Composite lactobacillus agent for improving grape quality and preparation method of composite lactobacillus agent
A technology of compound lactic acid bacteria and bacteria agent, applied in the field of compound lactic acid bacteria bacteria agent and its preparation, can solve the problems of increased fertilization cost, decline of soil fertility and quality, and not necessarily high sugar content, so as to increase planting benefits, promote growth metabolism, promote production and The effect of quality traits
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[0030] Yet another embodiment of the present invention provides the preparation method of above-mentioned composite lactic acid bacteria inoculum, and concrete method step is as follows:
[0031] S1: First inoculate the inoculum of 4-10% (v / w) of the Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in the MRS medium at 37±1°C Cultivate under conditions for 26-28 hours, centrifuge and wash 3 times with sterile physiological saline, add 8%-12% sterilized skim emulsion to the obtained bacterial precipitate, and adjust the number of bacteria to 1.8-2.0×10 9 cfu / ml, mix well and culture at 35-37°C for 35-48 hours.
[0032] In this step, the above two bacteria can be fully cultivated to avoid antagonism.
[0033] S2: Mix the strains obtained in the above steps with Lactobacillus delbrueckii subsp.bulgaricus according to the ratio of (3-4):(1-1.5), and mix them with 8-12% (v / w) The amount of inoculum was inoculated into MRS medium, cultured at 39±1°C for 12-24 hours, and 5...
Embodiment 1
[0038] Embodiment 1: The compound lactic acid bacteria agent is composed of plant Lactobacillus (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus delbrueckii subsp.bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus) according to 1.5:1.5:1 Ratio composition, the total number of live bacteria content of the compound lactic acid bacteria agent is 2.4×10 9 cfu / ml.
Embodiment 2
[0039] Embodiment 2: the composite lactic acid bacteria agent is composed of Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus delbrueckii subsp. Ratio composition, the total number of live bacteria content of the compound lactic acid bacteria agent is 2.8×10 9 cfu / ml.
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