Compound microorganism bacterium agent and preparation method and application thereof

A technology of compound microbial bacterial agent and bacterial agent, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of improving utilization rate, continuous effect, and reducing pollution

Inactive Publication Date: 2016-02-17
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The food waste filtrate is made into compound microbial fertilizer through microbial fermentation, especially emphasizing the role of microorganisms. However, because the food filtrate has a high content of COD, s

Method used

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  • Compound microorganism bacterium agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 is used to illustrate the composite microbial bacterial agent of the present invention.

[0044] The compound microbial bacterial agent comprises:

[0045] Enterococcus faecalis VTJ2 (Enterococcus Faecalis), the number of live bacteria is 1×10 8 -5×10 8 cfu / mL;

[0046] Bacillus subtilis VTL-2 (Bacillus subtilis), the number of viable bacteria is 1.5×10 8 -2.5×10 8 cfu / mL;

[0047] Bacillus megaterium VTL-1 (Bacillus megatherium), the number of viable bacteria is 1×10 8 -2×10 8 cfu / mL.

Embodiment 2

[0049] Example 2 is used to illustrate the preparation method of the composite microbial bacterial agent of the present invention.

[0050] Including the following steps:

[0051] 1) Solid slope culture: Inoculate Bacillus subtilis and Bacillus megaterium in beef extract peptone solid medium, Enterococcus lactis in MRS solid medium, and culture at 28°C for 1 day to activate the strains;

[0052] Among them: beef extract peptone solid medium formula is: beef extract 5g, peptone 10g, NaCl 5g, distilled water 1000mL, solid plus agar 20g, pH7.0-7.2;

[0053] The formula of MRS medium is: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 801.0mL, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate heptahydrate 0.58g, tetrahydrate Manganese sulfate 0.25g, distilled water 1000mL, pH6.2-6.6;

[0054] 2) Primary seed culture: inoculate the activated Bacillus subtilis and Bacillus megaterium strains in the step 1) respective...

Embodiment 3

[0062] Example 3 is used to illustrate another optional preparation method of the composite microbial agent of the present invention.

[0063] Including the following steps:

[0064] 1) Solid slope culture: Bacillus subtilis and Bacillus megaterium were inoculated in beef extract peptone solid medium, Enterococcus lactic acid faecalis was inoculated in MRS solid medium, and cultured at 30°C for 3 days to activate the strains;

[0065] Among them: beef extract peptone solid medium formula is: beef extract 5g, peptone 10g, NaCl 5g, distilled water 1000mL, solid plus agar 20g, pH7.0-7.2;

[0066] The formula of MRS medium is: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 801.0mL, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate heptahydrate 0.58g, tetrahydrate Manganese sulfate 0.25g, distilled water 1000mL, pH6.2-6.6;

[0067] 2) Primary seed culture: Inoculate the activated Bacillus subtilis and Bacillus mega...

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Abstract

The invention relates to the field of microbial preparations and particularly discloses a compound microorganism bacterium agent and a preparation method and application thereof. The compound microorganism bacterium agent comprises lactic acid enterococcus faecalis, bacillus subtilis and bacillus megaterium, wherein the total viable count of the agent is not less than 3.5*108 cfu/mL. The compound microorganism bacterium agent is composed of reasonable strains according to composition characteristics of kitchen filtrate, can quickly degrade harmful substances in the kitchen filtrate, has a remarkable effect on degrading COD, BOD, ammonia nitrogen and the like in the kitchen filtrate and can effectively degrade organic matter to greatly relieve odor of the kitchen filtrate; the processed kitchen filtrate can also be used as a raw material of compound microorganism water-soluble fertilizer, so that the utilization rate of energy sources is increased, and environmental pollution caused by direct discharge of the kitchen filtrate is reduced.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a composite microbial preparation and its preparation method and application. Background technique [0002] With the development of the city, various wastes and garbage also emerge as the times require, among which kitchen waste is an important part. The current general method of dealing with food waste is to separate the food waste into solid and liquid, and process the solid part into feed, fertilizer, etc., while the liquid part is mostly discharged directly or enters the sewage treatment system. Studies have found that the direct discharge of a large amount of organic matter in the kitchen filtrate will pollute the groundwater, and the generated methane and strong odor will seriously affect people's life and health. Research has found that kitchen filtrate is rich in organic matter and nitrogen sources, and the reuse of these substances is not only of great significance...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F7/00C12R1/46C12R1/125C12R1/11
CPCY02A40/20
Inventor 杨军任莉王凯彭生平
Owner 北京沃土天地生物科技股份有限公司
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