The invention provides biological organic nutrient soil, which comprises, by weight, 80-100 parts of coconut residuum, 40-50 parts of peat soil, 10-20 parts of perlite, 5-10 parts of vermiculite, 20-30 parts of a decomposed biological organic fertilizer, 5-10 parts of attapulgite, 4-6 parts of tobacco extract, 0.1-0.3 part of tea saponin, and 4-6 parts of a composite microbial agent. The coconut residuum and peat soil are added into the nutrient soil, so that the nutrient soil has good air permeability and water retention property; the biological organic fertilizer is added to serve as a nutrient source in the nutrient soil, the nutrient elements are comprehensive, the fertilizer efficiency lasts long, and nutrients can be continuously provided for seedlings. The biological organic nutrient soil is prepared by mixing pure natural materials, the prepared nutrient soil is environmentally friendly and nontoxic, and the raw materials used in the formula and the preparation method are simple, and the nutrient soil is convenient to use and free of peculiar smell; the biological organic nutrient soil is good in soil structure, good in air permeability, good in water and fertilizer retention performance and good for growth and development of seedlings.