34 results about "Bacterial degradation" patented technology

The invention discloses a process for removing nitrite in aquaculture wastewater by immobilized nitrobacteria. The process comprises the preparation of nitrobacteria concentrated solution, the preparation of nitrobacteria immobilized particles, the activation of the nitrobacteria immobilized particles, the preparation of a nitrobacteria immobilized particle reaction packet and the removal of nitrite in the aquaculture wastewater by the reaction packet, wherein the preparation of the nitrobacteria immobilized particles comprises the steps that an aqueous mixture of polyvinyl alcohol, sodium alginate and silicon dioxide is heated and fully dissolved, and is evenly mixed so as to be made into embedding solution; when the embedding solution is cooled down to room temperature, embedding nitrobacteria concentrated solution is added in the embedding solution; after evenly mixed, the mixed solution is dripped into a crosslinking agent; the crosslinking agent consists of calcium chloride solution and saturated boric acid solution which are mixed according to the volume ratio of between 1 to 1 and 1 to 3; and finally, the immobilized particles are filtered out by a piece of gauze. The process has the advantages that nitrite has quick, continuous and stable degradation effect, and maintains high biological activity for a long time; moreover, the reaction packet can be used repeatedly and has the advantages of simple manufacturing method and low cost, thereby having enormous application prospect.

The invention relates to a method for coking wastewater degradation and synchronous power generation by taking coking active bacterium as a biocatalyst. The method is carried out according to the following steps of partitioning a carbon-based active coking microbial film positive electrode and a platinum-loaded carbon fabric negative electrode at two chambers by using a cation membrane or a proton membrane; degrading organic matters and inorganic matters which are high in toxicity and are difficult to be degraded in coking wastewater in an anode anaerobic environment by using the coking active bacterium; allowing oxidation reaction; transferring electrons released through reaction to a positive carbon felt by a medium or a pigment secreted from the bacterium; sequentially transmitting the electrons to a negative electrode through an external wire; and allowing an electron acceptor of the negative electrode to generate reduction reaction. By the process, the treatment of high-toxicity and degradation-resistant coking wastewater is achieved, chemical energy reserved in the process is also converted to electrical energy, a new method is provided for coking wastewater treatment, and meanwhile, a wide prospect is provided for green treatment of the coking wastewater in future.

The invention discloses a bacterium for efficiently degrading residual pesticides carbendazim and application thereof. The bacterium for degrading the residual pesticides carbendazim is stenotrophomonas (Stenotrophomonas sp.) DJL1614 and has the preservation number of CGMCC No.5151 in the China General Microbiological Culture Collection Center (CGMCC). The degradation rate of 10d of the stenotrophomonas (Stenotrophomonas sp.) DJL1614 CGMCC No.5151 provided by the invention on 500ml / L of carbendazim in an inorganic salt culture medium reaches 73.41 percent. Therefore, the strain can efficiently degrade the carbendazim. The stenotrophomonas has wide application prospect on the aspect of repairing the carbendazim pollution of soil.

The invention discloses bacillus coagulans and an application thereof. The bacillus coagulans is preserved in China General Microbiological Culture Collection Center on May 13, 2019, and has the preservation number of CGMCC NO.17801. The invention also discloses a brewing method of table vinegar. The strain is resistant to 8% (v / v) acetic acid, has a wide range of growth temperature (30-55 DEG C),produces acetic acid and highly produces lactic acid, not only can adapt to an acetic acid fermentation harsh environment which is difficult for ordinary microorganisms to grow, but also has low requirements for oxygen, and can grow under both aerobic and anaerobic conditions. Through the use of the strain, the strain can improve the problem of reduction of the content of nonvolatile acid causedby seasonal changes, bacterial degradation and other problems, can significantly improve the content of nonvolatile acid in table vinegar, improves the flavor of the product, improves the quality of the product, and improves the yield of the product to a certain extent.

Water-soluble ethers used as octane number enhancers in gasoline are disturbing components in soils and groundwater even in small amounts due to their strong taste and odour. This invention relates to biological purification of gasoline-contaminated soils and groundwater, more specifically to Variovorax strains being able to degrade ethers and their degradation products and to a mixed bacterial population comprising said bacteria. Further this invention relates to a process for bacterial degradation of ethers and their degradation products and to the use of one or more Variovorax strains of the invention in purifying contaminated soil or water.

The invention provides bacillus aryabhattai GH-9 and an application thereof. The bacillus aryabhattai GH-9 is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No.14519 on August 10th, 2017. The bacillus aryabhattai GH-9 has the following advantages: the bacillus aryabhattai GH-9 can degrade high-ammonia nitrogen and total nitrogen, accumulation of nitrite nitrogen and nitrate nitrogen is avoided, and nitrification and denitrification are performed synchronously. Tests prove that ammonia nitrogen with concentration being 500 mg / L can be degraded by a biological bacterial degradation agent prepared from the bacillus aryabhattai GH-9, the ammonia nitrogen degradation efficiency reaches 77%, and the total nitrogen degradationefficiency can reach 61%.

The invention discloses a method for preparing lambda-carrageenan oligosaccharide by carrying out bacterial degradation on lambda-carrageenan degrading bacteria. The method is characterized by comprising the following steps of: firstly, cultivating the lambda-carrageenan degrading bacteria which can generate extracellular lambda-carrageenan degrading enzymes, subsequently directly adding the bacterium liquid into a reaction liquid which contains 0.5-2.0% by weight of the lambda-carrageenan according to a volume ratio of 1:(0.8-1.2), reacting for 1-5 days at 20-30 DEG C under 100-150rpm, centrifuging so as to obtain supernate-containing oligosaccharide, adsorbing the oligosaccharide by using an activated carbon column, desorbing by using 20-40(v)% ethanol, concentrating, freezing and drying so as to obtain the lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8. The method has the advantages of being high in preparation process, high in yield, high in stability, and applicable to industrial production, and lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8 can be rapidly produced in large scale, so that the method has large application prospects in production of lambda-carrageenan oligosaccharide.

The invention discloses a preparation method for fermentation broth of 1,3-propylene glycol, a treating method for the fermentation broth of 1,3-propylene glycol, application of a bacterial degradation supernatant and a solid containing an organic acid potassium salt and an organic acid salt produced by the treating method to preparation of a water-soluble fertilizer, a preparation method for the water-soluble fertilizer, and the water-soluble fertilizer prepared by using the method, belonging to the field of fermentation of 1,3-propylene glycol. The fermentation broth of 1,3-propylene glycol is obtained through microbial fermentation of glycerin; a neutralizer is used for adjusting the pH value of the fermentation broth in the process of fermentation; and the neutralizer contains more than 50 wt% of potassium hydroxide. The treating method comprises a step of treating the fermentation broth of 1,3-propylene glycol. According to the invention, direct or indirect pollution of by-products to environment is eliminated and production cost for the fertilizer is reduced on the basis that microbial fermentation conditions for production of 1,3-propylene glycol are guaranteed.

The invention belongs to the field of biotechnology and renewable energy sources, and relates to a method for improving the anaerobic energy production efficiency of an algae raw material through bacterial degradation biological pretreatment. The method comprises the following steps: (1), the algae raw material is mixed with a nutritive salt solution to be inoculated to an anaerobic cellulose degrading bacteria seed solution which is cultivated in advance; (2), the seed solution is cultivated under the conditions of thermal insulation and opaque background, the bacterial degradation pretreatment is performed, hydrogen is synchronously produced, hydrolysis of cell walls is promoted, and hydrogen, hydrolysis product ethanol and volatile organic acid are obtained; and (3), after the bacterial degradation synchronous hydrogen production cultivation is finished, anaerobic inoculated sludge containing methanogens is inoculated in fermentation liquor, and accordingly hydrolysis product ethanol, volatile organic acid and algae cell residues after the bacterial degradation suffer anaerobic fermentation for methanogenesis. According to the invention, the algae biomass raw material is subjected to pretreatment through bacterial degradation so as to promote hydrolysis wall breaking of algae cells; and moreover, higher yield of hydrogen is simultaneously obtained in the process of anaerobic fermentation methanogenesis, thereby improving the energy conversion efficiency of the algae biomass raw material.

The invention discloses a method for screening electricity-producing functional bacteria capable of degrading chlorophenols, the screened mixed bacteria and applications thereof. The present invention screens out electricity-producing bacteria capable of degrading chlorophenols from existing marine hydrothermal deposits, uses the bacteria to treat refractory chlorophenol wastewater in a microbial fuel cell system, and generates electricity through the microbial fuel cell system, which can Wastewater resources and energy. The invention has the advantages of low operating cost, convenient operating conditions, etc., and can effectively screen out objective functional bacteria and use them for wastewater treatment. The invention is a treatment process that integrates the screening of electricity-producing bacteria that degrades chlorophenols, sewage treatment, energy, and resource recovery, and has broad application prospects.

The invention discloses chicken fermentation bed padding added with attapulgite clay. The chicken fermentation bed padding added with the attapulgite clay comprises, by mass, 8%-38% of saw dust, 8%-42% of crop stalks, 5%-20% of the attapulgite clay and the balance is rice hulls. According to the chicken fermentation bed padding, only a small amount of higher-priced saw dust is used, and a large number of low-priced rice hulls and crop stalks are used, so the cost of the chicken fermentation bed padding is reduced, composite economic results are improved and resource utilization of the crop stalks is achieved. In addition, the added attapulgite clay can adsorb harmful gas in a henhouse, relieve the pressure of bacterial degradation in the padding and prolong the service life of the padding. After the chicken fermentation bed padding added with the attapulgite clay is eaten by chickens, the toxic and harmful substances in feed and harmful bacteria in intestinal tracts can be adsorbed, the health of the chickens can be improved and chicken production performance and economic benefits are improved.

The invention relates to a screening system for microbial strains with different keratin degradation capabilities. The screening system comprises feather meal plates and transformant secondary-generation bacteria solution. The screening system has the advantages of simple steps, short cycle and high repeatability. Besides, mutants with weakened keratin degradation capabilities or without the keratin degradation capability can be quickly and accurately screened by the screening system, and accordingly foundations can be laid for carrying out deep research on feather degradation mechanisms of bacteria, finding genes related to keratin degradation and constructing new-generation efficient strains.

The invention discloses an agar electrolyte solid salt bridge and a pH sensor using the agar electrolyte solid salt bridge, and in the agar electrolyte solid salt bridge, the electrolyte is prepared from the following components in parts by weight: 8 to 30 parts of KCl, 0.01 to 0.3 part of sodium benzoate, 0.05-0.8 part of Triton X100 and 2 to 5 parts of agar powder. The agar electrolyte solid salt bridge prepared by the invention has a good electrical conductivity, and is resistant to bacterial degradation, capable of withstanding a long-term immersion in hot water with the temperature of 50EDG C or below, resistant to an ultrasonic shock, and has stable bridge differential current performance.

The invention provides bacillus aryabhattai GH-9 and an application thereof. The bacillus aryabhattai GH-9 is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No.14519 on August 10th, 2017. The bacillus aryabhattai GH-9 has the following advantages: the bacillus aryabhattai GH-9 can degrade high-ammonia nitrogen and total nitrogen, accumulation of nitrite nitrogen and nitrate nitrogen is avoided, and nitrification and denitrification are performed synchronously. Tests prove that ammonia nitrogen with concentration being 500 mg / L can be degraded by a biological bacterial degradation agent prepared from the bacillus aryabhattai GH-9, the ammonia nitrogen degradation efficiency reaches 77%, and the total nitrogen degradationefficiency can reach 61%.

Didecylmethylpropyl ammonium iodide and application thereof are provided. Raw materials include 25-30% of water, 10-15% of trimethylamine, 6-15% of hydrochloric acid, 20-25% of didecyl-chain essence,6-8% of epoxy propane, 5-8% of iodine, 15-20% of OP-10, and 2-5% of phosphoric acid. The hydrochloric acid, the didecyl-chain essence and the water are added in order according to a ratio of the formula, and are mixed and fully stirred; then a trimethylamine solution in a formula amount is added; the mixture is cooled; a half of the formula amount of the epoxy propane is added, the temperature rises rapidly, then the rest epoxy propane is added, the temperature rises and a reaction is performed to generate didecylmethylpropyl ammonium salt; cooling is performed; the iodine, the OP-10 and the phosphoric acid are added according to the ratio in the formula; and the mixture is fully mixed and stirred until the reaction solution is uniformly mixed. The didecylmethylpropyl ammonium iodide has strong bacterium killing effects, can degrade toxins generated by bacterial degradation, has obvious sterilization effects for environment and plays a role of water purification.

The invention belongs to the field of biotechnology and renewable energy sources, and relates to a method for improving the anaerobic energy production efficiency of an algae raw material through bacterial degradation biological pretreatment. The method comprises the following steps: (1), the algae raw material is mixed with a nutritive salt solution to be inoculated to an anaerobic cellulose degrading bacteria seed solution which is cultivated in advance; (2), the seed solution is cultivated under the conditions of thermal insulation and opaque background, the bacterial degradation pretreatment is performed, hydrogen is synchronously produced, hydrolysis of cell walls is promoted, and hydrogen, hydrolysis product ethanol and volatile organic acid are obtained; and (3), after the bacterial degradation synchronous hydrogen production cultivation is finished, anaerobic inoculated sludge containing methanogens is inoculated in fermentation liquor, and accordingly hydrolysis product ethanol, volatile organic acid and algae cell residues after the bacterial degradation suffer anaerobic fermentation for methanogenesis. According to the invention, the algae biomass raw material is subjected to pretreatment through bacterial degradation so as to promote hydrolysis wall breaking of algae cells; and moreover, higher yield of hydrogen is simultaneously obtained in the process of anaerobic fermentation methanogenesis, thereby improving the energy conversion efficiency of the algae biomass raw material.

Methodologies, formulations, and kits suitable for decontaminating environments containing bacterial spores by degrading the spores. Formulations contain papain, at least one germinant, and optionally one or more additional enzymes. Methods for killing a bacterial spore include contacting a bacterial spore with the formulation for a duration sufficient to kill the spore, or for a duration sufficient to render the spore susceptible to being killed by a reagent and then contacting the spore with the reagent for a duration sufficient to kill the spore.

A disposable eggshell eco-friendly material and manufacturing method are disclosed. The disposable eggshell eco-friendly material, for volume ratio, includes 50%-80% of calcined eggshell powder, 10%-48% of biodegradable polymer, 1%-5% of natural degradation agent, and 1%-5% of natural binding agent, which are subjected to a mixing and stirring step according to such ratios, and then subjected to a pelletizing step to be first prepared as a plurality of disposable eggshell eco-friendly material pellets, and the disposable eggshell eco-friendly material pellets being then subjected to a shaping and forming step by means of one of film blowing, extruding, vacuum forming, bottle blowing, injecting, and drawing, to obtain a disposable eggshell eco-friendly material product that is disposed of after one time of use, showing properties of being non-toxicant contact for human body and food, water resistance, bacterium resistance, fast degradation, and degradation of one-time-use disposition and burying, soil composted as being fertilizing, to thereby achieving recycled use of waste eggshell and natural degradation after being disposed of after one time use, and being of greening and reusability.

The invention is a composite bioreactor for treating high-concentration refractory wastewater, which consists of three parts: an immobilized biological reaction area, a comprehensive degradation area and a precipitation area. There are engineering bacteria in the immobilized biological reaction area. There are water inlet, air inlet and mud discharge outlet at the bottom, and exhaust holes at the top. The air inlet is connected with the air inlet pipe and the aeration head. There are multiple diversion holes, and the diversion holes are connected to the comprehensive degradation area; there are mixed bacteria in the comprehensive degradation area, the interior is filled with fillers, the bottom is equipped with aeration strips, and the middle part of the outer wall is equipped with multiple liquid diversion grooves. The lower part of the flow tank has a ring-shaped bubble barrier, and the bottom is equipped with multiple sludge return channels. Both the liquid diversion channel and the sludge return channel are connected to the sedimentation area; the upper part of the sedimentation area is provided with a drainage port, and the bottom is provided with a sludge discharge port. The invention effectively treats the waste water through the compound action of the engineering bacteria and the mixed bacteria. The reactor of the invention has compact structure, can greatly reduce the dilution ratio of waste water, has small volume and low cost.

The invention discloses a method for preparing lambda-carrageenan oligosaccharide by carrying out bacterial degradation on lambda-carrageenan degrading bacteria. The method is characterized by comprising the following steps of: firstly, cultivating the lambda-carrageenan degrading bacteria which can generate extracellular lambda-carrageenan degrading enzymes, subsequently directly adding the bacterium liquid into a reaction liquid which contains 0.5-2.0% by weight of the lambda-carrageenan according to a volume ratio of 1:(0.8-1.2), reacting for 1-5 days at 20-30 DEG C under 100-150rpm, centrifuging so as to obtain supernate-containing oligosaccharide, adsorbing the oligosaccharide by using an activated carbon column, desorbing by using 20-40(v)% ethanol, concentrating, freezing and drying so as to obtain the lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8. The method has the advantages of being high in preparation process, high in yield, high in stability, and applicable to industrial production, and lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8 can be rapidly produced in large scale, so that the method has large application prospects in production of lambda-carrageenan oligosaccharide.

A transgenic Arabidopsis thaliana for repairing PCBs-contaminated soil, the invention comprises: subcloning TfdB and BphC genes from the soil metagenome; co-expressing TfdB+BphC plants through the construction of plant expression vectors, and plant genetic transformation mediated by Agrobacterium , screening of transgenic positive plants, transgenic plants repairing PCB 28 contaminated soil and other steps; constructing a pathway similar to bacterial degradation of PCBs in Arabidopsis, improving the tolerance of Arabidopsis to PCBs, reducing the limitation of PCB28 on plant growth, and improving The effectiveness of phytoremediation of PCBs-contaminated soil, and the acquisition of new phytoremediation materials for PCBs-contaminated soil; it has a good application prospect in reducing the threat of pollutants to the ecosystem and improving the ecological safety of PCBs phytoremediation.

The invention provides a phyllostachys pubescens forest fertilization method based on a bacterial degradation small-molecule fish peptide fertilizer. The method comprises the following steps: preparing a bacterial degradation small-molecule fish peptide fertilizer solution by using the bacterial degradation small-molecule fish peptide fertilizer; new bamboos are reserved before fertilization, and the density and age proportion of the standing bamboos are regulated and controlled; a square ditch fertilization method is adopted for fertilization, fertilization is carried out for the first time in late April, a micromolecular fish peptide fertilizer solution is applied firstly, compound fertilizer is applied after 3-5 days, and the fertilization amount ratio of 1-degree bamboos to 2-degree bamboos to 3-degree bamboos is 1: 1: 1; the fertilizing amount ratio of the bacterium solution micromolecule fish peptide fertilizer to the compound fertilizer for preparing the bacterium solution micromolecule fish peptide fertilizer solution is 4: 15; second-time fertilization is conducted in the first ten days of July, the micromolecular fish peptide fertilizer solution is applied firstly, compound fertilizer is applied after 3-5 days, and the fertilization amount ratio of the first-degree bamboos to the second-degree bamboos to the third-degree bamboos is 7: 9: 9; the fertilizing amount ratio of the bacterial degradation small-molecule fish peptide fertilizer to the compound fertilizer for preparing the bacterial degradation small-molecule fish peptide fertilizer solution is 9: 15. The application amount of the compound fertilizer in phyllostachys edulis forest rhizome shoot production can be reduced, the yield and quality of rhizome shoots are improved, and the ecological environment of bamboo forest soil is improved.

The invention provides a protease-producing polylactic acid (PLA) degrading bacterium. The present invention isolates and screens a protease-producing polylactic acid-degrading bacterium Pseudomonas from a soil where polylactic acid / polybutylene terephthalate adipate (PLA / PBAT) mulch film is laid in Weifang, Shandong, and It is named Pseudomonas sp.strain LXM88, and is preserved in China General Microorganism Culture Collection and Management Center with the preservation number (CGMCC 18058). The advantage of the invention is that the bacterium produces protease for degrading PLA, and the bacterium has good pH tolerance and good ability to degrade PLA. The pseudomonas and its protease can be used as enzyme preparations, biological bacteria agents and biological enhancers for environmental restoration of PLA and PLA-based biodegradable materials in the environment, and have good application value.

The invention discloses a PET (Polyethylene Terephthalate) pretreatment method and application thereof in promoting bacterial degradation. The mesorhizobium sp. SCSIO 43976 is preserved in the Guangdong Microbial Culture Collection Center on October 24, 2025, and the preservation number of the mesorhizobium sp. SCSIO 43976 is GDMCC No: 67156. Sorhizobium sp. SCSIO 43976 in bacteria which are derived from a marine ecosystem and have high-efficiency PET degradation capability, a set of high-temperature melting-low-temperature cold quenching pretreatment method which is matched with the Sorhizobium sp. SCSIO 43976 and can effectively reduce the crystallization degree of PET, and a synergistic degradation system formed by the Sorhizobium sp. SCSIO 43976 and the Sorhizobium sp. SCSIO 43976 are used for finally realizing the purpose of degrading the waste PET by using the Sorhizobium sp. SCSIO 43976. Particularly, the high-efficiency biodegradation in the ocean or salt-containing environment is realized. The pretreatment-strain synergistic technology provided by the invention provides an efficient and green solution for pollution treatment of the waste PET in the ocean or high-salt environment, and has important application potential in a biotechnology path of plastic cyclic regeneration.

The present invention relates to a new class of Chloroflexi-like bacteria. The bacteria are thermophilic cellulose degrading bacteria. Compositions and methods for degrading cellulose using the Chloroflexi-like bacteria are also provided.

Disclosed herein is a method for remediating a stored liquid waste by reducing a content of a species capable of being bacterially degraded under anaerobic conditions, the stored liquid waste having bulk properties incompatible with anaerobic biodegradation. The method comprises the following steps (a) and (b), which are repeated until the stored liquid waste has bulk properties compatible with anaerobic biodegradation and contains an amount of the microorganism effective to sustain biodegradation of the species in the stored liquid waste: (a) removing an aliquot of the liquid waste and treating the aliquot by exposing to conditions whereby the species is anaerobically biodegraded by a microorganism capable of biodegrading the species; and (b) returning the treated aliquot back to the stored liquid waste, whereby the bulk properties of the stored liquid waste become more conducive to anaerobic biodegradation.

The invention belongs to the field of environmental microbes, and specifically discloses a Klebsiella sp. bacterium CGMCC13201, which can effectively degrade crude oil in soil contaminated by crude oil, such as oily sludge and oily mud sand. In addition, the invention also discloses a method for using the bacteria to degrade oil stains.