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Propagation method of cyrtomium fortunei

A technology of stems and spores, applied in the field of stems, can solve the problems of long growth cycle, low efficiency, and unfavorable large-scale production of medicinal stems, etc., so as to reduce the damage to wild resources and the environment, The effect of broad application prospects

Active Publication Date: 2013-01-09
江苏蕨美植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the growth cycle of division propagation is long and the efficiency is low, which is not conducive to the large-scale production of medicinal Guanzhong

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The propagation method of Guanzhong comprises the following process steps:

[0016] Process step 1, culture medium preparation,

[0017] 1) For MS solid medium: 0.7% (W / V) agar, pH 5.8;

[0018] 2) Divide into 100ml Erlenmeyer flasks after boiling, and then sterilize at 121°C and high pressure (0.1Mpa) steam for 20 minutes.

[0019] Process step 2, the sterilization of the spores,

[0020] 1) Wrap the many mature spores with filter paper, tie the paper bag with a thread, put in 4% NaOCl aqueous solution, shake it from time to time, and sterilize for 4 min;

[0021] 2) Rinse 4 times with sterile water;

[0022] 3) Open the paper bag, draw sterile water with a dropper, rinse the spores into a 10 ml centrifuge tube, mix with a vortex mixer to form a suspension, and obtain a sterile spore suspension.

[0023] Process step three, inoculation and cultivation,

[0024] 1) Under sterile conditions, first wet the surface of the above-prepared MS medium with sterile water to...

Embodiment 2

[0035] The propagation method of Guanzhong comprises the following process steps:

[0036] Process step 1, culture medium preparation,

[0037] 1) For MS solid medium: 0.7% (W / V) agar, pH 5.8;

[0038] 2) Divide into 100ml Erlenmeyer flasks after boiling, and then sterilize at 121°C and high pressure (0.1Mpa) steam for 20 minutes.

[0039] Process step 2, the sterilization of the spores,

[0040] 1) Wrap the many mature spores with filter paper, tie the paper bag with a thread, put in 6% NaOCl aqueous solution, shake it from time to time, and sterilize for 4 minutes;

[0041] 2) Rinse 5 times with sterile water;

[0042] 3) Open the paper bag, draw sterile water with a dropper, rinse the spores into a 10 ml centrifuge tube, mix with a vortex mixer to form a suspension, and obtain a sterile spore suspension.

[0043] Process step three, inoculation and cultivation,

[0044] 1) Under sterile conditions, first wet the surface of the above-prepared MS medium with sterile wate...

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Abstract

The invention discloses a propagation method of cyrtomium fortune. The propagation method comprises the following processing steps of: 1, preparing a culture medium; 2, sterilizing cyrtomium fortune spores; 3, inoculating and cultivating; and 4, reculturing seedling and transplanting cyrtomium fortune sporophyte seedling. The propagation method has the following advantages that: deficiencies of long culture period, high cost and low efficiency of the conventional cyrtomium fortune propagation technology are made up, and a new way for artificial propagation of the cyrtomium fortune is developed. Few spore materials are utilized, so that a large amount of sporophytes can be quickly propagated, the seedling is formed in 65 days, and the planting percent is about 97%, so that large-scale industrial production of the cyrtomium fortune becomes possible, thus requirements of the market on the cyrtomium fortune are satisfied, and meanwhile, damage on the wild resources and the environment canbe reduced. Furthermore, the invention further provides a good testing system for developing researches about genetic breeding of species and conservation biology, and wide application prospect is provided.

Description

technical field [0001] The present invention relates to a plant propagation method, in particular to a plant propagation method. Background technique [0002] Cyrtomium fortunei is a perennial evergreen herb belonging to the Dryopteridaceae family. It is used as medicine with rhizomes and petiole residues. It has the functions of clearing away heat, detoxification, hemostasis, and killing insects. It is often used to treat influenza and hepatitis B. , viral pneumonia, functional uterine bleeding and abdominal pain due to worm accumulation; it is also used to treat hypertension, dizziness, headache, chronic lead poisoning and other diseases (Liu Hanzhen, Yu Hao, Xu Wen, He Shengli. Guanzhong's reproduction method and Cultivation techniques. Chinese wild plant resources, 2005, 24 (3): 60-63). At the same time, Guanzhong is a beautiful ornamental plant and a good soil and water conservation plant (Shi Lei. Ornamental ferns. Beijing: China Forestry Press, 2002, 151-152; Liu Han...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张开梅方炎明沈羽万劲
Owner 江苏蕨美植物科技有限公司
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