Breeding method for alsophila spinulosa

A technology of Cyaphila spinulosa and Cyaphila spinulosa spores, which is applied in the field of plant propagation, can solve problems such as lack of detailed reports, lagging technology of artificial propagation and cultivation of Cyaphilopinaceae plants, and destruction of wild Cyaphila spinulosa resources, so as to achieve short cultivation period and reduce wild resources and environment Destructive, low-cost effects

Active Publication Date: 2008-06-11
INST OF BOTANY CHINESE ACAD OF SCI
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  • Claims
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Benefits of technology

This patented inventions use spores from plants called Acerola spp., which are tiny pieces that reproduce quickly when they break off or die during their growth process. These creatures have been found naturally throughout North America but nowadays there're many different types of vegetables made by crossing them together like rice seeds (Oryza sativa). They were discovered earlier than any others before 1990 because these rare crops had potential benefits such as nutrients being taken away more efficiently through photosynthesis compared to traditional ways of growing things.

Problems solved by technology

Technological Problem addressed by this patented study describes how cyclopteras belonging to different genus Cyclimbs grow naturally during certain periods when natural vegetables like cranberries occur around the globe. However, these crops may cause harm if left untreated over time without proper protection from predator animals. To address this problem, research into methods for promoting root colonization and reproduction requires studying various parts of the bark structure within specific regions where these Crothers live.

Method used

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  • Breeding method for alsophila spinulosa
  • Breeding method for alsophila spinulosa

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1, alsophila spinulosa (Alsophila spinulosa (Wall.ex Hook.) Tryon) propagation

[0025] 1. Preparation of 1 / 2MS medium

[0026] The composition of 1 / 2 MS medium is the same as MS medium, in which the concentration of macroelements and trace elements is 1 / 2 of MS medium, the concentration of iron salt and organic components is the same as MS medium, agar 7g / L , pH is 5.8. After boiling and dissolving, divide into 100ml Erlenmeyer flasks, and then sterilize by high pressure steam at 121°C for 20min.

[0027] 2. Sterilization of spinosa spores

[0028] Take 5 mg of mature spinulosa (Alsophila spinulosa (Wall.ex Hook.) Tryon) spores and place them in a 1.5ml centrifuge tube, drop them into sterile water, oscillate fully to form a suspension, let stand for 1 hour, and then centrifuge at 4000r / min for 2 minutes. Discard the supernatant; then drop about 1.0ml of a disinfectant solution containing 0.2g / 100ml Tween-80 and 5g / 100ml NaClO into the centrifuge tube to ...

Embodiment 2

[0033]Embodiment 2, alsophila spinulosa (Alsophila spinulosa (Wall.ex Hook.) Tryon) propagation

[0034] 1. Preparation of 1 / 8MS medium

[0035] The composition of 1 / 8 MS medium is the same as MS medium, in which the concentration of macroelements and trace elements is 1 / 8 of MS medium, the concentration of iron salt and organic components is the same as MS medium, agar 6.5g / L , pH 5.5. After boiling and dissolving, divide into 100ml Erlenmeyer flasks, and then sterilize by high pressure steam at 121°C for 20min.

[0036] 2. Sterilization of spinosa spores

[0037] Take 2mg of mature spinosa spores, put them in a 1.5ml centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, let stand for 1h, centrifuge at 4000r / min for 2min, discard the supernatant; then drop about 1.0ml of disinfectant solution containing 0.1g / 100ml Tween-80 and 3g / 100ml NaClO to sterilize the spores, after 4.5min, centrifuge at 4000r / min for 1min, discard the supernatant; fina...

Embodiment 3

[0042] Embodiment 3, the reproduction of Alsophila spinulosa (Wall.ex Hook.) Tryon

[0043] 1. Preparation of full MS medium

[0044] The concentrations of macroelements and trace elements and iron salts are the same as those in MS medium, the concentrations of iron salts and organic components are the same as those in MS medium, the agar is 8g / L, and the pH is 6.0. After boiling and dissolving, divide into 100ml Erlenmeyer flasks, and then sterilize by high pressure steam at 121°C for 20min.

[0045] 2. Sterilization of spinosa spores

[0046] Take 7mg of mature spinosa spores, put them into a 1.5ml centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, let stand for 1h, centrifuge at 4000r / min for 2min, discard the supernatant; then drop about 1.0ml of disinfectant solution containing 0.3g / 100ml Tween-80 and 6g / 100mlNaClO to sterilize the spores, after 6min, centrifuge at 4000r / min for 1min, discard the supernatant; finally rinse with sterile ...

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Abstract

The invention discloses a breeding method for Alsophila spinulosa, in particular to a breeding method for plants (Alsophila spinulosa(Wall.ex Hook.)Tryon). The breeding method for Alsophila spinulosa which is provided by the invention is that spores of Alsophila spinulosa are vaccinated in an MS culture medium and cultivated after disinfection, and are replanted and managed to finally gain spore seedlings. Using small amount of spore material, the invention can quickly breed large numbers of spore bodies which can become seedlings for about 70 days, and the planting survival rate can reach more than 80%. The invention overcomes the shortcomings of the current Alsophila spinulosa breeding technique of long breeding period, high cost and low efficiency, and starts a new path of breeding Alsophila spinulosa by people quickly and enables industrial manufacture of Alsophila spinulosa on a large scale, thereby satisfying demands of Alsophila spinulosa from the markets and simultaneously reducing damage to wild resources and environment. In addition, the invention provides an excellent experiment system for starting researches in the aspects of the genetic breeding of species and for protecting the biology and the like, and the invention has extensive application prospect.

Description

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Claims

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Application Information

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Owner INST OF BOTANY CHINESE ACAD OF SCI
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