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Method for producing lactobacillus sp. probiotics

A technology of lactobacillus and production method, applied in the field of aquaculture, can solve the problems of reducing production cost, complex medium composition and high production cost

Inactive Publication Date: 2011-01-19
TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the culture of Lactobacillus, the composition of the medium is complex, resulting in high production costs, and the formulation of the medium needs to be optimized to reduce production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] The total number of live bacteria per milliliter of Lactobacillus cultured at 32°C for 68 hours on a liquid medium of 7g of peptone, 4g of yeast extract, 14g of sucrose, 1.1g of potassium dihydrogen phosphate, 230ml of tomato juice, and 770ml of water is 9.9×10 10 cfu / ml. .

example 2

[0018] The total number of live bacteria per milliliter of Lactobacillus cultured at 29°C for 65 hours on a liquid medium of 6 g of peptone, 7 g of yeast extract, 18 g of sucrose, 2.9 g of potassium dihydrogen phosphate, 50 ml of tomato juice, and 950 ml of water is 7.7×10 10 cfu / ml.

example 3

[0020] The total number of live bacteria per milliliter of Lactobacillus cultured at 30°C for 72 hours on a liquid medium of 9 g of peptone, 3 g of yeast extract, 5 g of sucrose, 1.4 g of potassium dihydrogen phosphate, 290 ml of tomato juice, and 710 ml of water is 5.9×10 10 cfu / ml.

[0021] The formula of the lactobacillus culture medium is easy to operate and low in cost, so that the total number of viable bacteria per milliliter of the product is 9.9-1.0×10 10 cfu / ml.

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PUM

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Abstract

The invention relates to a formula of a culture medium of lactobacillus sp.. The lactobacillus sp. is cultured at the temperature of between 28 and 37 DEG C for 60 to 72 hours in a liquid culture medium comprising the following basic components: 4 to 9g of peptone, 3 to 8g of yeast extract, 5 to 20g of sucrose, 0.5 to 3.0g of potassium dihydrogen phosphate, 50 to 300ml of tomato juice and 700 to 950ml of water, so that the total viable count of each millimeter of product is 9.9-1.0*10<10>cfu / ml. When the culture medium is used for producing the lactobacillus sp., the preparation method is simple and easy to operate and has low cost; and the total viable lactobacillus sp. count of each millimeter of product is 9.9-1.0*10<10>cfu / ml.

Description

technical field [0001] The invention belongs to the field of breeding technology and relates to a feed additive, a probiotic feed additive and a production method thereof, in particular to a production method of Lactobacillus sp. probiotics. Background technique [0002] Probiotics are also known as prebiotics, probiotics, libiotics, biotics, econazole, live bacteria preparations, etc. Prebiotics are selected from the dominant species in the normal intestinal flora of animals. After a series of artificial cultures, they are made into live bacterial preparations, and then returned to the intestinal environment of animals to exert their inherent physiological effects. Prebiotics inhibit the proliferation of harmful bacteria by competing with harmful bacteria for oxygen, nutrients or colonization sites, thereby improving the balance of intestinal flora and purifying the intestinal environment. Through anti-oxidation, anti-mutation, physiological activity and immune stimulation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225
Inventor 范寰
Owner TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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