A kind of enzyme-assisted extraction method of high-purity laver polysaccharide

Abstract

The invention discloses an enzyme-assisted extraction method of high-purity laver polysaccharide, which belongs to the field of biomedicine. The seaweed is dried and ultrafinely pulverized to obtain seaweed powder, and the pulverized seaweed powder is subjected to supercritical CO2 extraction (extraction pressure is 31 MPa, temperature 50 ° C, extraction for 3 hours, separation pressure 5.6 MPa, temperature 55 ° C) degreased, and then The defatted seaweed powder was mixed with 50 times of pure water, 0.5% papain was added to extract at 70°C for 3.5 hours, the filter residue was discarded by centrifugation at 5000 rpm for 15 minutes, the extract was concentrated to 1/4 of the original volume by rotary evaporation, and 80% of Trichloroacetic acid (TCA) was mixed to 2% of the total volume of the solution, and then the solution was reacted in a 70°C water bath for 20 minutes to remove protein, centrifuged at 5000 rpm for 30 minutes to remove the precipitate, and dialyzed in deionized water for 48 hours to remove trichloroacetic acid. The dialysate was collected, and then Slowly add 4 times the volume of 95% ethanol, alcohol precipitation at 4°C for 12h, centrifuge at 5000rpm for 10min, discard the supernatant, wash the precipitate with absolute ethanol, acetone, and ether for three times, respectively, and dry it in an oven at 55°C. Seaweed crude polysaccharide. The yield of papain extraction technology was 26.07%, and the purity reached 84.32%. Compared with hot water extraction, the time was shortened by 50% and the yield was increased by 46%. The obtained laver polysaccharide can be widely used as the base material of medicine and health food.

Description

1. Technical field [0001] The invention relates to a preparation method of laver crude polysaccharide, which belongs to the field of biomedicine, and the product can be widely used in the fields of health products, functional food, biomedicine and the like. 2. Background technology [0002] Seaweed is a seaweed with high nutritional and health value. It has been used as human food and medicine for thousands of years. So far, we have isolated a variety of compounds from laver, which can be divided into polysaccharides, amino acids, proteins, colloids, mannitol, vitamins and other substances. Among them, polysaccharides have anti-tumor growth, anti-virus, calming and tranquilizing, Immunomodulation, heart strengthening, lowering blood fat and blood pressure, protecting liver, lowering blood sugar, anticoagulation, anti-oxidation and anti-aging effects, and has no toxic side effects on normal cells. A large number of research reports show that: laver polysaccharide is the mai...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an enzyme-assisted extraction method of high-purity laver polysaccharides, which overcomes the shortcomings of traditional extraction techniques such as long production cycle, low polysaccharide purity and low extraction yield

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