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Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof

A compound microbial agent and high ammonia nitrogen technology, applied in the field of microorganisms, can solve the problems of ineffective improvement of water body material circulation, high operating cost of activated sludge method, unsatisfactory ammonia nitrogen removal rate, etc., to achieve improved chroma removal rate, Improve the quality of effluent and reduce the effect of odor

Inactive Publication Date: 2016-10-26
湖北凌卓生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The activated sludge method that has emerged in the past few years has been widely used in sewage treatment, but the operating cost of the activated sludge method is high, and the removal rate of COD (Chemical Oxygen Demand, Chemical Oxygen Demand) and ammonia nitrogen is not ideal.
[0004] In recent years, microbial preparations have been widely used in the environmental field, but there are still many problems, such as relatively single microbial strains, which can only degrade specific pollutants, cannot effectively improve the material circulation of water bodies, and the treatment effect is not ideal

Method used

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  • Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof

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Effect test

Embodiment 1

[0028] A compound microbial bacterial agent for treating sewage containing high ammonia nitrogen is prepared by the following method:

[0029](1) Solid slant culture: Inoculate nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis in nitrifying solid medium, nitrosifying solid medium and beef extract peptone medium respectively, and culture them at a constant temperature of 28°C for 4 days to make the strains fully Activation; the nitrated solid medium is: potassium nitrate 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~7.2 The nitrosation solid medium is: sodium nitrite 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~ 7.2: The beef extract peptone solid medium is: 0.5wt% beef extract, 1wt% peptone, 0.5wt% sodium chloride, 2wt% agar, the balance is water, and the...

Embodiment 2

[0038] A compound microbial bacterial agent for treating sewage containing high ammonia nitrogen is prepared by the following method:

[0039] (1) Solid slant culture: Inoculate nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis in nitrifying solid medium, nitrosifying solid medium and beef extract peptone medium respectively, and culture them at a constant temperature of 29°C for 5 days to make the strains fully Activation; the nitrated solid medium is: potassium nitrate 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~7.2 The nitrosation solid medium is: sodium nitrite 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~ 7.2: The beef extract peptone solid medium is: 0.5wt% beef extract, 1wt% peptone, 0.5wt% sodium chloride, 2wt% agar, the balance is water, and th...

Embodiment 3

[0048] A compound microbial bacterial agent for treating sewage containing high ammonia nitrogen is prepared by the following method:

[0049] (1) Solid slant culture: Inoculate nitrifying bacteria, nitrosifying bacteria and Bacillus subtilis in nitrifying solid medium, nitrosifying solid medium and beef extract peptone medium respectively, and culture them at a constant temperature of 30°C for 6 days to make the strains fully Activation; the nitrated solid medium is: potassium nitrate 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~7.2 The nitrosation solid medium is: sodium nitrite 0.8wt%, ammonium sulfate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, agar 2wt%, the balance is water, and the pH is 7.0~ 7.2: The beef extract peptone solid medium is: 0.5wt% beef extract, 1wt% peptone, 0.5wt% sodium chloride, 2wt% agar, the balance is water, and th...

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Abstract

The invention belongs to the field of microbial technology and specifically relates to a composite microbial agent for treating high-ammonia-nitrogen sewage and a preparation method and application thereof. The composite microbial agent contains nitrobacteria, nitrite bacteria and bacillus subtilis. The viable count of nitrobacteria is 1.0*108-2*108 cfu / mL; the viable count of nitrite bacteria is 1.0*108-1.5*108 cfu / mL; and the viable count of bacillus subtilis is 1.0*108-2.0*108 cfu / mL. The total viable count is 3.0*108-5.5*108 cfu / mL. The composite microbial agent is used for treating high-ammonia-nitrogen sewage, is simple to use, is low-cost and has significant effects. The highest removal rate of ammonia nitrogen in sewage reaches 98%, COD removal rate reaches 85%, BOD removal rate reaches 92%, removal rate of total sulfur reaches 90%, and removal rate of sewage chroma reaches 32%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite microbial bacterial agent for treating sewage containing high ammonia nitrogen, a preparation method and application thereof. Background technique [0002] The eutrophication of water body has become a problem that people pay more and more attention to, and the state has strict regulations and requirements on sewage discharge standards. As an important indicator of water body, ammonia nitrogen has been widely concerned about its treatment. Similar to COD, ammonia nitrogen is also the main oxygen-consuming pollutant in water bodies. The oxidative decomposition of ammonia nitrogen consumes dissolved oxygen in the water body, thus making the water body black and smelly. The non-ionic ammonia in ammonia nitrogen is highly toxic to aquatic organisms, and some studies have shown that its harm is dozens of times greater than that of ammonium salts. At th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C02F101/16
CPCC12N1/20C02F3/341C02F2101/16
Inventor 徐凯杨军李靖
Owner 湖北凌卓生物工程有限公司
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