47 results about "Biomin" patented technology

The invention relates to the technical field of health-care food, in particular to a composition capable of improving water retention and water locking capacity of skin and a preparation method of the composition. Comprising the following steps: preparing starch salmon nasal cartilage composite nano powder; preparing flavor moisturizing tremella polysaccharide fermentation liquor; and light enzymolysis of the salmon nasal cartilage and compounding of the salmon nasal cartilage and the tremella polysaccharide fermentation liquor. According to the invention, the lactobacillus kefiranofaciens kluyveromyces marxianus subsp. Kefiranofaciens ZW3 bacterial liquid, the kluyveromyces marxianus bacterial liquid and the bacillus subtilis DE111 bacterial liquid which are activated and propagated according to a certain proportion are mixed to prepare the compound bacterial liquid, and then the tremella fuciformis extracting solution is fermented, so that the flavor and taste of the tremella fuciformis extracting solution can be improved, the fermentation effect can be enhanced, and the quality of the tremella fuciformis is improved. Healthy nutritional ingredients such as microbial protein, vitamins and amino acid can be efficiently generated, the antioxidant capacity is enhanced, and the prepared flavored moisturizing tremella polysaccharide fermentation liquor is rich in nutritional ingredients and can improve the antioxidant capacity and the water retention and moisturizing capacity of human skin after being taken into a human body.

The invention belongs to the technical field of microbial protein preparation, and particularly relates to a method for preparing microbial protein from crop straw and application of the microbial protein. According to the method, the crop straw is subjected to biomass conversion pretreatment, so that the accessibility and hydrolysis efficiency of cellulose are improved; on the basis, the pretreated biomass is subjected to enzymolysis through cellulase, cellulose and hemicellulose components in the biomass are converted into glucose and xylose, and the glucose and the xylose are utilized by saccharomycetes in the subsequent saccharomycetes fermentation process to synthesize the microbial protein. The microbial protein obtained through the method has the advantages of being rich in nutrition and high in essential amino acid content, and has wide application prospects in the fields of food and feed. Therefore, after the crop straw is subjected to chemical or physical-biological treatment, a new way is provided for high-value utilization of the crop straw on the basis of obtaining the nutrient-rich microbial protein.

The invention belongs to the technical field of microorganisms, and particularly relates to a monascus capable of resisting seawater and application of the monascus in high-yield protein. According to the specific technical scheme, the monascus purpureus HXJ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the monascus purpureus HXJ5 is CGMCC No.42056, and the preservation date of the monascus purpureus HXJ5 is July 14, 2025. The content of protein in the monascus purpureus is as high as 37%. And the monascus purpureus can tolerate salt with the concentration of 15%, and can normally grow in a high-salt environment and continuously produce the microbial protein at high yield.

The invention belongs to the technical field of microbial protein production, and particularly relates to a method for improving the yield of microbial protein functional bacteria. The method comprises the following steps that enriched nitrogen-fixing gas hydrogen-oxidizing bacteria and an ammonium-free culture medium are mixed and then inoculated into a bioreactor, a gas supply strategy is dynamically adjusted in a multi-stage mode according to biomass changes, the biomass is expressed by OD600, and the method specifically comprises the following processes that when OD600 is smaller than a preset value, a first-stage gas supply method is adopted; when OD600 changes to a preset value, the gas supply mode is adjusted from the first stage to the next stage; the proportion of oxygen in the gas supply method in the first stage is lower than that in the gas supply method in the later stage. When the biomass is low, inhibition of oxygen on nitrogenase is relieved by properly reducing the proportion of oxygen, the delay period of the earlier culture period is shortened, oxygen is supplemented in time after the biomass is increased, sufficient electron and energy supply is guaranteed, high-speed growth in the rapid growth period is maintained, and efficient growth of nitrogen-fixing hydrogen oxidizing bacteria is achieved.

The present invention relates to the field of food processing technology, and in particular to a semi-fermented microbial protein meat product and a preparation method thereof. The preparation method comprises: mixing yeast protein and soy protein in a mass ratio of (2-5): (5-8) and then extruding through high moisture content to form a fibrous protein; the temperature program of the high moisture content extrusion comprises: (55-65°C)-(75-85°C)-(133-137°C)-(143-147°C)-(117-123°C); fermenting, frying and drying the fibrous protein. The present invention uses a specific ratio of yeast protein and soy protein to prepare a fibrous protein through high moisture content protein extrusion, which has a distinct fiber structure. The microbial protein meat product prepared after combining subsequent fermentation processes has both the advantages of microbial fermented food and better flavor and sensory experience.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The invention belongs to the technical field of animal nutrition, and particularly relates to a biological protein and a preparation method and application thereof. The biological protein is mainly prepared from the following raw materials in percentage by weight through fermentation: 83 to 87 weight percent of corn protein, 6.5 to 7.5 weight percent of nostoc sphaeroides, 4 to 8 weight percent of sea coconut and 2.5 to 3.5 weight percent of glucose and a microbial agent; the microbial agent is lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis; in the biological protein, the content of crude protein is 49 to 54 weight percent, the content of true protein is 42 to 48 weight percent, the content of crude fiber is 4.5 to 5.6 weight percent, and the content of total acid is 8.2 to 8.9 weight percent; the viable count is 4.5-5.5 * 10 < 9 > cfu / mL, and the protein solubility is 73-79 wt%. The biological protein produced by the invention has higher protein solubility and beneficial viable bacteria, and these properties have important meanings for improving nutrient absorption and health conditions of animals such as livestock (such as dairy cows), poultry and the like, and also can improve the laying rate of the poultry and the milk production rate of the livestock and dairy cows, so that the production cost is reduced, and the economic benefit is increased. The safety and the health degree of the food are improved, and the method has a relatively good commercial application prospect.

The utility model relates to the technical field of sampling detection, in particular to a sampling device for making biological protein by using residual food on a dining table. The sampling device comprises a sampling machine, a moving frame is fixedly installed on the surface of the sampling machine, a connecting frame is installed in the moving frame in a sliding mode, a containing disc is rotatably installed on the surface of the sampling machine, a sampling pipe is installed in the connecting frame in a sliding mode, one end of the sampling pipe is communicated with a conveying pipe, one end of the conveying pipe is communicated with the moving frame, and the other end of the conveying pipe is communicated with the containing disc. A sample frame is slidably mounted on the surface of the placement disc, a dismounting device is arranged on the arc surface of the connecting frame, the dismounting device comprises two fixing plates, and the two fixing plates are fixedly connected with the arc surface of the connecting frame. The problem that after long-time use, the sampling pipe is damaged or polluted, so that the sampling pipe is difficult to complete the sampling task of the sampling machine or the sampling is unqualified, and the sampling machine cannot be used or the sampling data is deviated is solved.

The present application relates to the technical field of resource recycling, and particularly relates to a kind of biological environment-friendly wastewater protein and its persistent fresh-keeping extraction method.The method is aimed at the problems of high protein content in fish processing wastewater, difficult separation and easy spoilage of recovered products, and proposes a comprehensive process integrating physical strengthening, composite flocculation and biological stabilization, which realizes efficient enrichment and separation of proteins in wastewater without relying on strong chemical conditions.The obtained protein powder has good solubility, structural stability and storage preservation.The process is simple to operate, low in energy consumption and environmentally friendly, and can realize the resource utilization of fish processing wastewater, providing a new technical approach for biological protein extraction and environmental protection treatment.

The utility model relates to the technical field of mixing equipment, and discloses a microbial protein compounding and mixing device which comprises a mixing box, a feed port and a discharge port are respectively formed in the upper part and the lower part of the mixing box, a closable sealing door is mounted at the feed port, a sealing cover is mounted at an opening at the bottom of the discharge port, and the discharge port is communicated with the mixing box. A driving motor is arranged at the top of the mixing box, a main bevel gear is arranged on an output shaft of the driving motor, and a first auxiliary gear and a second auxiliary gear are respectively arranged below and above the main bevel gear. The rotating drum and the rotating shaft can be driven to rotate in opposite directions, so that the rotating shaft can drive the plurality of first stirring assemblies to rotate in a clockwise direction and mix the microbial protein in the mixing box, and the rotating drum can drive the stirring rods to rotate in opposite directions through the plurality of mounting rods and mix the microbial protein.

This invention relates to the field of biological protein extraction technology, specifically disclosing an extraction process for marine biological proteins used for skin whitening in sensitive skin, comprising the following steps: S1, pretreatment; S2, acidic extraction; S3, enzymatic hydrolysis; S4, ultrafiltration and centrifugation. In the acidic extraction process, tannic acid, phytic acid, sulfonated soybean lecithin, and pepsin are added; in the enzymatic hydrolysis process, anthocyanins and magnesium gluconate are added. Through the synergistic effect of these processes, the purity and extraction rate of the marine biological protein are improved.

The invention discloses a method for screening edible and medicinal fungi with high yield of microbial proteins and target active components and a kit thereof, and belongs to the technical field of microbial screening. The method comprises the following steps: carrying out primary culture screening on a culture medium containing a specific precursor; performing safe primary screening; carrying out miniaturized culture and rapid protein detection on the safe strains to evaluate the protein potential, and meanwhile, carrying out visual activity screening by utilizing a report system based on functional characteristics of target active ingredients; and finally, carrying out performance verification on the optimized strain. According to the method disclosed by the invention, high-throughput and synchronous evaluation of the protein yield and the active component synthesis potential of the strain is realized, the bottlenecks of low throughput and single target of a traditional method are solved, the excellent edible and medicinal fungus strain with double effects of one strain can be quickly screened, and a core strain screening tool is provided for efficient production of sustainable protein and natural active components.

The utility model provides a microbial protein solid-liquid separation device, which belongs to the technical field of protein separation equipment and comprises a separation cylinder arranged on a rack, a separation motor arranged at the bottom of the separation cylinder, a screen cylinder arranged in the separation cylinder and rotatably connected with the separation motor, and a cylinder cover detachably arranged at the top of the separation cylinder, a rotating motor is arranged in the barrel cover, the output end of the rotating motor penetrates through the bottom of the barrel cover and is provided with a rotating shaft, a connecting ring is arranged on the surface of the rotating shaft, a linkage rod is fixedly arranged on one side of the connecting ring, and a scraping assembly is arranged at the bottom of the linkage rod. And then a linkage rod connected with a connecting ring is rotated, so that a scraping plate in the scraping assembly is rotated, and attachments on the inner wall of the separation cylinder are rotationally scraped through a cutting edge of the scraping plate, so that the inner wall of the separation cylinder is conveniently cleaned, and the attachments on the inner wall of the separation cylinder are prevented from influencing the subsequent separation quality.

The utility model relates to the technical field of biological protein enzymolysis, and discloses a reaction kettle for biological protein enzymolysis reaction, which comprises a kettle body, a rotating motor is arranged above the kettle body, and a sampling mechanism is arranged in the kettle body; the sampling mechanism comprises a plurality of sampling barrels, each sampling barrel is clamped in the kettle body, a sampling opening is formed in the outer surface of each sampling barrel, and a sliding block is slidably connected into each sampling barrel. According to the reaction kettle for the biological protein enzymolysis reaction, the depths of the plurality of sampling barrels extending into the kettle body are different, and height differences exist among the sampling barrels, so that sampling observation work can be carried out at different heights and positions of the kettle body, and the reaction kettle can be used for better sampling and observing biological protein in enzymolysis; a worker can conveniently and accurately judge the biological protein enzymolysis condition in the reaction kettle, the accuracy of biological protein enzymolysis sampling data is improved, and the use reliability of the biological protein enzymolysis reaction kettle is improved.

The utility model discloses a constant-temperature drying device for biological protein in a solid-phase carrier, and belongs to the technical field of biological protein production. Comprising a support assembly, a drying plate assembly and an adjustable clamp. Wherein the drying plate assembly is fixedly arranged on the upper middle portion of the support assembly, and adjustable clamps which are symmetrically distributed are arranged on the upper surface of the drying plate assembly. The adjustable clamp comprises a lower clamping block and an upper clamping block. The lower clamping block is fixed to the upper surface of the drying plate assembly through glue, the upper surface of the lower clamping block is divided into two areas along the central axis, one area is provided with an anti-skid gasket, and the other area is provided with two screw bases with threads. The adjustable clamp is easy to disassemble and assemble, small in occupied space and convenient to store, the distance between the lower clamping block and the upper clamping block in the adjustable clamp can be adjusted through the bolt, the distance between the adjustable clamps can be adjusted according to the thickness of a solid-phase carrier bearing biological protein, different use requirements are met, and the adjustable clamp is suitable for popularization and application. And the use of the constant-temperature drying device is more diversified.

The utility model relates to the technical field of injectors, and discloses an injector for injection of anti-HPV biological protein dressing, which comprises a tube body, one end of the surface of the tube body is in threaded connection with a protective cover, one end of the tube body is provided with an injection hole, the inner wall of the tube body is in sliding connection with a push rod, one end of the push rod is fixedly connected with a piston, and the surface of the tube body is provided with round holes. A limiting ring and a recycling pipe are fixedly connected to the surface of the pipe body, a recycling groove and a discharging hole are formed in the surface of the recycling pipe, a shell is fixedly connected to one side of the limiting ring, connecting holes matched with the recycling groove and the discharging hole are formed in the surface of the shell, and an adjusting ring is rotationally connected between the shell and the recycling pipe. Via holes are formed in the surfaces of the adjusting rings, and limiting mechanisms are fixedly installed on the surface of the shell. According to the injector for injection of the anti-HPV biological protein dressing, the anti-HPV biological protein dressing can be conveniently and evenly smeared on the inner wall of the vagina, meanwhile, secretions in the vagina can be cleaned, and the drug effect of the anti-HPV biological protein dressing is improved.

The invention relates to the technical field of agricultural biology, in particular to application of protein 2370 in degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The invention discloses a gradient density purification membrane and a method for purifying target protein, and relates to the technical field of biological protein purification. The invention comprises a three-layer structure: an outer layer, which has a pore diameter of 1-5 [mu] m and a thickness ratio of 55-65%, and is used as a support layer of a liquid inlet side; the aperture of the middle layer is 0.1-0.3 mu m, the thickness of the middle layer accounts for 25-35%, and the middle layer is formed by staggering nanofibers; the pore diameter of the inner layer is 0.01-0.05 mu m, the thickness of the inner layer accounts for 10-15%, the inner layer serves as a functional layer on the liquid outlet side, and a SpyCatcher ligand is fixed on the surface of the inner layer; the problems that in the prior art, efficiency is low, loading capacity is low, process steps are tedious, and large-scale application of synthetic biology and the like is limited are solved.

The invention relates to the technical field of agricultural biology, in particular to application of a protein 0604 capable of efficiently degrading solanine to degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The utility model provides a biological protein low-temperature drying device. The biological protein low-temperature drying device comprises a machine body, a barrel and a barrel cover. The machine body is provided with an inner cavity, a connecting port is formed in the top of the inner cavity, and a refrigerating pipe and a drying agent are arranged in the inner cavity. The barrel is screwed to the connecting port and used for containing biological protein, and the side wall of the barrel is provided with an air vent capable of being communicated with the inner cavity; the barrel cover is screwed at the top of the barrel body; and the axial length of the air vent is smaller than that of the connecting port. The air vent is blocked at the connecting port along with the screwing-out of the cylinder body, so that the inner cavity of the machine body is separated from the outside, and the low-temperature dry environment in the inner cavity can be kept constant when the biological protein is taken and placed.

The present application relates to the technical field of health food, and particularly relates to a composition capable of improving skin water-retention and water-locking capacity and a preparation method thereof, which comprises the following steps: preparing starch salmon nasal cartilage composite nano powder; preparing flavor moisturizing tremella polysaccharide fermentation liquor; and light enzymolysis of salmon nasal cartilage and compounding with tremella polysaccharide fermentation liquor. The present application mixes a certain proportion of activated and expanded Lactobacillus kefiri subsp. kefiri ZW3 bacterial liquid, Kluyveromyces marxianus bacterial liquid and Bacillus subtilis DE111 bacterial liquid to prepare a composite bacterial liquid, and then ferments the tremella extract, so that the flavor and taste of the tremella extract are improved, the fermentation effect is enhanced, and healthy nutritional ingredients such as microbial proteins, vitamins and amino acids are efficiently generated, the antioxidant capacity is enhanced, and the prepared flavor moisturizing tremella polysaccharide fermentation liquor has rich nutritional ingredients, which can improve the antioxidant capacity and water-retention and moisturizing capacity of human skin when being ingested into the body.

The invention discloses a preparation method and application of a cell lysate gel for treating oral ulcers, and relates to the technical field of oral care. The cell lysate gel is prepared by mixing a functional solution and thrombin, the functional solution comprises a solvent and a solute, the solvent is a mesenchymal stem cell lysate, the solute comprises bovine fibrinogen, borneol, vitamins, polysaccharides, an immunopotentiator, chlorogenic acid, indomethacin, and ginkgo biloba, the vitamins comprise vitamin E and vitamin B2, the polysaccharides comprise astragalus polysaccharides and cuttlebone polysaccharides, the immunopotentiator comprises alpha-mannan peptide and total glucosides of white paeony. The invention avoids the side effects of mesenchymal stem cells, has the advantages of high fluidity, simple and convenient preparation, safety and environmental protection, good application treatment effect, and the like, and achieves remarkable results in the aspects of oral ulcer anti-inflammatory effect, oral ulcer angiogenesis, and enhancing rat immunity, thereby reflecting the great prospect of oral ulcer treatment.

The utility model relates to the field of organic waste treatment, in particular to a system for co-treating organic waste and preparing microbial protein. The system mainly comprises an oxygen supply device, an anaerobic digestion reactor, a microbial protein production unit, an ammonia nitrogen collection unit and a microbial protein collection device. According to the system, methane and hydrogen sulfide in biogas are converted into microbial protein in the microbial protein production unit through MOB and SOB mixed strains, greenhouse gas emission is reduced, and high-added-value products are produced while carbon and sulfur resources are recycled. In addition, the ammonia nitrogen collecting unit effectively recovers nitrogen resources, and meanwhile the environmental emission problem of high-concentration ammonia nitrogen is solved. The stability and efficiency are improved through the synergistic effect of the system components, the resource recovery process is optimized, the economic benefits are increased, and an efficient solution is provided for sustainable waste management and cyclic utilization of carbon, nitrogen and sulfur resources.

The invention relates to the technical field of biomedical engineering and implantable medical instruments, in particular to a biodegradable flexible wireless implantable intracerebral heating device and a preparation method thereof. The device is of a layered structure and sequentially comprises a porous bioprotein substrate layer and a silk protein nanofiber membrane prepared by adopting an electrostatic spinning process from bottom to top, and the crystallinity and degradation rate of the silk protein nanofiber membrane are adjusted through ethanol annealing treatment. And the lower hydrophobic encapsulation layer is positioned on the substrate layer, is made of a biodegradable hydrophobic polymer, preferably polylactic acid (PLA), and is used for isolating biological fluid. And the wireless heating layer is positioned on the lower hydrophobic packaging layer and is formed by depositing a biodegradable metal material, preferably zinc (Zn). And the upper hydrophobic packaging layer covers the wireless heating layer and forms a sealed package for the heating layer together with the lower layer, so that the metal layer is prevented from being corroded by the body fluid too early. The device aims at solving the problems that an existing intracerebral implantation device needs to be taken out through a secondary operation and mechanical mismatch is caused.

The invention discloses a process for producing microbial protein by taking lignocellulose biomass such as corncobs and the like as raw materials, and relates to a method for converting the lignocellulose biomass into the microbial protein by synergistically degrading the lignocellulose biomass through pre-saccharification-mixed bacteria co-fermentation. The method comprises the following specific steps: (1) firstly, pre-saccharifying a lignocellulose biomass raw material by utilizing cellulase; (2) adding filamentous fungi, saccharomycetes, a nitrogen source and other trace elements into the pre-saccharified substrate, and carrying out simultaneous saccharification and mixed bacteria co-fermentation; and (3) carrying out post-treatment on the fermentation product, wherein the post-treatment comprises homogeneous liquid spray drying, centrifugal supernatant spray drying and centrifugal precipitation vacuum drying. The lignocellulose microbial protein product obtained by the method provided by the invention is high in protein and amino acid content and low in cellulose content, and green and efficient conversion from a lignocellulose biomass raw material to a feed protein raw material is promoted.

The present invention relates to a silk fibroin biomaterial with a high β-folding content and a preparation method thereof, and belongs to the field of biological extraction technology. The present invention first prepares a regenerated silk fibroin solution; an enzyme catalytic auxiliary and an enzyme are added to the obtained regenerated silk fibroin solution for incubation; the enzyme catalytic auxiliary is selected from one or more of a metal ion catalyst, a zwitterionic surfactant, a functionalized ionic liquid and a calcium phosphate nanocluster; the resulting product is centrifuged, cleaned and dried to obtain a silk fibroin biomaterial with a high β-folding content. The silk fibroin material of the present invention has a higher β-folding amount through the modification of the primary structure, the spatial structure is more crystallized, and the amino acid sequence is more regular and orderly. The silk fibroin material of the present invention has excellent mechanical properties, efficiency and yield, and is a biological protein material with great development potential.

The invention discloses ancestor laccase and application thereof in detoxification of wheat straw hemicellulose dilute acid hydrolysate, and belongs to the technical field of enzyme engineering. The invention establishes a biological detoxification method based on laccase Anc44 for wheat straw dilute acid hydrolysate containing high-concentration phenolic substances. According to the method, the phenol inhibitor in the hydrolysate can be effectively degraded, so that the growth capability of the candida utilis ATCC22023 and the trichosporon cutaneum ZZ-46 which cannot grow in the high-concentration hydrolysate originally can be recovered. The invention further provides a process for producing the microbial protein by fermenting the detoxified hydrolysate, and the content of bacterial protein produced by fermenting the candida utilis ATCC22023 in the detoxified hydrolysate accounts for 40.282% of that of dry matters. According to the method, a whole-cell catalytic detoxification technology and high-added-value microbial protein production are creatively combined, and the economic benefit and application potential of lignocellulose resource utilization are remarkably improved.

The present invention belongs to the field of microorganisms, and particularly relates to a strain of Xanthobacter flavus and a method for synthesizing biological protein using the same. The specific technical solution is as follows: a strain of Xanthobacter flavus CIB1, which was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on May 19, 2022, with the deposit number: CGMCC NO. 24923. The present invention provides a method for a hydrogen-oxidizing bacterium to synthesize biological protein using CO2 and air. Under the condition of no addition of nitrogen source compounds, it can not only achieve the capture and reduction of CO2, but also obtain high-value-added biological protein, having the advantages of low cost and environmental friendliness.

The utility model relates to the technical field of bioprotein production, and particularly discloses a bioprotein low-temperature drying device which comprises a box body and a storage mechanism, the storage mechanism comprises a box cover, a placing frame, two mounting plates, a lifting plate, a motor, a screw rod, two groups of opening and closing assemblies, a guide assembly and a regulation and control assembly, and each group of opening and closing assembly comprises an abutting frame, a supporting rod and a round rod. The lifting plate is fixedly connected to one side of the containing frame, the lead screw penetrates through the lifting plate and is in threaded fit with the lifting plate, the two ends of the lead screw are rotationally connected with the two mounting plates correspondingly, the output end of the motor is fixedly connected with the lead screw, the abutting frame is fixedly connected with the box cover and located on the bottom face of the box cover, and the supporting rod is fixedly connected to the upper surface of the containing frame. One end of the round rod is fixedly connected with the supporting rod, the other end of the round rod penetrates through the abutting frame and is in sliding connection with the abutting frame, and through the arrangement, the storage and taking efficiency of the biological protein is improved.

This invention discloses a method for preparing and applying a microbial protease liquid fertilizer, comprising the following steps: S1. Mixing animal and plant protein raw materials with water, and subjecting them to physical-assisted cell wall disruption to obtain a pretreated protein slurry; S2. Inoculating with a composite seed liquid, and performing aerobic fermentation and enzymatic hydrolysis at 45-50℃ for 48-60 hours, maintaining the pH of the fermentation broth at 6.5-7.5 to obtain an enzymatic hydrolysate; S3. Cooling to 28-35℃, inoculating with a mixed probiotic agent, and performing anaerobic or facultative anaerobic fermentation for 48-72 hours to obtain a fermentation broth; S4. Adding trace element salts, and stirring the reaction at 40℃ to 45℃ for 1.5-2.5 hours to allow small molecule active peptides in the fermentation broth to undergo in-situ complexation with metal ions, followed by post-treatment to obtain the microbial protease liquid fertilizer; Through the synergistic effect of physical cell wall disruption and composite enzymatic hydrolysis, the conversion efficiency of recalcitrant waste protein raw materials such as feather meal is significantly improved.