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Application of bryophyte as disinfectant for plant explants, disinfectant composition as well as preparation method and use method thereof

A technology of bryophytes and disinfectants, which is applied in the field of plant explant disinfectants, can solve the problems of plant poisoning and unsatisfactory bactericidal effect, and achieve the effects of cheap price, easy popularization and application, and reduced pollution rate

Inactive Publication Date: 2010-01-27
温州医学院附属第二医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] What the present invention aims to solve is the technical problem that the existing disinfectant for plant tissue culture has unsatisfactory bactericidal effect or easily poisons the plant itself. For the first time, bryophytes are used as a disinfectant for plant explant disinfection, and provide The disinfectant composition, which contains bryophyte bactericidal and antibacterial active ingredients, is used for disinfection of plant explants, reduces microbial contamination of plant tissue culture, has the advantages of safety, non-toxicity and good bactericidal performance. The invention also provides Preparation method and use method of this disinfectant composition

Method used

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  • Application of bryophyte as disinfectant for plant explants, disinfectant composition as well as preparation method and use method thereof
  • Application of bryophyte as disinfectant for plant explants, disinfectant composition as well as preparation method and use method thereof

Examples

Experimental program
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Effect test

no. 1 example

[0031] The first embodiment of the present invention is used for the disinfectant composition of plant explant, comprises each component of following weight:

[0032] composition

specific substance

weight

bryophytes

Whip moss

85g

penetrant

Alkylphenol polyoxyethylene ether

2g

stabilizer

Modified castor oil

6g

Co-solvent

Propylene Glycol

0.6g

[0033] Wherein the weight of bryophyte is the weight that bryophyte is made into dry powder (the following examples are the same).

[0034] The preparation process of this plant explant disinfectant composition is:

[0035] 1) Cleaning and grinding: Wash the fresh whip moss with distilled water, dry it in an oven at 40°C, grind and pulverize it into 20 mesh particles;

[0036] 2) Dissolving: 85g of whip moss dry powder was dissolved in 425g of 95% ethanol solution;

[0037] 3) Reverse osmosis and leaching: extract the above solution for 10 min...

no. 2 example

[0046] The second embodiment of the present invention is used for the disinfectant composition of plant explant, comprises each component of following weight:

[0047] composition

specific substance

weight

bryophytes

Feather moss

90g

[0048] penetrant

Laureth Sulfate

3g

stabilizer

Hydroxybenzyl Phosphate

5g

Co-solvent

Acetone

0.5g

[0049] The preparation process of this plant explant disinfectant composition is:

[0050] 1) Cleaning and grinding: Wash the fresh feather moss with distilled water, dry it in an oven at 40°C, grind and pulverize it into 30 mesh particles;

[0051] 2) Dissolving: Dissolve 90g of the dry powder of the feather moss in 900g of 95% ethanol solution;

[0052] 3) Reverse osmosis and extraction: extract the above solution for 12 minutes at 0.07Mpa and 20°C;

[0053] 4) Ultrasonic solution: ultrasonic frequency 20kHz, oscillation time 8 minutes;

[...

no. 3 example

[0061] The third embodiment of the present invention is used for the disinfectant composition of plant explant, comprises each component of following weight:

[0062] composition

specific substance

weight

bryophytes

Mixture of glaucous moss, flat calyx moss and snake moss (1:1:1 by weight)

93g

penetrant

lauroazepam

3g

stabilizer

Alkyl succinate sulfonate

7g

Co-solvent

Cyclohexanone

0.7g

[0063] The preparation process of this plant explant disinfectant composition is:

[0064] 1) Cleaning and grinding: Wash the three kinds of fresh mosses with distilled water, dry them in an oven at 40°C, grind them into 40-mesh particles;

[0065] 2) Dissolving: Dissolve 93g in 1400g95% ethanol solution in total by getting three kinds of moss dry powders in equal amounts;

[0066] 3) Reverse osmosis and extraction: extract the above solution for 20 minutes at 0.05Mpa and 30°C;

[0067] 4) Ultrason...

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Abstract

The invention discloses an application of bryophyte as a disinfectant for plant explants, a disinfectant composition as well as a preparation method and a use method thereof. The composition comprises a bryophyte extract as an active component, a penetrant, a stabilizer and a latent solvent. The invention uses bryophyte to disinfect plant explants for the first time and greatly reduces the pollution rate of tissue culture. The raw materials of the disinfectant composition are from natural plants which are safe, non-toxic and harmless to the environment and plant tissues. The composition has simple preparation method, easy obtainment of raw material, low price and easy popularization and application.

Description

technical field [0001] The invention relates to the disinfection of plant tissue culture technology, in particular to the new application of using bryophytes as a plant explant disinfectant, the disinfectant composition and its preparation method and use method. Background technique [0002] Pollution in plant tissue culture is mainly caused by explant contamination, inoculation operation contamination, and cultivation room contamination; the latter two can be avoided through proficient operation and improvement of the cleanliness of the cultivation chamber, while explant contamination requires selection. Appropriate disinfection method, strict disinfection treatment, and then inoculated on the sterile medium through aseptic operation. According to the traditional surface sterilization treatment technology, after the unused parts of the explants are removed, the following steps need to be processed: (1) The surface sterilization of the explants. Remove some unnecessary part...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01P1/00A61L2/18A61L101/56
Inventor 孙晶胡蓓蕾邹明邬伟孔慧梅刘佳明
Owner 温州医学院附属第二医院
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