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A technique for mutating Trichofern and spores, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effects of simple equipment, shortened reproduction cycle, and low investment
Inactive Publication Date: 2008-11-05
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Abstract
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Problems solved by technology
However, there is very little research on the reproductive variation of D.
Method used
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Examples
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Embodiment 1
[0018] Cut the mature spore leaves, carefully scrape the spores from the edge of the leaves, wrap the collected spores (about 0.1g) with filter paper, soak them in 10mL alcohol with a volume fraction of 75% for 25s on the ultra-clean workbench, and then put Put it into 10mL 1g / L mercury chloride solution for disinfection for 7 minutes, rinse it with sterile water for 5 times, wash the mercury chloride, and finally put it in a sterile petri dish for use.
[0019] Make MS medium, 6g agar and 18g sucrose into 1L of MS spore germination medium with a pH of 5.7-5.8, and divide the prepared medium into 150mL or 200mL culture bottles, and add about 15mL of medium to each bottle , after cooling at room temperature for use.
[0020] Make a suspension of sterile spores with 3 mL of sterile water, use a sterile pipette to take 0.2 mL dropwise into the prepared culture bottle, shake the culture bottle slightly, so that the spores are evenly distributed on the spore germination medium. Th...
Embodiment 2
[0026] Cut the mature spore leaves, carefully scrape the spores from the edge of the leaves, wrap the collected spores (about 0.1g) with filter paper, soak them with 10mL of alcohol with a volume fraction of 75% for 30s on the ultra-clean workbench, and then put Put it into 10mL 1g / L mercuric chloride solution for disinfection for 8 minutes, rinse with sterile water 5 times, wash the mercuric chloride, and finally put it in a sterile Petri dish for use.
[0027] Prepare 1L of 1 / 2MS spore germination medium with pH 5.7-5.8 by making 1 / 2MS, 8g agar and 22g sucrose, divide the prepared medium into 150mL or 200mL culture bottles, and add about 15mL of medium to each bottle , after cooling at room temperature for use.
[0028] Make a suspension of sterile spores with 3 mL of sterile water, use a sterile pipette to take 0.2 mL dropwise into the prepared spore germination culture bottle, shake the culture bottle slightly, so that the spores are evenly distributed on the medium, and c...
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Abstract
The invention relates to a spore reproducing method for Dryopteris varia (L.) Ktunze which can be reproduced fast and is characterized in that the disinfected Dryopteris varia (L.) Ktunze spore is cultured in the culture medium of spore germination and grows into laminar prothallium which is then cultured in multiplication medium for 30 days, in sporophyte inductive medium for 40 days and in sporophyte subculture medium for 20 days in sequence; after forming juvenile sporophyte and grows two to three pieces of juvenile sporophyte leaf, the prothallium is cultured in sporophyte rooting medium for 30 days, is transplanted out of bottle after the base part grows brown fine roots with scales and is transplanted to the soil matrix for culturing after seedling is trained. The spore reproducing method of the invention has little investment and simple equipment, only needs 110 days from the spore germination to the forming of the juvenile sporophyte, obtains large quantities of Dryopteris varia (L.) Ktunze seedlings in short time with the survival rate of the juvenile sporophyte of 90 percent, and leads the Dryopteris varia (L.) Ktunze to become an excellent foliage plant and perform the medical value thereof.
Description
technical field [0001] The invention relates to a spore propagation method of ferns, in particular to a spore propagation method capable of rapidly propagating variant dryopteris varia (L.) Ktunze. Background technique [0002] Variation Phytophthora is a medium-sized herbaceous plant widely distributed in tropical and subtropical regions of my country. It is native to the forest, usually 40-80cm high, with beautiful plant shape, dark green leaves, and evergreen throughout the year. It can not only be developed into a plant with ornamental value It is a foliage plant, and its rhizome has the medicinal effect of clearing away heat and relieving pain, and can be used as medicine, so this kind of fern has good market development value. However, there are very few studies on the reproduction of D. variegata. Contents of the invention [0003] The purpose of the present invention is to develop a method capable of rapidly propagating the mutated Drichopteris. [0004] We obtain...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 王瑞江唐源江董仕勇欧阳婵娟
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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