Method for producing ultrathin fermentation bed bacteria
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A fermented bed, ultra-thin technology, applied in the field of microbial fermentation, can solve the problems of low microbial content of fermented bed bacteria, unreasonable combination of microbial varieties, and poor adaptability of microorganisms, etc., to achieve reasonable variety, low cost, and adaptability strong effect
Inactive Publication Date: 2011-06-15
HUBEI QIMING BIO ENG
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Problems solved by technology
However, the currently promoted fermented bed pig raising technology is generally a thick-layer wet fermented bed, and the thickness of the fermented bedding material is generally 60-100 cm, and the thinnest fermented bedding material is also more than 40 cm. Larger transformation of the pigsty requires a lot of litter and high cost, and the thicker litter contains more water, which will cause the microorganisms to ferment and make the fermentation bed temperature too high, which will affect the performance of pigs on the bedding. comfort of living
This is mainly due to the low microbial content of the fermented bed bacteria used in the thick fermented bed, unreasonable combination of microbial species, and weak adaptability of microorganisms.
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Embodiment example 1
[0072] 1. Strain preparation:
[0073] 1. Transfer the seeds of Rhizopus oryzae, Aspergillus niger, and Candida utilis preserved in sand tubes to Potato Dextrose Agar (PDA) slant medium, and culture them at 30°C for 2 days. in vivo and then transferred to bran medium. Bacillus subtilis, Bacillus subtilis, and Enterococcus faecalis were transferred to nutrient agar medium and cultured at 37°C for 24 hours for later use.
[0074] 2. The production of triangular bottle seeds:
[0075] 1) Mix bran and sawdust at a ratio of 1:1, add trace elements (based on the mass of compost) magnesium sulfate 1.5%, potassium dihydrogen phosphate 1.5%, calcium carbonate 1.5%, add water to make the compost moisture content at 60 ﹪, divide them into triangular flasks, fill each bottle with a culture material about 2cm thick, put a cotton plug in it, put it in a sterilizing pot, sterilize it at 121°C for 30 minutes, shake it while it is hot, and cool it to below 30°C spare. Under aseptic operati...
Embodiment example 2
[0090] 1. Strain preparation:
[0091] 1. Transfer the seeds of Rhizopus oryzae, Aspergillus niger, and Candida utilis preserved in sand tubes to Potato Dextrose Agar (PDA) slant medium, and culture them at 27°C for 3 days. in vivo and then transferred to bran medium. Bacillus subtilis, Bacillus subtilis, and Enterococcus faecalis were transferred to nutrient agar slant medium, and cultured at 37°C for 24 hours for later use.
[0092] 2. The production of triangular bottle seeds:
[0093] 1) Mix bran and sawdust in a ratio of 1:1, add trace elements (based on the mass of culture material) magnesium sulfate 1%, potassium dihydrogen phosphate 1.5%, calcium carbonate 1%, so that the water content is 55% , divided into triangular flasks, each bottle filled with compost about 4CM thick, plugged with a cotton plug, put into a sterilizing pot, sterilized at 121°C for 40 minutes, shaken while hot, cooled to below 30°C for later use . Under aseptic operation, use the prepared bran ...
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Abstract
The invention discloses a method for producing ultrathin fermentation bed bacteria, and belongs to the field of microorganism fermentation. Adopted strains are rhizopus oryzae, aspergillus niger, candida utilis, bacillus licheniformis, bacillus subtilis and enterococcus faecium; and the method comprises the following steps of: performing single pure culture on the strains to obtain strains for producing; mixing the strains uniformly, inoculating into a koji plate for culture and fermentation, and fermenting for 2 to 3 days at the pH value of 6.5-7.5; and drying, crushing and packaging to obtain the ultrathin fermentation bed bacteria. The ultrathin fermentation bed bacteria have high viable count of microbes, total viable count of fungi of more than 0.5 billion / gram and the total viable count of bacteria of 4.5 billion / gram, reasonable in type collocation, and high in adaptivity; and when the ultrathin fermentation bed bacteria are matched with an ultrathin fermentation bed technology, old pig pens are not needed to be transformed basically, the cost of newly-built pig pens is obviously reduced, the using amount of a padding for building the fermentation bed is small, the cost is low, the temperature of the fermentation bed can be controlled, the fermentation bed is warm in winter and cool in summer, the problem that the conventional fermentation bed is humid and hot in summer is obviously solved, and the used padding for the fermentation bed is a good-quality microbial organic fertilizer.
Description
technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a production method of an ultra-thin fermentation bed microbial agent for ecological cultivation. Background technique [0002] Fermentation bed pig raising is an ecological pig raising method developed in recent years. It effectively solves the defects of traditional pig raising such as environmental pollution, high labor intensity, and many diseases, and the fermented mattress material after use is high-quality biological Organic fertilizers, thus solving a series of problems caused by the large-scale application of chemical fertilizers. However, the currently promoted fermented bed pig raising technology is generally a thick-layer wet fermented bed, and the thickness of the fermented bedding material is generally 60-100 cm, and the thinnest fermented bedding material is also more than 40 cm. Larger transformation of the pigsty requires a lot of litter and hig...
Claims
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Application Information
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