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Method for producing protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and protein feed being produced via method for producing protein feed through hypsizygus marmoreus fungus chaff solid state fermentation

A protein feed and solid-state fermentation technology, applied in animal feed, animal feed, application and other directions, can solve the problems such as the unseen problems of shiitake mushroom bran protein feed, and achieve the effect of realizing waste utilization, reducing feeding cost and enriching nutrition.

Active Publication Date: 2013-03-20
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the existing fungus chaff feed is made by mixing waste materials, cottonseed, bran, corn flour, etc., and some fungus chaff feed is made by fermenting EM bacteria agent, etc. , using a microbial mixture, that is, a mixture of Candida tropicalis, Candida utilis and Geotrichum candidum, the fermented jitake mushroom fur protein feed has not yet been seen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Preparation of bacteria agent

[0013] 1.1 Preparation of M. utilis [Candida utilis (Henneberg) Lodder & Kreger-van Rij]:

[0014] Culture medium: bean sprout juice liquid medium. Wash 200g of soybean sprouts or mung bean sprouts, boil in 1000mL for 30min, filter through 4 layers of gauze to obtain bean sprouts juice, add (NH 4 ) 2 HPO 4 1g, KCl0.2g, MgSO 4 ·7H 2 O0.2g, glucose 20g, added H 2 0 to 1000mL, with natural pH, divided into Erlenmeyer flasks, and sterilized at 121°C for 20min.

[0015] A. utilis [purchased from the Culture Collection Center of Guangdong Institute of Microbiology (GIMCC), No. ACCC20060] was inoculated in the culture medium, and cultured on a temperature-controlled shaker at 25°C and 150 rpm for 48 hours. The measured OD value of the bacterial solution ranges from 0.1 to 1.2, that is, 6×10 per milliliter 6 ~5×10 7 A Trichomonas utilis, stop fermentation, obtain the fermented liquid of Trichosanthes utilis thus.

[0016] 1.2 Prepara...

Embodiment 2

[0028] 1. The preparation of bacterial agent is the same as that in Example 1, thus respectively obtaining the fermented liquid of A. utilis, Candida tropicalis and Geotrichum candidum.

[0029] 2. Preparation of solid culture medium of jiji mushroom chaff

[0030] The solid medium of jitake mushroom chaff includes solid material and water. The solid material is mainly made of jitake mushroom chaff, supplemented with bran and urea, etc. The solid material is calculated as 100% by mass fraction, including 90%, bran 9%, urea 0.5%, KH 2 PO 4 0.5%, mix the components together and stir evenly, then add water, the mass ratio of material to water is 1:1.5, then stir evenly, the initial pH value is 5.5, sterilize at 121°C for 40min, and set aside.

[0031] 3. Solid fermentation

[0032]Inoculate A. utilis in the solid culture medium of jitake mushroom chaff, and inoculate 15ml of fermented liquid of A. utilis in every kilogram of jitake mushroom chaff solid medium, about 9×10 7 ~7...

Embodiment 3

[0035] 1. The preparation of bacterial agent is the same as that in Example 1, thus respectively obtaining the fermented liquid of A. utilis, Candida tropicalis and Geotrichum candidum.

[0036] 2. Preparation of solid culture medium of jiji mushroom chaff

[0037] The solid medium of jitake mushroom chaff includes solid material and water. The solid material is mainly made of jitake mushroom chaff, supplemented with bran and urea, etc. The solid material is calculated as 100% by mass fraction, including 95%, bran 4%, urea 0.3%, KH 2 PO 4 0.7%, mix all components together and stir evenly, then add water, the mass ratio of material to water is 1:2, then stir evenly, the initial pH value is 6.5, sterilize at 121°C for 40min, and set aside.

[0038] 3. Solid fermentation

[0039] Inoculate A. utilis in the solid culture medium of jitake mushroom chaff, and inoculate 20ml of A. utilis in every kilogram of jitake mushroom chaff solid medium, about 1.2×10 8 ~1×10 9 A. utilis; i...

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PUM

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Abstract

The invention discloses a method for producing a protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and the protein feed being produced via the method for producing the protein feed through hypsizygus marmoreus fungus chaff solid state fermentation. According to the method, hypsizygus marmoreus is selected to cultivate the produced waste material-pollution-free hypsizygus marmoreus fungus chaff, the ground hypsizygus marmoreus is combined with bran, urea and the like, candida utilis, candida tropicalis and geotrichum candidum are inoculated according to a certain rate so as to ferment under an appropriate condition, and the feed which is rich in protein is obtained. After assay determination, the protein feed contains 20%-40% of crude protein which is increased by 1.5-4 times compared with the crude protein of unfermented hypsizygus marmoreus fungus chaff; the crude fiber content is between 15% and 18% which is reduced by 2-3.5 times of the crude fiber content of the unfermented hypsizygus marmoreus fungus chaff, so that the protein feed is a hypsizygus marmoreus fungus chaff protein feed which has rich nutrition, high protein and low fiber and is beneficial to the utilization and absorption of livestocks. By the method, the feeding cost of farmers can be lowered, waste utilization is realized, and an environmental protection function is obtained.

Description

technical field [0001] The invention belongs to the field of edible fungus waste reuse, and in particular relates to a method for producing protein feed by solid-state fermentation of shimeji mushroom chaff and the produced protein feed. Background technique [0002] With the development of animal husbandry, the demand for feed is increasing, and the production and utilization of protein feed has become a global hot issue. In the development and utilization of protein feed, the research of single-cell protein is attached great importance at home and abroad, and various wastes are actively used, such as: potato dregs, traditional Chinese medicine dregs, distiller’s grains, crop straw and catering waste to produce protein feed (Shi Qiyun, 2004 ; Wang Jianjun, 2007). [0003] Hypsizygus marmoreus is a rare edible fungus species favored by domestic and foreign markets in recent years, and it is also the main edible fungus species produced in factories in my country. Its market ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 李挺宋斌李泰辉沈亚恒林敏邓旺秋
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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