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A method for screening electricity-producing functional bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application

A technology of mixing bacteria and electrical functions, applied in the directions of bacteria, chemical instruments and methods, water pollutants, etc., can solve the problems of water body and aquatic organism pollution, affecting the ecological environment safety of water bodies, and achieve significant economic and environmental significance.

Inactive Publication Date: 2018-03-23
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the untreated chlorophenol-containing wastewater is discharged into the water environment, it will cause serious pollution to the water body and aquatic organisms, affecting the ecological environment safety of the receiving water body

Method used

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  • A method for screening electricity-producing functional bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application
  • A method for screening electricity-producing functional bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application
  • A method for screening electricity-producing functional bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Using marine hydrothermal deposits as the bacterial source, start a single-chamber air cathode microbial fuel cell containing toxic chlorophenols with a concentration of 50mg / L, with a constant external resistance of 510 ohms between the cathode and anode, and monitor the voltage output of the system. When the output voltage is stable After running, the output voltage will be stable within 2-3 cycles, increase the concentration of chlorophenol in the anolyte to 120mg / L, further screen the electricity-producing bacteria that are resistant to high concentrations of chlorophenol and can degrade chlorophenol, and have the ability to degrade chlorophenol Electrobacteria form biofilms on anode graphite felt.

Embodiment 2

[0030] The removal effect of COD in the waste water treatment process of embodiment two

[0031] Chemical oxygen demand (COD), as one of the comprehensive indicators of the degree of water pollution, is an indicator that needs to be monitored in the process of wastewater treatment. Start the microbial fuel cell system, run the system of Example 1 at room temperature and investigate the operation of the system. For the COD degradation of the system, see figure 2 .

[0032] Depend on figure 2 It can be seen that when the initial concentration of TCP is 100mg / L, the COD removal rate reaches 82.34% after 5 days of treatment; when the initial concentration of TCP is 150mg / L, after the same treatment for 5 days, the COD removal rate reaches 83.85%. The COD removal effects of the two were similar, indicating that within the investigation range of the initial TCP concentration, the increase of the initial TCP concentration had little effect on the COD removal effect.

Embodiment 3

[0033] The removal effect of chlorophenol in the waste water treatment process of embodiment three

[0034] For the chlorophenol-containing wastewater treatment system, the first thing to investigate is the removal effect of toxic chlorophenols in the chlorophenol-containing wastewater. This embodiment adopts the method of embodiment one, examines respectively the removal rate of the chlorophenol in different reaction concentration batches, see image 3 . Depend on image 3It can be seen that when the initial TCP concentration increases, the residual TCP concentration in the solution also increases, and the removal rate of TCP decreases. When the initial concentration is 50mg / L, the removal rate of TCP can reach 97.45%. When the initial concentration increased to 150mg / L, the removal rate of TCP decreased to 87.32%.

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Abstract

The invention discloses a method for screening electricity-producing functional bacteria capable of degrading chlorophenols, the screened mixed bacteria and applications thereof. The present invention screens out electricity-producing bacteria capable of degrading chlorophenols from existing marine hydrothermal deposits, uses the bacteria to treat refractory chlorophenol wastewater in a microbial fuel cell system, and generates electricity through the microbial fuel cell system, which can Wastewater resources and energy. The invention has the advantages of low operating cost, convenient operating conditions, etc., and can effectively screen out objective functional bacteria and use them for wastewater treatment. The invention is a treatment process that integrates the screening of electricity-producing bacteria that degrades chlorophenols, sewage treatment, energy, and resource recovery, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to a method for screening electricity-producing functional bacteria capable of degrading chlorophenols, the screened mixed bacteria and applications thereof. Background technique [0002] As important chemical organic raw materials and intermediates, chlorophenols are widely used in industrial production such as wood preservatives, herbicides, fungicides, insecticides, dyes, etc. Chlorophenols are also produced as by-products in waste incineration , paper pulping bleaching and drinking water chlorination disinfection process. Chlorophenols are artificially synthesized exogenous substances with stench and good chemical stability and thermal stability. Natural microorganisms in nature cannot secrete corresponding enzymes to degrade these substances, resulting in the occurrence of such compounds in the environment. long-term residue. As a typical representative of persiste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/36
Inventor 李浩然易爱飞
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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