Compound microbial agent as well as preparation method and use method thereof

A technology of composite microbial agent and viable count, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increased operating costs and inconvenient operation, and achieve fast reproduction speed and strong adaptability , Increase the concentration of active strains and the effect of metabolic activity

Inactive Publication Date: 2016-08-24
SHANDONG WENYUAN BUILDING MATERIALS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composite microbial agent needs to add 10%-20% of the initial dosage in each sewage treatment cycle
The addition of bacterial agents in each cycle means that the o

Method used

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  • Compound microbial agent as well as preparation method and use method thereof
  • Compound microbial agent as well as preparation method and use method thereof
  • Compound microbial agent as well as preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: According to parts by weight, weigh or measure 0.11 parts of dipotassium hydrogen phosphate, 0.04 parts of potassium dihydrogen phosphate, 0.025 parts of ammonium chloride, 0.07 parts of magnesium sulfate, 0.03 parts of calcium chloride, 0.00025 parts of ferric chloride, 0.00003 parts of manganese, 0.00004 parts of zinc sulfate, 0.00003 parts of ammonium molybdate, and 1000 parts of water are prepared into culture medium;

[0041] Step 2: Inoculate Halomonas denitrifying bacteria, Halovibrio denitrifying bacteria, Achromobacter denitrifying bacteria, Bacillus thuringiensis, Bacillus subtilis, etc. into the culture medium, the inoculum amount is 10%, and add the carrier to control the fermentation The temperature is 35°C, and the fermentation culture is 48h;

[0042] Step 3: After the fermentation is over, count the live bacteria on the plate and measure the weight. According to the weight ratio, carrier: total live bacteria=5:1 is added to the carrier for adsor...

Embodiment 2

[0046] Step 1: According to parts by weight, weigh or measure 0.11 parts of dipotassium hydrogen phosphate, 0.04 parts of potassium dihydrogen phosphate, 0.025 parts of ammonium chloride, 0.07 parts of magnesium sulfate, 0.03 parts of calcium chloride, 0.00025 parts of ferric chloride, 0.00003 parts of manganese, 0.00004 parts of zinc sulfate, 0.00003 parts of ammonium molybdate, and 1000 parts of water are prepared into culture medium;

[0047] Step 2: Inoculate Halomonas denitrifying bacteria, Halovibrio denitrifying bacteria, Achromobacter denitrifying bacteria, Bacillus thuringiensis, Bacillus subtilis, etc. into the culture medium, the inoculum amount is 10%, and add the carrier to control the fermentation The temperature is 34°C, and the fermentation culture is 48h;

[0048] Step 3: After the fermentation is over, count the live bacteria on the plate and measure the weight. According to the weight ratio, carrier: total viable bacteria = 6: 1 is added to the carrier for ...

Embodiment 3

[0052] Step 1: According to parts by weight, weigh or measure 0.11 parts of dipotassium hydrogen phosphate, 0.04 parts of potassium dihydrogen phosphate, 0.025 parts of ammonium chloride, 0.07 parts of magnesium sulfate, 0.03 parts of calcium chloride, 0.00025 parts of ferric chloride, 0.00003 parts of manganese, 0.00004 parts of zinc sulfate, 0.00003 parts of ammonium molybdate, and 1000 parts of water are prepared into culture medium;

[0053] Step 2: Inoculate Halomonas denitrifying bacteria, Halovibrio denitrifying bacteria, Achromobacter denitrifying bacteria, Bacillus thuringiensis, Bacillus subtilis, etc. into the culture medium, the inoculum amount is 10%, and add the carrier to control the fermentation The temperature is 33°C, and the fermentation culture is 48h;

[0054] Step 3: After the fermentation is over, count the live bacteria on the plate and measure the weight. According to the weight ratio, carrier: total viable bacteria = 7: 1 is added to the carrier for ...

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Abstract

The invention discloses a compound microbial agent which is prepared from nitrifying bacteria, nitrosomonas, single cell bacteria denitrification salt, salt denitrifying bacterium vibrio cholera, achromobacter denitrificans, bacillus thuringiensis and bacillus subtillis, wherein the total viable count is more than 5.9x10<10>cfu/g; the nitrifying bacteria and the nitrosomonas account for 10 to 20 percent of the total viable count; the single cell bacteria denitrification salt, the salt denitrifying bacterium vibrio cholera and achromobacter denitrificans account for 15 to 20 percent of the total viable count; the bacillus thuringiensis and the bacillus subtillis account for 5 to 10 percent of the total viable count. The compound microbial agent has the benefits that the compound microbial agent is fed at a time, and no bacteria is required to be kept added after the system is stabilized; therefore, the operation cost of sewage treatment is reduced, and the convenience of sewage treatment operation is enhanced.

Description

technical field [0001] The invention relates to the field of sewage biological treatment, in particular to a composite microbial agent and a preparation method and application method thereof. Background technique [0002] The Chinese patent with the notification number 102745821B discloses a composite microbial agent for sludge reduction and its preparation method and application. The composite microbial agent can reduce the amount of sludge from the source without affecting the quality of effluent, so that the discharge of excess sludge in the sewage treatment process is greatly reduced. However, the composite microbial bacterial agent needs to add 10%-20% of the initial dosage in each sewage treatment cycle. On the one hand, the addition of bacterial agents in each cycle means that the operating cost will increase with the extension of the operating time, and on the other hand, it will cause inconvenience in operation, which needs to be improved. Contents of the inventi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/125C12R1/63C12R1/025
CPCC12N1/20C02F3/341
Inventor 齐文王勇虞志伟边玉彬陶贵金岳登旭
Owner SHANDONG WENYUAN BUILDING MATERIALS TECH
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