Method for producing ultrathin fermentation bed bacteria
A fermented bed, ultra-thin technology, applied in the field of microbial fermentation, can solve the problems of low microbial content of fermented bed bacteria, unreasonable combination of microbial varieties, and poor adaptability of microorganisms, etc., to achieve reasonable variety, low cost, and adaptability strong effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment example 1
[0072] 1. Strain preparation:
[0073] 1. Transfer the seeds of Rhizopus oryzae, Aspergillus niger, and Candida utilis preserved in sand tubes to Potato Dextrose Agar (PDA) slant medium, and culture them at 30°C for 2 days. in vivo and then transferred to bran medium. Bacillus subtilis, Bacillus subtilis, and Enterococcus faecalis were transferred to nutrient agar medium and cultured at 37°C for 24 hours for later use.
[0074] 2. The production of triangular bottle seeds:
[0075] 1) Mix bran and sawdust at a ratio of 1:1, add trace elements (based on the mass of compost) magnesium sulfate 1.5%, potassium dihydrogen phosphate 1.5%, calcium carbonate 1.5%, add water to make the compost moisture content at 60 ﹪, divide them into triangular flasks, fill each bottle with a culture material about 2cm thick, put a cotton plug in it, put it in a sterilizing pot, sterilize it at 121°C for 30 minutes, shake it while it is hot, and cool it to below 30°C spare. Under aseptic operati...
Embodiment example 2
[0090] 1. Strain preparation:
[0091] 1. Transfer the seeds of Rhizopus oryzae, Aspergillus niger, and Candida utilis preserved in sand tubes to Potato Dextrose Agar (PDA) slant medium, and culture them at 27°C for 3 days. in vivo and then transferred to bran medium. Bacillus subtilis, Bacillus subtilis, and Enterococcus faecalis were transferred to nutrient agar slant medium, and cultured at 37°C for 24 hours for later use.
[0092] 2. The production of triangular bottle seeds:
[0093] 1) Mix bran and sawdust in a ratio of 1:1, add trace elements (based on the mass of culture material) magnesium sulfate 1%, potassium dihydrogen phosphate 1.5%, calcium carbonate 1%, so that the water content is 55% , divided into triangular flasks, each bottle filled with compost about 4CM thick, plugged with a cotton plug, put into a sterilizing pot, sterilized at 121°C for 40 minutes, shaken while hot, cooled to below 30°C for later use . Under aseptic operation, use the prepared bran ...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com