Composite lactobacillus agent for improving grape quality and preparation method of composite lactobacillus agent

A technology of compound lactic acid bacteria and bacteria agent, applied in the field of compound lactic acid bacteria bacteria agent and its preparation, can solve the problems of increased fertilization cost, decline of soil fertility and quality, and not necessarily high sugar content, so as to increase planting benefits, promote growth metabolism, promote production and The effect of quality traits

Active Publication Date: 2016-12-14
金华银河生物科技有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods to increase grape yield are mainly planting techniques such as selecting suitable varieties, strengthening water and fertilizer management, and preventing and controlling diseases and insect pests. According to the growth characteristics of grapes and the needs of commodity traits, the application of different fertilizers is a necessary condition for high-yield and high-quality grapes. Research on different fer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite lactobacillus agent for improving grape quality and preparation method of composite lactobacillus agent
  • Composite lactobacillus agent for improving grape quality and preparation method of composite lactobacillus agent
  • Composite lactobacillus agent for improving grape quality and preparation method of composite lactobacillus agent

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] Yet another embodiment of the present invention provides the preparation method of above-mentioned composite lactic acid bacteria inoculum, and concrete method step is as follows:

[0031] S1: First inoculate the inoculum of 4-10% (v / w) of the Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in the MRS medium at 37±1°C Cultivate under conditions for 26-28 hours, centrifuge and wash 3 times with sterile physiological saline, add 8%-12% sterilized skim emulsion to the obtained bacterial precipitate, and adjust the number of bacteria to 1.8-2.0×10 9 cfu / ml, mix well and culture at 35-37°C for 35-48 hours.

[0032] In this step, the above two bacteria can be fully cultivated to avoid antagonism.

[0033] S2: Mix the strains obtained in the above steps with Lactobacillus delbrueckii subsp.bulgaricus according to the ratio of (3-4):(1-1.5), and mix them with 8-12% (v / w) The amount of inoculum was inoculated into MRS medium, cultured at 39±1°C for 12-24 hours, and 5...

Embodiment 1

[0038] Embodiment 1: The compound lactic acid bacteria agent is composed of plant Lactobacillus (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus delbrueckii subsp.bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus) according to 1.5:1.5:1 Ratio composition, the total number of live bacteria content of the compound lactic acid bacteria agent is 2.4×10 9 cfu / ml.

Embodiment 2

[0039] Embodiment 2: the composite lactic acid bacteria agent is composed of Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus delbrueckii subsp. Ratio composition, the total number of live bacteria content of the compound lactic acid bacteria agent is 2.8×10 9 cfu / ml.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a composite lactobacillus agent for improving grape quality and a preparation method of the composite lactobacillus agent, and belongs to the field of application of microorganisms. The composite lactobacillus agent consists of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus delbrueckii subsp.bulgaricus in a ratio of (1.5-2):(1.5-2):(1-1.5); the total viable count of the composite lactobacillus agent is 2.4-3.2*10<9>cfu/ml. Compounding of the three types of bacteria has a significant effect of promoting growth and metabolism of grapes, and particularly can synergistically increase the total soluble sugar content and the soluble solid content of the grapes, so that the grape quality is improved and planting benefits are enhanced.

Description

technical field [0001] The invention relates to the field of microbial application, in particular to a compound lactic acid bacteria agent for improving grape quality and a preparation method thereof. Background technique [0002] At present, nearly half of the countries in the world, including major developed countries such as Japan, the United Kingdom, the United States, France, and Sweden, and developing countries such as Pakistan, India, and China, are developing and utilizing microbial fertilizers. Yao Guoqiang screened out strains that inhibit pathogenic fungi through preliminary experiments, and obtained lactic acid bacteria and their metabolites through low-temperature and long-term mixed fermentation. Yu Xiangrong and others screened the fermentation liquid of three strains of lactic acid bacteria with good antibacterial effect and wide antibacterial spectrum, which have good control effects on Guifei rose downy mildew, Meile anthracnose and Fujiminori white rot. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/25C12R1/245C12R1/225
CPCA01N63/00C12N1/20
Inventor 李海梅赵金山刘占民邹晓霞李士美任贻超张晓军谢宝琦
Owner 金华银河生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap