Method for qualitatively and quantitatively analyzing histamine in muscles of miichthys miiuy by surface-enhanced Raman spectroscopy

Abstract

The invention discloses a method for qualitatively and quantitatively analyzing histamine in muscles of miichthys miiuy by surface-enhanced Raman spectroscopy. The method comprises the following steps: mincing the muscles of the miichthys miiuy, making the minced muscles into minced meat, weighing certain parts of equivalent miichthys miiuy muscles to serve as samples, preparing histamine standard solutions of different concentrations, adding the histamine standard solutions into the samples, and using samples at different time points as labeling samples and prediction samples in a storage process; adding the labeling samples and the prediction samples into trichloroacetic acid, and filtering to obtain an extracting solution; measuring the histamine content of the miichthys miiuy meat of the prediction samples through HPLC (High Performance Liquid Chromatography); adding a nanometer enhancer into the extracting solution of the labeling samples, performing Raman spectrum data acquisition, and calculating corresponding Raman characteristic peaks of the histamine by a density functional theory; establishing a quantitative analysis model by using the peak intensity of the Raman characteristic peaks and the histamine content, and measuring the histamine concentration of the muscles of the miichthys miiuy by the quantitative analysis model. By adopting the method, the content of histamine of which the concentration is 1 to 150 mg / L in fresh fish can be detected accurately and quickly.

Description

technical field [0001] The invention relates to the field of histamine detection in fish meat, in particular to a method for qualitatively and quantitatively analyzing histamine in rice fish muscle by surface-enhanced Raman spectroscopy. Background technique [0002] Biogenic amine (Biogenic Amine, BA) is a general term for a class of non-volatile aliphatic, alicyclic or heterocyclic nitrogen-containing organic compounds, which have certain biological activity and widely exist in organisms and various foods. An appropriate amount of biogenic amines has important physiological activities in the body, such as regulating the secretion of neurotransmitters, controlling blood pressure, and participating in immune responses. However, when it is taken in too much or accumulates in the body, it may be toxic to the body. Among them, histamine is the most toxic biogenic amine. Studies have shown that when the histamine content in ingested fish meat is 8-40mg / 100g, it can cause mild ...

AI Technical Summary

Problems solved by technology

[0003] The traditional detection methods for histamine in fish mainly include high performance liquid chromatography (HPLC), fluorescence quantitative method, ion exchange chromatography, capillary electrophoresis and enzyme-linked immunosorbent assay (ELISA), etc. Although these methods have high sensitivity, However, it has certain limitations due to the disadvantages of cumbersome pre-treatment, long detection time, inconvenient instrument carrying and expensive reagents.

Images