatic hydrolysis method, achieve good detoxification effect, improve nutritional value, increase The effect of protein content

Inactive Publication Date: 2009-06-03
AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological methods include enzymatic hydrolysis and microbial fermentation, but the detoxification effect of enzymatic hydrolysis is not ideal

Method used

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  • Method for preparing fermented virus-free cottonseed meal feed protein
  • Method for preparing fermented virus-free cottonseed meal feed protein
  • Method for preparing fermented virus-free cottonseed meal feed protein

Examples

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Embodiment Construction

[0017] An embodiment of a method for preparing fermented detoxified cottonseed meal feed protein provided by the invention is as follows:

[0018] The strains used in this example are 1 strain of Bacillus subtilis, 1 strain of Lactobacillus, 1 strain of Saccharomyces crevisiae and 1 strain of Candida tropicalis. The specific steps are:

[0019] A. the LB liquid culture medium that is made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 liter of water (pH7.0), pours in the aseptic Erlenmeyer flask, makes Bacillus subtilis seed liquid culture medium and Liquid expansion medium; consisting of 2 grams of peptone, 5 grams of NaCl, 2 grams of glucose, 3 grams of KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4 , 2 grams of starch and 1 liter of water (pH6.5) to form a lactic acid bacteria culture medium, pour it into a sterile triangular flask to make a lactic acid bacteria seed liquid culture medium and a liquid expansion medium; by 100 ...

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Abstract

The invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus ubtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

Description

technical field [0001] The invention relates to a production process of using cottonseed meal as a raw material to transform and produce detoxified cottonseed meal feed protein by using microbial technology, in particular to a microbial fermentation process for preparing detoxified cottonseed meal by multi-bacteria mixed solid fermentation. Background technique [0002] In recent years, my country's animal husbandry and feed industry have developed very rapidly, and the contradiction of shortage of feed resources has become increasingly prominent. According to expert forecasts, from 2010 to 2020, the balance of protein feed in my country will be 24-48 million tons, and the balance of cakes will be 25.6 million tons. For a long time, soybean meal, the protein feed raw material that my country has mainly relied on, has become increasingly tight due to various factors such as output, climate, and domestic and foreign market price fluctuations. The price has risen and fluctuated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14C12N1/20A23K10/12A23K40/00
Inventor 叶明强邝哲师徐志宏赵祥杰罗国庆肖更生潘木水陈薇
Owner AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI
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