Artificial feed for tomato leafminer and preparation method and application thereof

Abstract

The invention discloses an artificial feed for tomato leafminers, and a preparation method and application thereof. The feed for tomato leafminers comprises cellulose, lactic acid casein, wheat germ powder, Wesson salt, soybean powder, agar, sucrose, glucose, cholesterol, linolenic acid, linoleic acid, soybean oil, a composite preservative solution, a composite antibiotic solution, a composite vitamin B solution, a host plant homogenate and distilled water. The main raw materials of the feed are low in price, the preparation method of the feed is simple, the obtained artificial feed is mildew-proof and antiseptic, and the feeding effect is basically consistent with the level of the natural host. The larvae cultured with the feed are healthy and uniform in age, and the purpose of supplying a standard insect source for tomato leafminers can be achieved throughout the year and in successive generations.

Description

Technical Field

[0001] The invention relates to the field of artificially reared pests, and in particular to an artificial feed for tomato leafminers and a preparation method and application thereof. Background Art

[0002] Tomato leafminer Phthorimaea ( Tuta ) absoluta Meyrick belongs to the family Gelechiidae of the order Lepidoptera. It is an important worldwide quarantine pest, especially posing a serious threat to the production of fresh tomatoes in open fields and protected areas. The insect not only directly feeds on greenhouse and open-field tomatoes, but also causes secondary damage by pathogens, resulting in a serious decline in tomato yield and quality. The damaged tomato fruits will lose their economic value, with economic losses of up to 50%-100%. The tomato leafminer has become one of the serious pests restricting tomato production in my country. The insect has a wide range of host plants, including 9 families (Solanaceae, Leguminosae, Malvaceae, Amaranthaceae, Chenopodiaceae, Asteraceae, Convolvulaceae, Cruciferae, and Poaceae) and about 40 species / genera. On October 23, 2023, the Crop Production Management Department of the Ministry of Agriculture and Rural Affairs, in accordance with the relevant provisions of the Regulations on the Prevention and Control of Crop Diseases and Insects, plans to include the tomato leafminer in the "List of Class I Crop Diseases and Insects" for management.

[0003] Research on biological control of tomato leafminers has increased the demand for artificially raised tomato leafminers year by year. The most common method of raising tomato leafminers is to feed them with tomato seedlings. The cost of feeding with tomato seedlings is high and the cycle is long. It is difficult to distinguish the larval stages and obtain tomato leafminers in the pupal stage. It is difficult to control the temperature and humidity, which can easily cause the larvae to get sick and reduce the survival rate. It is limited by the variety of tomato seedlings. First, tomato leafminers are boring pests. When feeding, the larvae will bore into tomato leaves. When conducting experiments, the larvae must be peeled out of the tubers. The 1-2 instar larvae are small in size and difficult to peel off, which can easily cause the larvae to be disabled or killed, and it takes time. Secondly, the eggs of tomato leafminers are generally laid on the front of the leaves and the inside of the stems of the host plants. The hairs on the leaves of tomato seedlings are long and dense, making it difficult to separate the eggs. The fourth instar larvae of tomato leafminers usually fall into the soil and wrap the plant soil debris to pupate. It is not easy to distinguish the pupae of tomato leafminers when raising tomato seedlings. Tomato seedlings are mainly cultivated in the laboratory and are easily attacked by the external environment and other insects. Different batches of tomato seedlings have different nutritional components, which will lead to differences in the physiological indicators of tomato leafminers, especially digestive physiological indicators, which is not conducive to toxicological, physiological and ecological experiments. As an important quarantine pest, the breeding of tomato leafminers must be strictly in accordance with the relevant regulations of "Biosafety". The current breeding conditions are usually carried out in insect cages. The discarding of breeding soil and the non-standard specifications of insect cages are extremely likely to cause the insect to escape, helping the pest to spread further. In summary, there is an urgent need for a convenient, safe and easy-to-control propagation method for breeding the insect. Summary of the invention

[0004] The present invention aims to make up for the shortcomings of the prior art and provide an artificial feed for tomato leafminers and a preparation method thereof. The main raw materials of the artificial feed are cheap and easily available, the preparation method of the feed is simple, the larvae cultured with the feed are healthy and of uniform age, and the purpose of year-round, successive generation breeding and feeding, and year-round supply of standard insect sources for tomato leafminers can be achieved.

[0005] In order to achieve the above object, the technical solution provided by the present invention is as follows:

[0006] The first aspect of the present invention provides a tomato leafminer feed, which comprises cellulose, lactic acid casein, wheat germ powder, Wesson salt, soybean meal, agar, sucrose, glucose, cholesterol, linolenic acid, linoleic acid, soybean oil, a composite preservative solution, a composite antibiotic solution, a composite vitamin B solution, a host plant homogenate and distilled water.

[0007] Preferably, the vitamin B complex solution includes riboflavin, lutein, KOH solution, niacinamide, calcium pantothenate, thiamine hydrochloride, pyridoxine hydrochloride, biotin, vitamin B 12 and distilled water.

[0008] Preferably, the compound antibiotic solution comprises tetracycline, penicillin, metronidazole and distilled water.

[0009] Preferably, the composite preservative comprises methyl paraben, propionic acid, sodium acetate and 75% ethanol solution.

[0010] Preferably, the host plant homogenate comprises host plant, vitamin C and purified water obtained by squeezing juice.

[0011] Further preferably, the host plant includes but is not limited to tomato.

[0012] Preferably, the agar includes but is not limited to agar powder, agar flakes and other neutral additive plasticizers with gelling power.

[0013] Preferably, the feed comprises, by weight: 40-55 parts of cellulose, 12-18 parts of lactic acid casein, 12-18 parts of wheat germ powder, 3-4 parts of Wesson salt, 4-6 parts of soybean meal, 10-15 parts of agar, 10-15 parts of sucrose, 1-2 parts of glucose, 0.4-0.5 parts of cholesterol, 0.8-1.2 parts of linolenic acid, 0.8-1.2 parts of linoleic acid, 1-2 parts of soybean oil, 8-10 parts of complex vitamin B solution, 150-200 parts of host plant homogenate, 0.5-2 parts of complex antibiotic solution, 5-10 parts of complex preservative solution and 350-500 parts of distilled water.

[0014] In a specific embodiment, the feed comprises, by weight: 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 3 parts of Wesson salt, 5 parts of soybean powder, 14 parts of agar, 10 parts of sucrose, 2 parts of glucose, 0.5 parts of cholesterol, 10 parts of composite preservatives, 1 part of composite antibiotic solution, 1 part of linolenic acid, 1 part of linoleic acid, 2 parts of soybean oil, 10 parts of composite vitamin B solution, 200 parts of host plant homogenate and 400 parts of distilled water.

[0015] Preferably, the vitamin B complex solution comprises, by weight: 4-6 parts of riboflavin, 1.5-3.5 parts of lutein, 4-6 parts of 1 mol / L KOH solution, 0.5-2.0 parts of nicotinamide, 0.5-2.0 parts of calcium pantothenate, 1.5-3.5 parts of thiamine hydrochloride, 1.5-3.5 parts of pyridoxine hydrochloride, 0.1-0.5 parts of biotin, vitamin B 12 0.1-0.5 parts, distilled water 500-1000 parts.

[0016] In one embodiment, the vitamin B complex solution comprises, by weight: 5 parts of riboflavin, 2.5 parts of lutein, 5 parts of 1 mol / L KOH solution, 1 part of niacinamide, 1 part of calcium pantothenate, 2.5 parts of thiamine hydrochloride, 2.5 parts of pyridoxine hydrochloride, 0.2 parts of biotin, and vitamin B 12 0.2 parts, 950 parts of distilled water.

[0017] Preferably, the compound antibiotic solution comprises, by weight: 4-6 parts of tetracycline, 2-5 parts of penicillin, 1-3 parts of metronidazole, and 50-150 parts of distilled water.

[0018] In a specific embodiment, the compound antibiotic solution comprises, by weight: 5 parts of tetracycline, 3 parts of penicillin, 2 parts of metronidazole, and 100 parts of distilled water.

[0019] Preferably, the composite preservative comprises, by weight: 5-15 parts of methyl parahydroxybenzoate, 4-6 parts of propionic acid, 4-6 parts of sodium acetate, and 50-150 parts of 75% ethanol solution.

[0020] In a specific embodiment, the composite preservative includes, by weight: 10 parts of methyl parahydroxybenzoate, 5 parts of propionic acid, 5 parts of sodium acetate, and 100 parts of 75% ethanol solution.

[0021] Preferably, the host plant homogenate comprises 50-150 parts of tomatoes, 50-150 parts of purified water, and 0.1-1.0 parts of vitamin C by weight.

[0022] In a specific embodiment, the host plant homogenate comprises 100 parts of tomatoes, 100 parts of purified water, and 0.5 parts of vitamin C by weight.

[0023] The second aspect of the present invention provides a method for preparing the above-mentioned tomato leafminer feed, wherein the method comprises mixing the various components in the above-mentioned tomato leafminer feed.

[0024] Preferably, the preparation method comprises:

[0025] S1. Put the host plant, purified water and vitamin C into a juicer to obtain a host plant homogenate;

[0026] S2. Homogenize cellulose, lactic acid casein, wheat germ powder, Wesson salt, soybean powder, agar, sucrose, glucose, cholesterol, composite preservative solution and host plant, add to distilled water, stir evenly, and sterilize;

[0027] S3. After the sterilized mixture in S2 is cooled, add the complex vitamin B solution, the complex antibiotic solution, linolenic acid, linoleic acid and soybean oil, stir evenly, cool until condensed, obtain the tomato leafminer feed, and evaporate excess water.

[0028] Preferably, in step S1, the raw material is placed in a juicer and operated for 1-2 min to obtain a host plant homogenate.

[0029] Preferably, the sterilization conditions in step S2 include placing in a sterilizing pot and sterilizing at 105° C. for 15-20 minutes.

[0030] Preferably, in step S3, the raw materials are added after the sterilized mixture in S2 is cooled to 60°C.

[0031] Preferably, in step S3, the obtained tomato leafminer feed is placed in a clean bench for 4-5 hours to evaporate excess water.

[0032] A third aspect of the present invention provides a method for cultivating tomato leafminer, which comprises cultivating tomato leafminer using the above-mentioned tomato leafminer feed or the tomato leafminer feed obtained by the above-mentioned preparation method.

[0033] Preferably, the culture method comprises: culturing the newly hatched larvae of tomato leafminer in feed.

[0034] The term "include" or "comprising" in the present invention is an open description containing the specified components or steps described, and other specified components or steps that will not be substantially affected.

[0035] Herein, when it comes to numerical ranges, unless otherwise specified, the numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as every value between such minimum and maximum values. Further, when a range refers to an integer, every integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0036] Beneficial effects of the present invention:

[0037] The main raw material of the invention is low in price and has a simple preparation method. It is obtained by combining cellulose, wheat germ powder, soybean powder, lactic acid casein powder and host plants to provide main nutrients required for the growth and development of tomato leafminers.

[0038] The host plant homogenate added by the present invention is more suitable for the components of the natural host and can enhance the adaptability of young larvae of tomato leafminers to the feed.

[0039] The invention adds agar to achieve good shaping and moisturizing effects, and is convenient for operation and feeding.

[0040] The composite antibiotics and composite preservatives used in the present invention can prolong the shelf life of feed, prevent feed corruption and deterioration, and strictly control the amount of addition to reduce the impact on larval growth. High temperature and high pressure sterilization is used in the process of feed preparation to reduce the impact of microorganisms in the environment on the feeding effect. Compared with natural feed, it has better anti-mildew effect and is more convenient to use.

[0041] The invention adopts a combination of ascorbic acid, a mixed solution of complex vitamin B and cholesterol, linolenic acid and linoleic acid to provide vitamins and trace substances required for the growth and development of tomato leafminer larvae.

[0042] The raw materials used in the present invention are all products that are easily purchased, and the content of nutritional components in the products is controllable.

[0043] In summary, the artificial feed for tomato leafminer larvae provided by the present invention has comprehensive nutrition, reasonable proportion, stable feeding effect, strong practicality, can enable the tomato leafminer to complete the entire pre-adult growth and development, and has high practical value and promotion value in the field of insect artificial breeding technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 : Larvae of tomato leafminer feeding on artificial diet.

[0045] Figure 2 : Pupae fed on artificial diet of tomato leafminer. DETAILED DESCRIPTION

[0046] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0047] Unless otherwise specified, the materials, reagents, instruments, etc. used in the following examples can be obtained from commercial sources.

[0048] Example 1: Preparation of artificial feed for tomato leafminer

[0049] An artificial feed for tomato leafminer, the raw material composition and weight ratio of the artificial feed are:

[0050] (1) Cellulose 50 parts, lactic acid casein 15 parts, wheat germ powder 15 parts, Wesson salt 3 parts, soybean powder 5 parts, agar 14 parts, sucrose 10 parts, glucose 2 parts, cholesterol 0.5 parts, linolenic acid 1.0 parts, complex preservative solution 10 parts, complex antibiotic solution 1 part, linoleic acid 1 part, soybean oil 2 parts, complex vitamin B solution 10 parts, tomato homogenate 200 parts, distilled water 400 parts.

[0051] (2) Cellulose 40 parts, lactic acid casein 12 parts, wheat germ powder 12 parts, Wesson salt 3 parts, soybean powder 4 parts, agar 10 parts, sucrose 10 parts, glucose 1 part, cholesterol 0.4 parts, linolenic acid 0.8 parts, complex preservative solution 5 parts, complex antibiotic solution 0.5 parts, linoleic acid 0.8 parts, soybean oil 1 part, complex vitamin B solution 8 parts, tomato homogenate 150 parts, distilled water 350 parts.

[0052] (3) Cellulose 55 parts, lactic acid casein 18 parts, wheat germ powder 18 parts, Wesson salt 4 parts, soybean powder 6 parts, agar 15 parts, sucrose 15 parts, glucose 2 parts, cholesterol 0.5 parts, linolenic acid 1.2 parts, complex preservative solution 10 parts, complex antibiotic solution 2 parts, linoleic acid 1.2 parts, soybean oil 2 parts, complex vitamin B solution 10 parts, tomato homogenate 150 parts, distilled water 500 parts.

[0053] The vitamin B complex solution is prepared by dissolving the following components in proportion, and the weight parts of the vitamin B complex solution include: 5 parts of riboflavin, 2.5 parts of lutein, 5 parts of 1 mol / L KOH solution, 1 part of niacinamide, 1 part of calcium pantothenate, 2.5 parts of thiamine hydrochloride, 2.5 parts of pyridoxine hydrochloride, 0.2 parts of biotin, vitamin B 12 0.2 parts, 950 parts of distilled water. The preparation method comprises the following steps: weigh the above components with a 1 / 10,000 balance, put them into a container, add 950 parts of sterilized distilled water, mix them thoroughly, and put them into a refrigerator at 4 degrees Celsius for standby use.

[0054] The compound antibiotic solution is prepared from the following raw materials in parts by weight: 5 parts of tetracycline, 3 parts of penicillin, 2 parts of metronidazole, and 100 parts of distilled water. The weight ratios are accurately weighed with an electronic balance, fully mixed, and divided for use.

[0055] The composite preservative is prepared from the following raw materials in parts by weight: 10 parts of methyl parahydroxybenzoate, 5 parts of propionic acid, 5 parts of sodium acetate, and 100 parts of 75% ethanol solution. The weight ratios are accurately weighed with an electronic balance, fully mixed, and divided for use.

[0056] Taking the formula in (1) as an example, the method for preparing the artificial feed for tomato leafminer larvae comprises the following steps:

[0057] S1. Put 100 parts of tomatoes, 100 parts of purified water, and 0.5 parts of vitamin C into a juicer and run for 1-2 minutes to obtain a host plant homogenate, and proceed to step S2;

[0058] S2. Add 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 3 parts of Wesson salt, 5 parts of soybean flour, 14 parts of agar, 10 parts of sucrose, 2 parts of glucose, 0.5 parts of cholesterol, 10 parts of composite preservative solution, and 200 parts of host plant homogenate to 400 parts of distilled water, stir evenly, place it in a sterilizer, sterilize it at 105 degrees Celsius for 15-20 minutes, and enter step S3;

[0059] S3. When the temperature of the sterilized mixture in S2 drops to 60°C, add 10 parts of complex vitamin B solution, 1 part of complex antibiotic solution, 1 part of linolenic acid, 1 part of linoleic acid, and 2 parts of soybean oil, stir evenly, pour it into a sterile container and cool it until solidified to obtain artificial feed for tomato leafminer larvae. Place it in an ultra-clean workbench for 4-5 hours to evaporate excess moisture, and finally seal it and place it in a 4°C refrigerator for use.

[0060] Example 2: Application of artificial diet for tomato leafminer

[0061] In order to better verify the practical application effect of the present invention, the following experiments are conducted to compare the development index data of artificial feed for tomato leafminer larvae, natural feed for tomato leafminer - tomato leaves, and artificial feed for the closely related species potato tuber moth, and to compare the growth and development of the larvae under different feeding methods.

[0062] The experimental method is: the newly hatched larvae of tomato leafminer are placed in culture containers filled with artificial feed and natural feed (tomato leaves) respectively, the feed is replaced when it deteriorates or shriveles, and the growth and development are recorded.

[0063] The specific steps are as follows:

[0064] (1) Cut the artificial diet or natural diet of tomato leafminer larvae into small pieces of 1.0×0.5×0.5 cm and place them in a 12-well plate with a hole diameter of 2 cm, or cut it into small pieces of 1.5×1.5×1.5 cm and place them in a 6-well plate with a hole diameter of 4 cm, with one piece placed in each well;

[0065] (2) Place newly hatched larvae in any well of a 6-well or 12-well plate, cover with a layer of absorbent paper, cover the well plate and tie it tightly with a rubber band;

[0066] (3) Place the 6-well or 12-well plate in an incubator and maintain the culture environment at 27±0.5℃, a photoperiod of 12L:12D, and a humidity of 35±5%.

[0067] The artificial diet for potato tuber moth larvae includes: 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 3 parts of Wesson salt, 5 parts of soybean powder, 8 parts of agar, 10 parts of sucrose, 2 parts of glucose, 0.5 parts of cholesterol, 1 part of methyl paraben, 1 part of linoleic acid, 2 parts of soybean oil, 10 parts of vitamin B complex solution, 200 parts of potato homogenate, and 400 parts of distilled water.

[0068] The complex vitamin B solution is prepared by dissolving vitamin B1-12 in proportion to prepare the complex vitamin B solution.

[0069] The weight parts include: 1000 parts of vitamin B4, 200 parts of vitamin B8, 10 parts of vitamin B3, 10 parts of vitamin B5, 5 parts of vitamin B2, 2.5 parts of vitamin B1, 2.5 parts of vitamin B6, 2.5 parts of vitamin B9, 0.2 parts of vitamin B7, 0.2 parts of vitamin B12, and 4000 parts of distilled water. The preparation method of the complex vitamin B solution includes the following steps: weighing the above components with a 1 / 10,000 balance, putting them into a container, adding 4000 parts of sterilized distilled water, mixing them thoroughly, and putting them into a refrigerator at 4 degrees Celsius for standby use.

[0070] The percentage composition of Wesson salt is: CaCO3: 21%, CuSO4·5H2O: 0.039%, Fe3(PO4)2·H2O:

[0071] 1.470%, MnSO4: 0.020%, MgSO4: 9.000%, K2Al(SO4)4·2H2O: 0.009%, KCl: 12.000%, KH2PO4: 31.000%, KI: 0.005%, NaC1: 10.500%, NaF: 0.057%, Ca3(PO4)2: 14.900%. The above ingredients are crushed and passed through an 80-mesh sieve, accurately weighed with an electronic balance according to the mass ratio, fully mixed, and divided for use.

[0072] The preparation method of artificial feed for potato tuber moth larvae comprises the following steps:

[0073] S1, add 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 3 parts of Wesson salt, 5 parts of soybean flour, 14 parts of agar, 10 parts of sucrose, 2 parts of glucose, 0.5 parts of cholesterol, 11 parts of methyl parahydroxybenzoate solution (1 part of methyl parahydroxybenzoate is dissolved in 10 parts of anhydrous ethanol), 1 part of linoleic acid, and 2 parts of soybean oil to 400 parts of distilled water, stir evenly, place it in a sterilizer, sterilize it at 115 degrees Celsius for 15-20 minutes, and enter step S2;

[0074] S2, peeling potato tubers and cutting them into small pieces, soaking 100 parts of potato pieces in 100 parts of 0.3% vitamin C solution for 10-30 minutes in advance, putting them into a juicer for 1-2 minutes to obtain potato homogenate, and entering step S3;

[0075] S3, add the potato slurry in S2 to the sterilized mixture in step S1, stir evenly, wait until the temperature drops to about 60 degrees Celsius, add 10 parts of complex vitamin B solution successively, continue stirring for about 2 minutes to fully mix the feed, then pour it into a sterilized culture dish and cool it until it solidifies to obtain artificial feed for potato tuber moth larvae, place it on an ultra-clean workbench for 4-5 hours to evaporate excess water, and finally seal it and place it in a 4 degree Celsius refrigerator for standby use.

[0076] The other steps (rearing methods) are the same as those for the artificial diet and natural diet for tomato leafminer larvae.

[0077] The development period, adult lifespan and egg-laying amount of each insect stage are shown in Table 1 below, and the survival rate of each insect stage of tomato leafminer feeding on different foods is shown in Table 2.

[0078] Table 1 Developmental periods of eggs, larvae and pupae of tomato leafminer fed on different foods

[0079]

[0080] Table 2 Survival rates of different stages of tomato leafminer feeding on different foods

[0081]

[0082] The results show that the developmental period of each insect stage of the artificial feed of the tomato leafminer in the present invention is shorter than that of the artificial feed of the potato tuber moth, and the egg-laying amount is significantly higher than that of the artificial feed of the potato tuber moth. The artificial feed of the present invention is basically similar to most indicators of the natural host - tomato leaves, and is higher than tomato leaves in terms of adult emergence rate. The artificial feed prepared by the formula of the present invention feeds tomato leafminer larvae, and its larval development period, larval survival rate, pupal period, and adult life span are comparable to those of natural host plants, but in terms of time cost and feeding efficiency, artificial feed has more advantages. In addition, during the test, the artificial feed of the embodiment of the present invention takes more than 4-5 days from feeding to the appearance of mildew, while the detached tomato leaves will shrive within 24-36 hours, which proves that the tomato leafminer feed of the present invention has antibacterial and mildew-proof effects and can achieve long-term feeding of tomato leafminers. The pictures of the tomato leafminer larvae and pupae raised in this experiment are as follows Figure 1 and Figure 2 shown.

[0083] Example 3: Application of artificial diet for tomato leafminer

[0084] In order to verify the necessity of adding linolenic acid, the following two artificial feeds were compared with other related indicators such as mating efficiency, egg laying volume and F1 generation survival rate.

[0085] The ingredients of feed A include: 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 2 parts of Wesson salt, 5 parts of soybean powder, 10 parts of agar, 10 parts of sucrose, 1 part of glucose, 0.5 parts of cholesterol, 1.0 parts of linolenic acid, 8 parts of complex preservative solution, 1 part of complex antibiotic solution, 1 part of linoleic acid, 2 parts of soybean oil, 10 parts of complex vitamin B solution, 200 parts of tomato homogenate, and 400 parts of distilled water.

[0086] Feed B ingredients include: 50 parts of cellulose, 15 parts of lactic acid casein, 15 parts of wheat germ powder, 2 parts of Wesson salt, 5 parts of soybean powder, 10 parts of agar, 10 parts of sucrose, 1 part of glucose, 0.5 parts of cholesterol, 8 parts of complex preservative solution, 1 part of complex antibiotic solution, 1 part of linoleic acid, 2 parts of soybean oil, 10 parts of complex vitamin B solution, 200 parts of tomato homogenate, and 400 parts of distilled water.

[0087] The pupae fed with the two feeds were separated into male and female under a dissecting microscope and placed in 1.5 mL centrifuge tubes. After they emerged as adults, one female and one male were placed in a 9 cm diameter culture dish to observe the frequency and duration of mating for 48 hours. The egg production and hatching rate of females were also counted.

[0088] The statistical results are shown in the following table

[0089] Table 3 Mating efficiency, egg laying and egg hatching rate of tomato leafminer fed with different diets

[0090]

[0091] Linolenic acid is a key precursor for the synthesis of sex pheromones of tomato leafminers. Adding a trace amount of linolenic acid to the artificial feed is beneficial to the synthesis of sex pheromones of the insect, which helps the mating of male and female insects, thereby improving the reproduction efficiency. The results show that the linolenic acid added in the present invention can significantly increase the first-day mating ratio, mating duration and egg-laying amount of tomato leafminers. This shows that linolenic acid has also improved the reproductive capacity of the insect.

[0092] Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person skilled in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, and these changes or substitutions shall be within the scope of protection of the present invention.

Claims

1. A feed for tomato leafminer, characterized in that: The feed comprises, by weight, 40-55 parts of cellulose, 12-18 parts of lactic acid casein, 12-18 parts of wheat germ powder, 3-4 parts of Wesson salt, 4-6 parts of soybean powder, 10-15 parts of agar, 10-15 parts of sucrose, 1-2 parts of glucose, 0.4-0.5 parts of cholesterol, 0.8-1.2 parts of linolenic acid, 0.8-1.2 parts of linoleic acid, 1-2 parts of soybean oil, 8-10 parts of complex vitamin B solution, 150-200 parts of host plant homogenate, 0.5-2 parts of complex antibiotic solution, 5-10 parts of complex preservative solution and 350-500 parts of distilled water. The complex vitamin B solution comprises, by weight, 4-6 parts of riboflavin, 1.5-3.5 parts of lutein, 1 4-6 parts of mol / L KOH solution, 0.5-2.0 parts of nicotinamide, 0.5-2.0 parts of calcium pantothenate, 1.5-3.5 parts of thiamine hydrochloride, 1.5-3.5 parts of pyridoxine hydrochloride, 0.1-0.5 parts of biotin, vitamin B 12 0.1-0.5 parts, distilled water 500-1000 parts.

2. The tomato leafminer feed according to claim 1, characterized in that The compound antibiotic solution comprises tetracycline, penicillin, metronidazole and distilled water.

3. The tomato leafminer feed according to claim 1, characterized in that The composite preservative comprises methyl parahydroxybenzoate, propionic acid, sodium acetate and 75% ethanol solution.

4. The tomato leafminer feed according to claim 1, characterized in that The host plant homogenate is obtained by squeezing juice from host plants, vitamin C and pure water, and the host plant includes tomatoes.

5. The method for preparing the tomato leafminer feed according to any one of claims 1 to 4, characterized in that: The method comprises mixing the components of the tomato leafminer feed.

6. The preparation method according to claim 5, characterized in that: The preparation method comprises: S1. Put the host plant, purified water and vitamin C into a juicer to obtain a host plant homogenate; S2. Homogenize cellulose, lactic acid casein, wheat germ powder, Wesson salt, soybean powder, agar, sucrose, glucose, cholesterol, composite preservative solution and host plant, add to distilled water, stir evenly, and sterilize; S3. After the sterilized mixture in S2 is cooled, add the complex vitamin B solution, the complex antibiotic solution, linolenic acid, linoleic acid and soybean oil, stir evenly, cool until condensed, obtain the tomato leafminer feed, and evaporate excess water.

7. A method for cultivating tomato leafminer, characterized in that: The method comprises cultivating tomato leafminers using the tomato leafminer feed described in any one of claims 1 to 4 or the tomato leafminer feed obtained by the preparation method described in any one of claims 5 to 6.

8. The culture method according to claim 7, characterized in that: The culture method comprises: culturing newly hatched larvae of tomato leafminer in feed.

Images