Composite inoculant for preventing and treating cucumber root knot nematode disease and preparation method thereof

A technology of root-knot nematode disease and combined inoculants, applied in nematicides, botanical equipment and methods, biocides, etc., can solve the problems of AM fungi and PGPR biocontrol potential and effect yet to be evaluated, and achieve nematode drug resistance The effect of enhancing, promoting the growth of cucumber and reducing environmental pollution

Inactive Publication Date: 2015-05-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of vegetable root-knot nematode control, the biocontrol potential and effect of AM fungi and PGPR under certain conditions need to be evaluated

Method used

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  • Composite inoculant for preventing and treating cucumber root knot nematode disease and preparation method thereof
  • Composite inoculant for preventing and treating cucumber root knot nematode disease and preparation method thereof
  • Composite inoculant for preventing and treating cucumber root knot nematode disease and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0017] (1) Glomus mosseae (Glomus mosseae), represented by Gm in the text, is propagated by clover in sterilized sandy soil for 8-10 months, with spores, mycorrhizal root segments, mycelium in soil and rhizosphere The inoculum mixture composed of soil is ready for use; Bacillus subtilis (Bacillus subtilis), represented by BS in the text, is transferred to beef extract peptone liquid medium, shaken and cultured at 29°C for 30 hours, and the bacterial suspension is diluted to a concentration of 3×10 8 CFU / ml spare.

[0018] (3) inoculate Glomus mosei during sowing, the inoculation amount is 5000 inoculation potentials of each cucumber; when the cucumber grows to three leaves and one heart, inoculate Bacillus subtilis with the root irrigation method, and the inoculum amount is 3 × 10 inoculation concentration of each cucumber. 8 CFU / ml Bacillus subtilis 10ml.

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Abstract

The invention belongs to the field of biological prevention and treatment, and provides antibiological inoculant for preventing and treating cucumber root knot nematode disease. The composite microbial inoculant is prepared from glomus mosseae inoculums and bacillus subtilis fermentation broth; the composite inoculants is selected from nine simple inoculants and twelve composite inoculants according to the development condition and nematode resistance condition; each composite microbial inoculant includes 5000 inoculum potential glomus mosseae; the total viable counts of bacillus subtilis fermentation broth reach 3*10<8>CFU/ml; glomus mosseae and bacillus subtilis inoculants are compounded and show obvious a synergistic interaction effect on preventing and treating root knot nematode by being compared with individual glomus mosseae inoculants or bacillus subtilis inoculants, and the preventing effect of root knot nematode is also obviously improved.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a combined microbial agent for preventing and treating root-knot nematode disease of cucumber and a preparation method thereof. Background technique [0002] Root-knot nematode is a highly specialized omnivorous phytopathogenic nematode. It has a wide range of hosts and often damages more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, gourds, lettuce, and cabbage. Spreading some fungal and bacterial diseases is one of the important pests of vegetables in protected areas. With the increase in the production area of ​​vegetables in protected areas, especially since the large-scale promotion of solar greenhouses, the multiple cropping index has increased, coupled with years of repeated cropping, the damage of root-knot nematodes has become increasingly serious, generally resulting in a 10%-20% reduction in production, and up to 30% in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P5/00A01G13/00
Inventor 李敏韩亚楠刘润进
Owner QINGDAO AGRI UNIV
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