Potted ornamental Stenoloma Chusanum Ching spore propagation and maintenance method

A technology for viewing the spores of S. sclerosum and S. spores. It is applied in botany equipment and methods, cultivation, horticulture, etc. It can solve the problems such as the number of spores that have not been raised, and specific effective measures for the follow-up maintenance of spores of S. spores, and achieve low investment. , the effect of simple method

Inactive Publication Date: 2011-08-03
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The predecessors' methods for the spore propagation of S. spp. did not propose an appropriate amo...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Spore reproduction proceeds in the following steps:

[0027] The spores of the black fern fern mature in September to October, and the sporangium cover begins to crack. At this time, the sporophyll leaves are cut off, taken back to the indoor ventilated place to dry, and the spores are shaken off onto white paper and collected for later use.

[0028] Take peat soil, pastoral soil, and river sand, bake at 100-120°C for 8-10 hours, take it out, grind it, pass through a 1mm hole sieve, mix the three kinds of soil according to the volume ratio 2:1:1, and sprinkle some cooled boiling water Wet the soil samples, put them into plastic pots, and use them as culture substrates for the spores of U.

[0029] Sporulation was carried out in mid-September by pouring method, that is, 100 mg of spores collected in that year were weighed, put into a triangular flask with a suction filter interface, 100 ml of cooled boiling water was added, and air was pumped for 1 hour with a vacuum pum...

Embodiment 2

[0035] Collect black fern spores by the method of embodiment 1.

[0036] Prepare the culture medium needed for the spore propagation of Uterus spp. by the method of Example 1.

[0037] According to the method of Example 1 at the beginning of May, the sporulation was carried out by pouring method, and 200 mg of black fern spores collected every other year were weighed, so that the final volume of the spore suspension was 500 ml, and poured to the prepared 1 m 2 substrate surface.

[0038] Place the above pots and pots with the spores of Sarcodon spp. on the window sill with sufficient natural scattered light, and cultivate and manage the prothallus and gametophytes of S. syringae spores until new sporophylls grow.

[0039] According to the method of embodiment 1, carry out seedling training in mid-September, and carry out sub-pot transplanting in late September.

[0040] According to the method of embodiment 1, the sporozoites of the black fern after transplanting are maintai...

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Abstract

The invention discloses a potted ornamental Stenoloma Chusanum Ching spore propagation and maintenance method, which relates to a spore propagation method for ferns. The method is to propagate sporophytes by using spores of Stenoloma Chusanum Ching and maintain the sporophytes. The method comprises: adding 100 to 200 milligrams of spores into 100 to 200 milliliters of cold boiled water, exhausting air for 1 hour, removing floating matters and water fluid, adding 500 to 1,000 milliliters of cold boiled water to prepare spore suspension, spraying each 500 milliliters of suspension onto 1 to 2 square meters of substrate, covering with a thin film after the substrate is completely wetted by the cold boiled water, and culturing under conditions of a temperature of 20 to 28 DEG C, a light intensity of 5,000 to 20,000Lx and a light period of 10 to 12 h/d till light intensity comes out. For maintaining the sporophytes, bamboo leaf humus soil is used as a substrate, the temperature is kept above 10 DEG C, the light intensity is kept between 10,000 to 50,000Lx, the water content in the substrate is 85 to 90 percent, the ventilation is high, and the bamboo leaf humus soil or tea leaf residueis sprayed every 15 to 30 days. The propagated Stenoloma Chusanum Ching sporophytes can be used as an ornamental and medicinal plant resource.

Description

technical field [0001] The invention relates to a method for fern spore propagation and sporophyte maintenance and management, in particular to a method for using Stenoloma chusanum Ching (Stenoloma chusanum Ching) spores to artificially propagate sporophytes and to manage potted sporophytes. Background technique [0002] Sclerophylla is a plant of the genus Pteridophyllaceae, which is mainly distributed in the south of the Yangtze River in my country. Black fern contains flavonoids and phenolic acids, which have the effects of clearing away heat, detoxification, dampness, and hemostasis, and are known as "universal antidote". Black fern is a perennial evergreen herb with lanceolate leaves and multiple splits. The last lobes are wedge-shaped with truncated apex, resembling a lupine. The sporangia are bottle-shaped and born on the top of the lobes, delicate and lovely. The sporophyte of black fern has high ornamental value. [0003] In recent years, the sporozoites of S. sp...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G9/10
Inventor 任冰如李维林梁呈元吴菊兰赵友谊陈剑
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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