Microbial preparation for gangue reclaimed soil and method for reclaiming gangue soil by using microbial preparation

A microbial preparation and waste rock technology, which is applied to the special microbial preparation for waste rock reclamation soil and the application field on coal mine waste rock, can solve the problems of unfavorable accumulation of organic matter in waste rock, small number of soil microorganisms, and difficult growth of crops, etc., and achieve a solution Spontaneous combustion problems, low cost, effects that reduce effectiveness

Active Publication Date: 2015-05-20
INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is generally used in production to "discharge coal gangue in layers, cover soil and roll it", and then carry out vegetation construction, but practice has proved that this planting mode of covering soil still has many defects: 1) gangue hills are poor in nutrients, and crops are difficult to grow; 2) The gangue hill has a high ground temperature, a small number of soil microorganisms, and a simple microbial community, which is not conducive to the accumulation of organic matter in the gangue hill; 3) After the gangue hill is covered with soil and rolled, there will still be oxygen, which will cause the gangue hill to spontaneously combust

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] There are six kinds of microorganisms involved in the preparation of microbial preparations, namely Bacillus subtilis ACCC10242, Bacillus licheniformis ACCC01193, Bacillus megaterium ACCC11107, and Bacillus mucilaginosus. ) ACCC10013, Azotobacter chroococcum ACCC10006, Lysinibacillus fusiformis CGMCC1.3602, all freeze-dried strains, are commercially available products.

[0026] The target strains were activated, slant culture, liquid shake flask and fermenter culture in sequence under aseptic conditions.

[0027] 1) Activation. Under aseptic conditions, wipe the mash tube containing freeze-dried preserved microbial strains with absorbent cotton soaked with 75% alcohol, heat the top of the mash tube with a flame, and drip a small amount of sterile water to the heated top to break it. Use tweezers to knock open the broken condiment tube. Use a sterile pipette to suck up 0.3-0.5 mL of appropriate liquid culture medium, drop it into the ampoule tube, and gently shake to dissol...

Embodiment 2

[0032] Weigh 50g of Bacillus subtilis and 50g of Bacillus licheniformis, mix with 150g of diatomaceous earth and 150g of bentonite, stir evenly with a stirrer, and place in a sterile ventilated room at 15~50℃ to ventilate and dry until moisture content 2×10 8 CFU / g.

[0033] Weigh 50g of Bacillus jelly-like sludge and 75g of Bacillus licheniformis sludge, mix them with 175g of diatomaceous earth and 200g of bentonite, add 75g of calcium magnesium phosphate fertilizer, stir evenly with a stirrer at a constant speed, and place at 15-50℃ in aseptic ventilation The room is ventilated and dried until the water content is less than 10%, and the type Ⅱ microbial preparation is obtained, and the effective number of viable bacteria is more than 2×10 8 CFU / g.

[0034] Weigh 25g of Azotobacter Mud, 25g of Bacillus megaterium, 50g of Bacillus jelly, 25g of Bacillus lysine, and mix with 150g of straw powder, 100g of wood chips, and 125g of turf soil 85g calcium magnesium phosphate fertilizer,...

Embodiment 3

[0036] Weigh 50g of Bacillus subtilis and 75g of Bacillus licheniformis, mix them with 200g of diatomaceous earth and 175g of bentonite, stir evenly with a stirrer, and place them in a sterile ventilation room at 15~50℃ for ventilation and drying to moisture content 2×10 8 CFU / g.

[0037] Weigh 75g of Bacillus jelly-like sludge and 75g of Bacillus licheniformis sludge, mix with 200g of diatomaceous earth and 250g of bentonite, add 90g of calcium-magnesium phosphate fertilizer, stir evenly with a stirrer at a constant speed, and place at 15-50℃ in aseptic ventilation The room is ventilated and dried until the water content is less than 10%, and the type II microbial preparation is obtained, and the effective number of viable bacteria is more than 2×10 8 CFU / g.

[0038] Weigh 50g of Azotobacter Mud, 75g of Bacillus megaterium, 125g of Bacillus jelly, 100g of Bacillus lysine, and mix with 350g of straw powder, 350g of wood chips, and 350g of turf soil. Calcium magnesium phosphate f...

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Abstract

The invention discloses a microbial preparation for gangue reclaimed soil. The microbial preparation comprises three microbial preparations which are respectively used independently, wherein the I type microbial preparation contains bacillus subtilis and bacillus licheniformis; the II type microbial preparation contains bacillus mucilaginosus and the bacillus licheniformis; the III type microbial preparation contains azotobacter chroococcum, bacillus megaterium, the bacillus mucilaginosus and lysine bacillus; the total viable count of every gram of the microbial preparation is (2-200)*10<8> CFU. The gangue reclaimed soil is covered in layers by using the microbial preparation disclosed by the invention, a first gangue layer, a sticky loess and fly ash mixing covering layer, a second gangue layer, a fly ash covering layer, a fine granular gangue layer and a loess covering layer are sequentially arranged from bottom to top, the I type microbial preparation is applied to the sticky loess and fly ash mixing covering layer, the II type microbial preparation is applied to the fly ash covering layer, the III type microbial preparation is applied to the loess covering layer, and crops are planted on the loess covering layer.

Description

Technical field [0001] The invention belongs to the technical field of gangue hill reclamation, and in particular relates to a special microorganism preparation for gangue hill reclamation soil and its application on coal gangue hills. Background technique [0002] Coal gangue is waste generated during coal mining, accounting for about 20% of coal mining volume. At present, the comprehensive utilization rate of coal gangue in my country is about 30-40%, most of which are piled up nearby as waste, forming gangue hills of different sizes, occupying the land and destroying the landscape. The gangue hill has become the area with the worst site conditions, the largest stacking volume, and the most difficult governance in the coal mine area. There are more than 5,000 gangue hills in the country, and about 1 / 3 of the gangue hills have spontaneous combustion of different degrees. The spontaneous combustion of coal gangue directly affects its reclamation effect and poses a certain threa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/125C12R1/10C12R1/07C12R1/11
CPCC05B17/00C05G3/00C12N1/20
Inventor 郜春花李建华张强靳东升卢朝东卢晋晶董云中
Owner INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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