The present invention relates to a high-activity microbial protein forage and a preparation method thereof. The microbial high-activity preparation comprises the following components: 10%-20% of photosynthetic bacteria, 20%-40% of lactobacilli, 10%-20% of Saccharomyces cerevisiae Hansen, 20%-30% of bacillus subtilis, and 10%-20% of bifidobacteria. The microbial protein forage comprises the following components: dried fish, blood powder, soybean, soybean meal, peanut meal, DDGS forage, calcium hydrogen phosphate, salt, soybean oligosaccharides, microbial high-activity preparation and vitamin composite premix. In preparation, high-temperature puffing and stirring are performed on the dried fish, blood powder and soybean. The components such as soybean meal, peanut meal and DDGS forage are added. The microbial high-activity preparation is sprayed for being added, and then vacuum packaging is performed. The microbial preparation can also be adopted for preparing mixed forage for grices. The microbial high-activity preparation of the invention can be fed directly and has high feeding safety. The microbial high-activity preparation can be widely used for ecological cultivation of livestock, poultry and aquatic product. The high-activity microbial protein forage has the following advantages: improving forage conversion rate of the pig, preventing disease and treating disease, improving survival rate, purifying environment, reducing breeding cost, obtaining obvious yield and income increasing effect, and obtaining good popularization value.