Extraction process of black corn silk polysaccharide

A corn silk polysaccharide and extraction technology technology, which is applied in the field of extraction technology of natural plants, can solve the problems of black corn silk polysaccharide extraction technology and no report on functional research, etc., and achieves the prevention of cancer cell growth, good color and high recovery rate. Effect

Inactive Publication Date: 2012-06-20
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the extraction process and functional research of black corn silk polysaccharide have not been reported

Method used

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  • Extraction process of black corn silk polysaccharide
  • Extraction process of black corn silk polysaccharide
  • Extraction process of black corn silk polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The calculation method of the purity of black corn silk polysaccharide is: y=0.0113x-0.0417, R 2 =0.9991, where x is the percentage content of the polysaccharide, and y is the light absorption value at 490 nm of the phenol-sulfuric acid reaction of the polysaccharide solution.

[0021] Rough extraction process: Black corn silk raw material 500g, dried at 50°C for 36 hours, crushed, passed through a 60-mesh sieve, added 1.5L of petroleum ether, refluxed at 60-65°C for 1h, cooled to room temperature, evaporated to dry the solvent, added powder residue Extract with distilled water and proteinase K for 1.0 hours (about 40-55°C, pH 6-8), and finally extract with 25-35kHz ultrasonic waves at 55-60°C for 45 minutes, centrifuge, and collect the supernatant; the supernatant is decompressed After concentrating, washing with 95% ethanol, absolute ethanol, acetone and ether successively for several times, and vacuum drying to obtain black corn silk crude polysaccharide. The weight...

Embodiment 2

[0027] Rough extraction process: Black corn silk raw material 500g, dried at 50°C for 36 hours, crushed, passed through a 60-mesh sieve, added 1.5L of petroleum ether, refluxed at 60-65°C for 1h, cooled to room temperature, evaporated to dry the solvent, added powder residue Extract with distilled water and proteinase K for 1.0 hours (about 40-55°C, pH 6-8), and finally extract with 25-35kHz ultrasonic waves at 55-60°C for 45 minutes, centrifuge, and collect the supernatant; the supernatant is decompressed After concentrating, washing with 95% ethanol, absolute ethanol, acetone and ether successively for several times, and vacuum drying to obtain black corn silk crude polysaccharide. The weight ratio of black corn silk to distilled water is 1:20, and the weight ratio of proteinase K to black corn silk raw material is 1.0:200.

[0028] Deproteinization process: with embodiment 1.

[0029] The decolorization process uses 10% hydrogen peroxide for decolorization. The decolorizat...

Embodiment 3

[0033] Rough extraction process: Black corn silk raw material 500g, dried at 50°C for 36 hours, crushed, passed through a 60-mesh sieve, added 1.5L of petroleum ether, refluxed at 60-65°C for 1h, cooled to room temperature, evaporated to dry the solvent, added powder residue Extract with distilled water and proteinase K for 1.0 hours (about 40-55°C, pH 6-8), and finally extract with 25-35kHz ultrasonic waves at 55-60°C for 45 minutes, centrifuge, and collect the supernatant; the supernatant is decompressed After concentrating, washing with 95% ethanol, absolute ethanol, acetone and ether successively for several times, and vacuum drying to obtain black corn silk crude polysaccharide. The weight ratio of black corn silk to distilled water is 1:20, and the weight ratio of proteinase K to black corn silk raw material is 1.5:200.

[0034] Deproteinization process: with embodiment 1.

[0035] The decolorization process uses 90% ethanol decolorization (shaking for 1h, pH is neutral...

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Abstract

The invention discloses an extraction process of black corn silk polysaccharide, which comprises a raw refining process, a protein removing process, a decolorization process and a grading and purifying process, wherein in the raw refining process, protease K water digestion and ultrasound extraction combined method is adopted in the raw refining process, the protein removing process is that sevage method and TDA (trichloroacetic acid) method are alternatively used for deproteinization, an ethanol decolorizing and peroxide decolorizing combined method is adopted in the decolorization process, and DEAE- cellulose (chlorine type) column chromatography method is adopted in the grading and purifying process. Compared with the prior art, the invention has the characteristics of high recovery ratio, excellent decolorization effect, high purity of polysaccharide and the like. The prepared black corn silk polysaccharide has better anticancer and hypolipemic effects.

Description

Technical field: [0001] The invention belongs to the technical field of natural plant extraction technology, in particular to the technical field of black corn silk polysaccharide technology. Background technique: [0002] Corn silk is the dry style and stigma of corn, which is a grass crop. It has the effects of reducing swelling, diuresis, calming the liver and promoting choleresis. It is used to treat hypertension, diabetes, nephritis edema, damp-heat jaundice, gallstones and other diseases. The polysaccharide content in corn silk is as high as 4-5%, which is one of the main active components of corn silk. According to a large number of literature reports, corn silk polysaccharide has many functional properties such as regulating blood sugar, inhibiting tumor growth, regulating body immunity and anti-oxidation. Our country has always had the concept of health preservation when it is black. At present, black corn is very popular in the market. Studies have shown that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P3/06A61P35/00
Inventor 张庭廷孟菁施媚
Owner ANHUI NORMAL UNIV
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