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Method for producing bio-protein feedstuff through solid-state fermentation of dreg silybum marianum dreg

A technology of biological protein feed and solid-state fermentation, applied in animal feed, animal feed, application, etc., to achieve the effect of simple process, low cost and short cycle

Active Publication Date: 2013-01-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the application of solid-state fermented silymarin meal to produce protein feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Dry the milk thistle meal and smash it through a sieve. Take 700 grams of milk thistle meal: 200 grams of bran and 100 grams of corn flour to prepare a mixed powder. Based on the dry weight of the above mixed powder, add 5 grams of glucose, 10 grams of urea, and 4 Grams of potassium dihydrogen phosphate are mixed uniformly to make a fermentation medium, the water content is adjusted to 50%, the pH is natural, and it is autoclaved at 121 ℃ for 15 min. Then inoculate a mixed bacterial solution of 5% of the dry weight of the fermentation medium, the mixed bacterial species and the ratio: Aspergillus niger: Candida tropicalis = 1:1, 28 ℃ fermentation for 48 hours, 45 ℃ drying to obtain 935 grams of protein feed. The crude protein content of the protein feed is 37%.

Embodiment 2

[0032] Dry the milk thistle meal and smash it through a sieve. Take 800 g milk thistle meal: 150 g bran: 50 g corn flour to prepare a mixed powder. Based on the dry weight of the above mixed powder, add 10 g glucose, 15 g urea, and 5 Grams of potassium dihydrogen phosphate are mixed uniformly to make a fermentation medium, the water content is adjusted to 60%, the pH is natural, and it is autoclaved at 121 ℃ for 15 min. Then inoculate a mixed bacterial solution of 10% of the dry weight of the fermentation medium, and the mixed bacterial species and ratio: Aspergillus niger: Candida tropicalis=1:1, fermented at 30 ℃ for 87 h, dried at 50 ℃ to obtain 911 grams of protein feed . The crude protein content of the protein feed is 42%.

Embodiment 3

[0034] Dry the milk thistle meal and smash it through a sieve. Take 900 g milk thistle meal: 50 g bran: 50 g corn flour to prepare a mixed powder. Based on the dry weight of the above mixed powder, add 20 g glucose, 20 g urea, and 8 Grams of potassium dihydrogen phosphate are mixed uniformly to make a fermentation medium, the water content is adjusted to 70%, the pH is natural, and it is autoclaved at 121 ℃ for 15 min. Then inoculate a mixture of 15% of the dry weight of the fermentation medium, and the mixed strain and ratio: Aspergillus niger: Candida tropicalis=1:1, fermented at 32 ℃ for 120 h, dried at 65 ℃ to obtain 923 grams of protein feed . The crude protein content of the protein feed is 39%.

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Abstract

The invention relates to a method for producing a bio-protein feedstuff through solid-state fermentation of a dreg which is a silybum marianum dreg. The invention belongs to the technical field of solid-state fermented bio-protein feedstuffs. According to the invention, a mixed microbial agent composed of microbial strains of aspergillus niger and candida tropicalis is adopted. Certain amounts of urea, glucose and potassium dihydrogen phosphate are added into silybum marianum dreg, bran, and corn flour with a certain ratio, and the materials are mixed, such that a solid-state fermentation culture medium is obtained; fermentation is carried out for 48-120h under a temperature of 28-32 DEG C; the material is dried, and a bio-protein feedstuff is obtained. Crude protein content in the protein feedstuff can reach 42%, such that the feedstuff can be applied as an animal culturing protein feedstuff, and can be used for replacing fish meal, meat-bone meal, soybean meal, and the like. The preparation method is simple, and the cost is low. With the method, large-scale productions can be realized. The invention also provides a novel approach for high-efficiency comprehensive utilizations of silybum marianum resources.

Description

technical field [0001] The invention relates to a medicinal dregs biological protein feed and a method thereof, in particular to a biological protein feed produced by solid-state fermentation using medicinal dregs silymarin meal as a raw material and a method thereof, belonging to the technical field of solid-state fermentation biological protein feed. Background technique [0002] In recent years, with the continuous improvement of people's living standards and quality of life, people's demand for the quality of meat, eggs, and milk is increasing. The development of animal husbandry makes the demand for livestock feed more and more, and the requirements are also increasing high. my country's feed industry is in a stage of vigorous development. In 2009, the total amount of feed was about 150 million tons, and it played an increasingly important role in agricultural production and national economy. Protein raw materials in feed production in my country (Yuan Tao, Zhang We...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 仰榴青李峰吴向阳赵婷茆广华郑伟朱洋
Owner JIANGSU UNIV
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