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Production method of large-scale rice wheat straw microorganism feed

A microbial feed, rice and wheat straw technology, applied in the field of new microbial roughage, can solve problems such as waste, limited lignin degradation, and large loss of bacterial enzyme activity

Active Publication Date: 2016-09-07
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter uses volatile acid for pretreatment. If it is produced on a large scale, the natural volatilization of acid to remove acid will cause air pollution and is also very wasteful. Moreover, dilute acid treatment is mainly to dissolve hemicellulose, and it hardly dissolves lignin. , will naturally affect the effect of Neurospora crassa on degrading rice straw. In addition, only Neurospora crassa degrades rice straw, and the lignin enzyme system is incomplete, so the degradation of lignin is limited, which seriously affects the feed utilization rate.
[0010] In general, the above-mentioned fermentation products are all dried, resulting in a large loss of bacterial enzyme activity, high energy consumption, poor palatability, and no optimization and integration of follow-up processing

Method used

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  • Production method of large-scale rice wheat straw microorganism feed
  • Production method of large-scale rice wheat straw microorganism feed
  • Production method of large-scale rice wheat straw microorganism feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: pre-production of white rot fungus enzyme preparation

[0075] (1) White rot fungus culture medium.

[0076] Slant medium and plate medium: 200g potato, 20g sucrose, 20g agar, in 1L constant volume, pH 5.0, sterilized at 110°C for 30min. Inoculate and culture at 37°C for 4 days.

[0077] Seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort juice or glucose 3g, ammonium tartrate 1g, dilute to 1L, in which FeSO 4 ·7H 2 O, ZnSO 4 、CuSO 4 ·5H 2 O, MnSO 4 .4H 2 O and CoCl 2 0.001% each, pH 5.5. Add 100ml medium to a 500ml Erlenmeyer flask, and sterilize at 110°C for 30min.

[0078] White rot fungus solid-state fermentation seed culture medium: by weight, cut, shredded and crushed rice and wheat (below 20mm) account for 70% of the medium (calculated as dry material), bran or rice bran accounts for 25%, tartrate amine , ammonium nitrate, ammonium sulfate or ammonium chloride accounted for 0.2%, CaCO 3 1%...

Embodiment 2

[0081] Embodiment 2: the first phase of rice straw fermentation

[0082] (1) Seed medium and production medium of fiber-degrading bacteria such as Neurospora.

[0083] Neurospora and other slant medium and plate medium: 200g potato, 20g sucrose, 20g agar, constant volume 1L, pH 5.0, sterilized at 110°C for 30min. Inoculate and culture at 32°C for 2 days.

[0084] Neurospora and other seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort juice or brown sugar 3g, ammonium sulfate or urea 5g, FeSO 4 ·7H 2 O 0.005g, ZnSO 4 0.0014g, MnSO 4 4H 2 O 0.0016g, CoCl 2 0.002g, dilute to 1L, pH 5.0. Add 100ml medium to a 500ml Erlenmeyer flask, and sterilize at 110°C for 30min.

[0085] Candida utilis slant culture medium and plate culture medium (100ml): glucose 2g, yeast extract 1g, peptone 1g, urea 1g, agar 2g, pH value about 6.0.

[0086] Candida utilis liquid medium: the composition is the same as that of the slant medium, witho...

Embodiment 3

[0093] Embodiment 3: the second phase of rice straw fermentation

[0094] (1) Bacillus culture medium

[0095] Bacillus slant and plate culture medium: peptone 10g, beef extract powder 5g, sodium chloride 5g, agar 15g, glucose 20g, distilled water 1000ml, final pH7.0±0.2. Sterilize at 121°C for 20min.

[0096] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl 2 ·6H 2 O 0.07g / L, MnCl 2 ·7H 2 O 0.01g / L, MgSO 4 ·7H 2 O 0.15g / L, pH 6.5-7.0, sterilized at 110-121°C for 20min.

[0097] (2) Saccharomyces cerevisiae medium

[0098] Yeast slant medium and plate culture medium (100ml): 2g glucose, 1g yeast extract, 2g peptone, 2g agar, pH value about 6.0.

[0099] Shake flask seed medium and liquid seed tank medium: the composition is the same as that of the slant medium, without adding agar.

[0100] (3) The second-stage rice-wheat straw solid-state fermentation medium is composed of the first-stage fermentation p...

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Abstract

The invention discloses a production method of a large-scale rice wheat straw microorganism feed, and belongs to the field of biological feeds. Rice wheat straw is used as a main carbon source, and inorganic nitrogen is used as a main nitrogen source. The production method comprises the following steps of performing high-efficient pure solid cultivation on a white rot fungus enzyme preparation, then converting the cultivated white rot fungus enzyme preparation in large inoculum amount to a first-stage fermentation medium of the rice wheat straw, and besides, inoculating serial lignocelluloses degradation bacteria and candida utilis bacteria for synergistic rapid degradation and transformation; adding bacillus and saccharomyces cerevisiae for second-stage fermentation; and using lactic acid bacteria for third-stage anaerobic fermentation so as to obtain finished products. The lignin of the straw biological feed is greatly reduced, genuine protein is increased to 11%b or above, and the raw materials do not need to be sterilized; the finished products do not need drying treatment, lysozymes are enriched, and the production cost is extremely low. The large-scale rice wheat straw microorganism feed can be used as a superior coarse feed and a protein grain feed for cattle and sheep, and the additive quantity can reach 60% or above; the additive quantity can also be about 10%, so that the large-scale rice wheat straw microorganism feed is used for simple stomach animals. The large-scale rice wheat straw microorganism feed is suitable for mass production, and the feeding and economic effects are obvious.

Description

technical field [0001] The invention belongs to the field of biological feed, and relates to a large-scale production method of rice straw microbial feed, in particular to a novel microbial roughage with high efficiency and low cost based on rice straw and wheat straw as raw materials. Background technique [0002] In the environment where grazing is prohibited on grasslands and house feeding is advocated, roughage is an essential raw material for ruminants such as cattle and sheep. In particular, a bottleneck in cattle and sheep breeding in the south is the lack of high-quality roughage. The effect of planting alfalfa and other forages is poor, and it occupies land The high cost of transporting forage also restricts the development of the dairy industry in the Yangtze River Delta. However, there are a lot of rice straw in the south, and there are also a lot of wheat straw, which is an important source of roughage. However, the texture of rice and wheat straw is rough and har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K20/158A23K20/26A23K50/10A23K10/14
CPCY02P60/87
Inventor 陈华友贾金如李萍萍杨胜利谢永明吴邦国乐易林崔凤杰倪忠
Owner JIANGSU UNIV
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