Method of using corn distilled grains and waste bean dregs to ferment and produce animal feed

A technology of corn distillers grains and animal feed, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problems such as probiotics that have not yet appeared, and achieve the effects of enhancing animal immunity, increasing amino acid content, and improving intestinal environment.

Active Publication Date: 2019-05-17
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although corn distillers grains and okara have their own treatment methods, there is no precedent for mixing the two together with probiotics for fermentation in the current report

Method used

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  • Method of using corn distilled grains and waste bean dregs to ferment and produce animal feed
  • Method of using corn distilled grains and waste bean dregs to ferment and produce animal feed
  • Method of using corn distilled grains and waste bean dregs to ferment and produce animal feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this example, the inoculation ratios of Bacillus subtilis and Clostridium butyricum were investigated, and the seed liquids of Bacillus subtilis and Clostridium butyricum in different proportions were inserted into corn distiller's grains according to the inoculation amount of 5% (V / W). Put it in a solid fermentation medium with a ratio of 2:3 to bean dregs, put it in a fermenter, and ferment at a temperature of 35°C. First, ferment aerobically for 24 hours, and then pass in an air volume of 0.5 (V / V m), and then ferment anaerobically for 48 hours. , the amount of nitrogen gas introduced is 0.5 (V / V·m). The ratio of Bacillus subtilis: Clostridium butyricum was 1:0, 0:1, 1:1, 1:2, 2:1, 2:3, 3:2, and the total colony number and crude protein ( dry basis), crude fat (dry basis), crude fiber (dry basis), the results are shown in Table 1. As known from Table 1, Bacillus subtilis plays a key role in the total number of colonies, and the number of colonies with Bacillus su...

Embodiment 2

[0051] The present invention investigates the ratio of corn distiller's grains and bean dregs in the solid fermentation medium. The liquid seed solution with a ratio of 1:2 of Bacillus subtilis and Clostridium butyricum is added according to the inoculation amount of 5% (V / W). Proportionate solid fermentation medium, placed in a fermenter at a fermentation temperature of 35°C, aerobic fermentation for 24 hours, air flow of 0.5 (V / V m), and anaerobic fermentation for 48 hours, nitrogen flow of 0.5 (V / V·m). The ratios of corn distiller's grains and bean dregs are 0:1, 1:0, 1:1, 1:2, 1:3, 2:1, 2:3 respectively, and the total number of colonies and crude protein (dry basis) of the fermentation products are determined ), crude fat (dry basis), crude fiber (dry basis), the results are shown in Table 2. As can be seen from Table 2, in the experiment of corn distiller's grains or bean dregs alone, the physical and chemical indicators of the fermentation products are very low. When th...

Embodiment 3

[0055] The present invention investigates the solid-state fermentation time, the ratio of Bacillus subtilis and Clostridium butyricum is 1:2 liquid seed solution, according to 5% (V / W) inoculum amount, the ratio of adding corn distiller's grains and okara is 2: In the solid medium of 3, the fermentation temperature is 35°C, the air volume is 0.5 (V / V m) during aerobic fermentation, and the nitrogen volume is 0.5 (V / V m) during anaerobic fermentation, and the fermentation time is respectively A: Early aerobic 0h, late anaerobic 72h; B: Early aerobic 12h, late anaerobic 60h; C: Early aerobic 24h, late anaerobic 48h; D: Early aerobic 36h, late anaerobic 36h; E: Aerobic 48h in the early stage, anaerobic 24h in the late stage. The total number of colonies, crude protein (dry basis), crude fat (dry basis) and crude fiber (dry basis) of the fermentation product were measured, and the results are shown in Table 3. As can be seen from Table 3, the number of colonies in a completely ae...

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Abstract

The invention discloses a method of using corn distilled grains and waste bean dregs to ferment and produce animal feed. The method comprises the steps of smashing and drying the corn distilled grainsand the waste bean dregs to prepare a solid fermentation culture medium; conducting liquid fermentation on bacillus subtilis strains and clostridium butyricum strains separately, mixing and proportioning the bacillus subtilis strains and the clostridium butyricum strains according to the volume ratio of (1-3):(1-3), inoculating the mixed and proportioned liquid strains into the solid fermentationculture medium according to the ratio of 2-10% (V / W), placing the mixture into a fermentation tank at the fermentation temperature of 25-50 DEG C, conducting aerobic fermentation for 12-24 hours first with inject air amount of 0.2-1 (V / V m), then, conducting anaerobic fermentation for 24-60 hours with inject nitrogen amount of 0.2-1 (V / V m), after fermentation, drying fermentation products at lowtemperature, and obtaining the animal feed. According to the animal feed, the maximum total viable count can be 3.8*1011 cfu / g, the crude protein content reaches up to 68%, and the amino acid contentis obviously increased; the animal feed exceeds the national feed standard, is more conveniently absorbed by livestock intestinal tracts, improves the intestinal tract environments of livestock, improves the immunity of animals, and has an obvious effect of promoting growth of the animals.

Description

technical field [0001] The invention belongs to the technical field of animal feed preparation, and in particular relates to a method for producing animal feed by fermenting corn distiller's grains and waste bean dregs. Background technique [0002] In the process of producing ethanol by fermentation with corn as raw material, the starch in it is converted into ethanol and carbon dioxide, and other nutrients such as protein, fat, fiber, etc. are left in the distiller's grains. At the same time, due to the action of microorganisms, the content of protein, B vitamins and amino acids in distiller's grains is higher than that of corn, and contains unknown growth-promoting factors produced during fermentation. There are two types of corn distiller's grain protein feed products on the market: one is DDG, which is obtained by simply filtering corn distiller's grains, drying the filter residue, draining the filtrate, and drying the filter residue alone; the other is DDGS , is the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/18A23K10/37A23K10/38
CPCY02P60/87
Inventor 郝宁刘桢琦周勇卢宗梅郭格格陈影郭世堂陈可泉
Owner NANJING UNIV OF TECH
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