27 results about "Biomin" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a monascus capable of resisting seawater and application of the monascus in high-yield protein. According to the specific technical scheme, the monascus purpureus HXJ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the monascus purpureus HXJ5 is CGMCC No.42056, and the preservation date of the monascus purpureus HXJ5 is July 14, 2025. The content of protein in the monascus purpureus is as high as 37%. And the monascus purpureus can tolerate salt with the concentration of 15%, and can normally grow in a high-salt environment and continuously produce the microbial protein at high yield.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The utility model relates to the technical field of sampling detection, in particular to a sampling device for making biological protein by using residual food on a dining table. The sampling device comprises a sampling machine, a moving frame is fixedly installed on the surface of the sampling machine, a connecting frame is installed in the moving frame in a sliding mode, a containing disc is rotatably installed on the surface of the sampling machine, a sampling pipe is installed in the connecting frame in a sliding mode, one end of the sampling pipe is communicated with a conveying pipe, one end of the conveying pipe is communicated with the moving frame, and the other end of the conveying pipe is communicated with the containing disc. A sample frame is slidably mounted on the surface of the placement disc, a dismounting device is arranged on the arc surface of the connecting frame, the dismounting device comprises two fixing plates, and the two fixing plates are fixedly connected with the arc surface of the connecting frame. The problem that after long-time use, the sampling pipe is damaged or polluted, so that the sampling pipe is difficult to complete the sampling task of the sampling machine or the sampling is unqualified, and the sampling machine cannot be used or the sampling data is deviated is solved.

The present application relates to the technical field of resource recycling, and particularly relates to a kind of biological environment-friendly wastewater protein and its persistent fresh-keeping extraction method.The method is aimed at the problems of high protein content in fish processing wastewater, difficult separation and easy spoilage of recovered products, and proposes a comprehensive process integrating physical strengthening, composite flocculation and biological stabilization, which realizes efficient enrichment and separation of proteins in wastewater without relying on strong chemical conditions.The obtained protein powder has good solubility, structural stability and storage preservation.The process is simple to operate, low in energy consumption and environmentally friendly, and can realize the resource utilization of fish processing wastewater, providing a new technical approach for biological protein extraction and environmental protection treatment.

The utility model relates to the technical field of mixing equipment, and discloses a microbial protein compounding and mixing device which comprises a mixing box, a feed port and a discharge port are respectively formed in the upper part and the lower part of the mixing box, a closable sealing door is mounted at the feed port, a sealing cover is mounted at an opening at the bottom of the discharge port, and the discharge port is communicated with the mixing box. A driving motor is arranged at the top of the mixing box, a main bevel gear is arranged on an output shaft of the driving motor, and a first auxiliary gear and a second auxiliary gear are respectively arranged below and above the main bevel gear. The rotating drum and the rotating shaft can be driven to rotate in opposite directions, so that the rotating shaft can drive the plurality of first stirring assemblies to rotate in a clockwise direction and mix the microbial protein in the mixing box, and the rotating drum can drive the stirring rods to rotate in opposite directions through the plurality of mounting rods and mix the microbial protein.

This invention relates to the field of biological protein extraction technology, specifically disclosing an extraction process for marine biological proteins used for skin whitening in sensitive skin, comprising the following steps: S1, pretreatment; S2, acidic extraction; S3, enzymatic hydrolysis; S4, ultrafiltration and centrifugation. In the acidic extraction process, tannic acid, phytic acid, sulfonated soybean lecithin, and pepsin are added; in the enzymatic hydrolysis process, anthocyanins and magnesium gluconate are added. Through the synergistic effect of these processes, the purity and extraction rate of the marine biological protein are improved.

The invention discloses a method for screening edible and medicinal fungi with high yield of microbial proteins and target active components and a kit thereof, and belongs to the technical field of microbial screening. The method comprises the following steps: carrying out primary culture screening on a culture medium containing a specific precursor; performing safe primary screening; carrying out miniaturized culture and rapid protein detection on the safe strains to evaluate the protein potential, and meanwhile, carrying out visual activity screening by utilizing a report system based on functional characteristics of target active ingredients; and finally, carrying out performance verification on the optimized strain. According to the method disclosed by the invention, high-throughput and synchronous evaluation of the protein yield and the active component synthesis potential of the strain is realized, the bottlenecks of low throughput and single target of a traditional method are solved, the excellent edible and medicinal fungus strain with double effects of one strain can be quickly screened, and a core strain screening tool is provided for efficient production of sustainable protein and natural active components.

The utility model provides a microbial protein solid-liquid separation device, which belongs to the technical field of protein separation equipment and comprises a separation cylinder arranged on a rack, a separation motor arranged at the bottom of the separation cylinder, a screen cylinder arranged in the separation cylinder and rotatably connected with the separation motor, and a cylinder cover detachably arranged at the top of the separation cylinder, a rotating motor is arranged in the barrel cover, the output end of the rotating motor penetrates through the bottom of the barrel cover and is provided with a rotating shaft, a connecting ring is arranged on the surface of the rotating shaft, a linkage rod is fixedly arranged on one side of the connecting ring, and a scraping assembly is arranged at the bottom of the linkage rod. And then a linkage rod connected with a connecting ring is rotated, so that a scraping plate in the scraping assembly is rotated, and attachments on the inner wall of the separation cylinder are rotationally scraped through a cutting edge of the scraping plate, so that the inner wall of the separation cylinder is conveniently cleaned, and the attachments on the inner wall of the separation cylinder are prevented from influencing the subsequent separation quality.

The utility model relates to the technical field of biological protein enzymolysis, and discloses a reaction kettle for biological protein enzymolysis reaction, which comprises a kettle body, a rotating motor is arranged above the kettle body, and a sampling mechanism is arranged in the kettle body; the sampling mechanism comprises a plurality of sampling barrels, each sampling barrel is clamped in the kettle body, a sampling opening is formed in the outer surface of each sampling barrel, and a sliding block is slidably connected into each sampling barrel. According to the reaction kettle for the biological protein enzymolysis reaction, the depths of the plurality of sampling barrels extending into the kettle body are different, and height differences exist among the sampling barrels, so that sampling observation work can be carried out at different heights and positions of the kettle body, and the reaction kettle can be used for better sampling and observing biological protein in enzymolysis; a worker can conveniently and accurately judge the biological protein enzymolysis condition in the reaction kettle, the accuracy of biological protein enzymolysis sampling data is improved, and the use reliability of the biological protein enzymolysis reaction kettle is improved.

The utility model discloses a constant-temperature drying device for biological protein in a solid-phase carrier, and belongs to the technical field of biological protein production. Comprising a support assembly, a drying plate assembly and an adjustable clamp. Wherein the drying plate assembly is fixedly arranged on the upper middle portion of the support assembly, and adjustable clamps which are symmetrically distributed are arranged on the upper surface of the drying plate assembly. The adjustable clamp comprises a lower clamping block and an upper clamping block. The lower clamping block is fixed to the upper surface of the drying plate assembly through glue, the upper surface of the lower clamping block is divided into two areas along the central axis, one area is provided with an anti-skid gasket, and the other area is provided with two screw bases with threads. The adjustable clamp is easy to disassemble and assemble, small in occupied space and convenient to store, the distance between the lower clamping block and the upper clamping block in the adjustable clamp can be adjusted through the bolt, the distance between the adjustable clamps can be adjusted according to the thickness of a solid-phase carrier bearing biological protein, different use requirements are met, and the adjustable clamp is suitable for popularization and application. And the use of the constant-temperature drying device is more diversified.

The invention relates to the technical field of agricultural biology, in particular to application of protein 2370 in degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The invention discloses a gradient density purification membrane and a method for purifying target protein, and relates to the technical field of biological protein purification. The invention comprises a three-layer structure: an outer layer, which has a pore diameter of 1-5 [mu] m and a thickness ratio of 55-65%, and is used as a support layer of a liquid inlet side; the aperture of the middle layer is 0.1-0.3 mu m, the thickness of the middle layer accounts for 25-35%, and the middle layer is formed by staggering nanofibers; the pore diameter of the inner layer is 0.01-0.05 mu m, the thickness of the inner layer accounts for 10-15%, the inner layer serves as a functional layer on the liquid outlet side, and a SpyCatcher ligand is fixed on the surface of the inner layer; the problems that in the prior art, efficiency is low, loading capacity is low, process steps are tedious, and large-scale application of synthetic biology and the like is limited are solved.

The invention relates to the technical field of agricultural biology, in particular to application of a protein 0604 capable of efficiently degrading solanine to degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The utility model provides a biological protein low-temperature drying device. The biological protein low-temperature drying device comprises a machine body, a barrel and a barrel cover. The machine body is provided with an inner cavity, a connecting port is formed in the top of the inner cavity, and a refrigerating pipe and a drying agent are arranged in the inner cavity. The barrel is screwed to the connecting port and used for containing biological protein, and the side wall of the barrel is provided with an air vent capable of being communicated with the inner cavity; the barrel cover is screwed at the top of the barrel body; and the axial length of the air vent is smaller than that of the connecting port. The air vent is blocked at the connecting port along with the screwing-out of the cylinder body, so that the inner cavity of the machine body is separated from the outside, and the low-temperature dry environment in the inner cavity can be kept constant when the biological protein is taken and placed.

The present application relates to the technical field of health food, and particularly relates to a composition capable of improving skin water-retention and water-locking capacity and a preparation method thereof, which comprises the following steps: preparing starch salmon nasal cartilage composite nano powder; preparing flavor moisturizing tremella polysaccharide fermentation liquor; and light enzymolysis of salmon nasal cartilage and compounding with tremella polysaccharide fermentation liquor. The present application mixes a certain proportion of activated and expanded Lactobacillus kefiri subsp. kefiri ZW3 bacterial liquid, Kluyveromyces marxianus bacterial liquid and Bacillus subtilis DE111 bacterial liquid to prepare a composite bacterial liquid, and then ferments the tremella extract, so that the flavor and taste of the tremella extract are improved, the fermentation effect is enhanced, and healthy nutritional ingredients such as microbial proteins, vitamins and amino acids are efficiently generated, the antioxidant capacity is enhanced, and the prepared flavor moisturizing tremella polysaccharide fermentation liquor has rich nutritional ingredients, which can improve the antioxidant capacity and water-retention and moisturizing capacity of human skin when being ingested into the body.

The utility model relates to the field of organic waste treatment, in particular to a system for co-treating organic waste and preparing microbial protein. The system mainly comprises an oxygen supply device, an anaerobic digestion reactor, a microbial protein production unit, an ammonia nitrogen collection unit and a microbial protein collection device. According to the system, methane and hydrogen sulfide in biogas are converted into microbial protein in the microbial protein production unit through MOB and SOB mixed strains, greenhouse gas emission is reduced, and high-added-value products are produced while carbon and sulfur resources are recycled. In addition, the ammonia nitrogen collecting unit effectively recovers nitrogen resources, and meanwhile the environmental emission problem of high-concentration ammonia nitrogen is solved. The stability and efficiency are improved through the synergistic effect of the system components, the resource recovery process is optimized, the economic benefits are increased, and an efficient solution is provided for sustainable waste management and cyclic utilization of carbon, nitrogen and sulfur resources.

The invention relates to the technical field of biomedical engineering and implantable medical instruments, in particular to a biodegradable flexible wireless implantable intracerebral heating device and a preparation method thereof. The device is of a layered structure and sequentially comprises a porous bioprotein substrate layer and a silk protein nanofiber membrane prepared by adopting an electrostatic spinning process from bottom to top, and the crystallinity and degradation rate of the silk protein nanofiber membrane are adjusted through ethanol annealing treatment. And the lower hydrophobic encapsulation layer is positioned on the substrate layer, is made of a biodegradable hydrophobic polymer, preferably polylactic acid (PLA), and is used for isolating biological fluid. And the wireless heating layer is positioned on the lower hydrophobic packaging layer and is formed by depositing a biodegradable metal material, preferably zinc (Zn). And the upper hydrophobic packaging layer covers the wireless heating layer and forms a sealed package for the heating layer together with the lower layer, so that the metal layer is prevented from being corroded by the body fluid too early. The device aims at solving the problems that an existing intracerebral implantation device needs to be taken out through a secondary operation and mechanical mismatch is caused.

The invention discloses a process for producing microbial protein by taking lignocellulose biomass such as corncobs and the like as raw materials, and relates to a method for converting the lignocellulose biomass into the microbial protein by synergistically degrading the lignocellulose biomass through pre-saccharification-mixed bacteria co-fermentation. The method comprises the following specific steps: (1) firstly, pre-saccharifying a lignocellulose biomass raw material by utilizing cellulase; (2) adding filamentous fungi, saccharomycetes, a nitrogen source and other trace elements into the pre-saccharified substrate, and carrying out simultaneous saccharification and mixed bacteria co-fermentation; and (3) carrying out post-treatment on the fermentation product, wherein the post-treatment comprises homogeneous liquid spray drying, centrifugal supernatant spray drying and centrifugal precipitation vacuum drying. The lignocellulose microbial protein product obtained by the method provided by the invention is high in protein and amino acid content and low in cellulose content, and green and efficient conversion from a lignocellulose biomass raw material to a feed protein raw material is promoted.

The invention discloses ancestor laccase and application thereof in detoxification of wheat straw hemicellulose dilute acid hydrolysate, and belongs to the technical field of enzyme engineering. The invention establishes a biological detoxification method based on laccase Anc44 for wheat straw dilute acid hydrolysate containing high-concentration phenolic substances. According to the method, the phenol inhibitor in the hydrolysate can be effectively degraded, so that the growth capability of the candida utilis ATCC22023 and the trichosporon cutaneum ZZ-46 which cannot grow in the high-concentration hydrolysate originally can be recovered. The invention further provides a process for producing the microbial protein by fermenting the detoxified hydrolysate, and the content of bacterial protein produced by fermenting the candida utilis ATCC22023 in the detoxified hydrolysate accounts for 40.282% of that of dry matters. According to the method, a whole-cell catalytic detoxification technology and high-added-value microbial protein production are creatively combined, and the economic benefit and application potential of lignocellulose resource utilization are remarkably improved.

This invention discloses a method for preparing and applying a microbial protease liquid fertilizer, comprising the following steps: S1. Mixing animal and plant protein raw materials with water, and subjecting them to physical-assisted cell wall disruption to obtain a pretreated protein slurry; S2. Inoculating with a composite seed liquid, and performing aerobic fermentation and enzymatic hydrolysis at 45-50℃ for 48-60 hours, maintaining the pH of the fermentation broth at 6.5-7.5 to obtain an enzymatic hydrolysate; S3. Cooling to 28-35℃, inoculating with a mixed probiotic agent, and performing anaerobic or facultative anaerobic fermentation for 48-72 hours to obtain a fermentation broth; S4. Adding trace element salts, and stirring the reaction at 40℃ to 45℃ for 1.5-2.5 hours to allow small molecule active peptides in the fermentation broth to undergo in-situ complexation with metal ions, followed by post-treatment to obtain the microbial protease liquid fertilizer; Through the synergistic effect of physical cell wall disruption and composite enzymatic hydrolysis, the conversion efficiency of recalcitrant waste protein raw materials such as feather meal is significantly improved.

BIOLOGICAL PROTEIN STRUCTURE PREDICTION LABORATORY DEVICE USING GENERATIVE AI

The application belongs to the field of biological fertilizer, and particularly relates to a preparation method of a double biological protein mixture fertilizer and application thereof. The method is prepared by mixing fish and earthworm tissues, and then performing one-step synergistic enzymolysis by using a complex enzyme system containing alkaline protease, flavor protease, chitinase, lipase and trypsin, and then performing enzyme inactivation and filtration. The process can efficiently degrade chitin, and significantly improve the content of small molecule peptides and antioxidant activity in the product. The obtained fertilizer can effectively promote crop growth, enhance stress resistance and improve soil, and can improve potato yield and reduce disease incidence in saline-alkali land planting.

This utility model relates to the field of microbial protein extraction technology, and discloses a solid-liquid separation device for extracting microbial protein from sludge. The device includes a shell, with a rotating disk rotatably connected to the bottom of the shell. Two filter tubes are fixedly connected to the top of the rotating disk. Multiple fixing blocks are fixedly connected to the outside of the two filter tubes. Each of the multiple fixing blocks has a fixing column fixedly connected to each other in pairs. One fixing column has a fixing rod rotatably connected to its outside, and another fixing column has a threaded rod rotatably connected to its outside. In this utility model, loosening the nut allows the threaded rod to rotate, rotating the threaded rod allows the fixing rod to rotate, and rotating the fixing rod loosens the filter screen. Then, pulling the handle removes the filter screen for replacement. Repeating the operation fixes the new filter screen, thus enabling rapid filter screen replacement.

To provide a method for producing protein hydrolysate, and a protein hydrolysate composition.SOLUTION: There is provided a method for producing a protein hydrolysate, comprising the steps of: (a) producing microbial biomass; (b) harvesting the microbial biomass; (c) producing a biomass suspension composition from the harvested microbial biomass; (d) adjusting a pH of the biomass suspension composition to a pH within a first target pH range of at least 11.5, wherein the biomass suspension composition produced in step (c) or the pH adjusted suspension composition produced in step (d) is an alkaline suspension composition; and (e) heating the alkaline suspension composition to a first temperature of at least 110°C for a first time period of at least 5 minutes, thereby producing an alkaline hydrolysate suspension that comprises hydrolyzed microbial protein.SELECTED DRAWING: None

The invention relates to the technical field of exosome preservation, in particular to a method for improving exosome stability, which comprises the following preparation steps: a first preservation solution: adding 15% of ultrafiltration purified human plasma extract, 2% of novel saccharide derivative, 0.8% of functional amino acid combination and 0.05% of specific exosome active substance protective agent into a basic buffer solution; the second preservation solution is prepared by taking a special cell culture medium as a solvent and adding 25% of sheep serum, 4% of polysaccharide substances, 1.5% of trace element mixed solution and 0.1% of antioxidant polypeptide; the third preservation solution is prepared by utilizing a buffer system with specific osmotic pressure and pH value, and adding 30% of pig placenta extract, 6% of cyclodextrin derivative, 2% of protein stabilizer and 0.2% of enzyme activity protective agent aiming at enzymes possibly existing in the exosome; the stability of the exosome under different preservation conditions is effectively improved, and the requirements of the exosome in the fields of biomedical research, clinical diagnosis and treatment and the like are better met.

The invention discloses AI intelligence-based biological protein purification equipment, which comprises a base, a control box and a main box body, the control box is fixedly connected with the top of the base, the outer wall of the control box is fixedly connected with a touch screen, the control box is internally provided with a controller, a memory, a communication module and a power supply, and the top of the base is fixedly connected with a first motor. The output end of the first motor is fixedly connected with the bottom of the main box body, and the bottom of the main box body is fixedly connected with a supporting mechanism. A feeding port is formed in the top of the main box body, a cover plate is slidably inserted into the feeding port, one end of the cover plate penetrates through the inner wall of the feeding port and extends outwards, a locking mechanism corresponding to the cover plate is arranged on the outer wall of the main box body, a discharging pipe is fixedly inserted into the bottom of the inner wall of one side of the main box body, and a discharging valve is connected to the discharging pipe in a threaded mode. Through the arrangement of the monitoring control mechanism, the purification equipment can be remotely and automatically controlled, so that the use effect of the biological protein purification equipment is effectively improved.