Method for preparing fermented virus-free cottonseed meal feed protein
A technology for feed protein and cottonseed meal, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of unsatisfactory detoxification effect of enzymatic hydrolysis method, achieve good detoxification effect, improve nutritional value, increase The effect of protein content
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[0017] An embodiment of a method for preparing fermented detoxified cottonseed meal feed protein provided by the invention is as follows:
[0018] The strains used in this example are 1 strain of Bacillus subtilis, 1 strain of Lactobacillus, 1 strain of Saccharomyces crevisiae and 1 strain of Candida tropicalis. The specific steps are:
[0019] A. the LB liquid culture medium that is made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 liter of water (pH7.0), pours in the aseptic Erlenmeyer flask, makes Bacillus subtilis seed liquid culture medium and Liquid expansion medium; consisting of 2 grams of peptone, 5 grams of NaCl, 2 grams of glucose, 3 grams of KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4 , 2 grams of starch and 1 liter of water (pH6.5) to form a lactic acid bacteria culture medium, pour it into a sterile triangular flask to make a lactic acid bacteria seed liquid culture medium and a liquid expansion medium; by 100 ...
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