Green globular body (GGB) pathway drynaria roosii seedling tissue culture rapid propagation method

Abstract

The invention provides a green globular body (GGB) pathway drynaria roosii seedling tissue culture rapid propagation method. The method is characterized by comprising the following steps of disinfection of explant; induction of GGB; proliferation culture of GGB; differentiation culture of GGB; bud cluster segmentation; and juvenile sporophyte culture. After the drynaria roosii GGB is obtained, inthe proliferation culture of GGB, the use of cytokinin is reduced, and a dark condition is adopted, so that the reduction of the proliferation efficiency of GGB due to differentiation under illumination conditions is obviously improved, the proliferation speed of GGB is increased, and the use cost of light energy is reduced. After the drynaria roosii GGB is differentiated to generate the bud clusters, the root-shaped stem of the tender juvenile sporophyte obtained by segmenting the bud clusters is extremely small, and the time required for culturing the root-shaped stem to reach the volume which can come out of a bottle can be relatively long. By adding the relatively high-concentration of sucrose to treat the juvenile sporophyte in the culture medium, the growth speed of the late root-shaped stem is greatly increased, and then the propagation period of the seedlings is shortened.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining tissue culture seedlings of Quercus fern through the green spheroid approach. Background technique [0002] Green Globular Body (GGB) is a special structure that appears in the fern tissue culture system. It is usually a spherical body composed of many green particles, hence the name. GGB can proliferate continuously in the induction medium, and changing the composition of the medium can make it rapidly differentiate into seedlings, which has the characteristics of high proliferation rate, fast differentiation, and short seedling cycle. Therefore, the use of GGB for the propagation of fern seedlings has great development prospects. At present, GGB tissue culture rapid propagation pathways have been established for a variety of ferns, such as staghorn fern (Ye Xiuxian et al., 2020), pen container tree (Zhang Yan et al., 2020), South...

AI Technical Summary

Problems solved by technology

Some progress has been made in the artificial breeding of Quercus fern. The patent "A Breeding Method of Quercus fern" (Shi Lei and Xu Yan, 2008) reported that sterilized Quercus fern spores were sown on the culture medium to obtain aseptic seedlings. method, which can quickly obtain the sporozoites of Quercus fern, but it is limited by the vigor of the spores. This method still needs to collect the fertile sporophytes of Quercus fern, and properly preserve the collected spores.

Establishing the GGB breeding route of Quercus fern seedlings can break through this limitation, but this method has not been reported so far

In addition, the tissue-cultured seedlings of Quercus fern need to be transplanted out of the bottle when the rhizome is about 0.6-0.8 cm long. Too small rhizome will result in time-consuming operation and low survival rate. So far, there has been no improvement in the promotion of the rhizome of the young sporophyte of Quercus fern in the bottle. fast growing technology