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Bacteria for efficiently degrading residual pesticide carbendazim and use thereof

A technology of carbendazim and bacterial agent, which is applied in the field of bacteria that can efficiently degrade the residual pesticide carbendazim, and can solve the problems of mammalian toxicity, teratogenicity, and carcinogenicity

Inactive Publication Date: 2011-12-28
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residual period of carbendazim in the soil is relatively long, which can cause human liver disease, lead to chromosomal aberration, and has potential carcinogenic, teratogenic, and mutagenic effects, and is toxic to mammals. Therefore, the degradation of carbendazim is studied becomes more important
[0005] At present, there are relatively few research reports on carbendazim-degrading bacteria at home and abroad

Method used

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  • Bacteria for efficiently degrading residual pesticide carbendazim and use thereof
  • Bacteria for efficiently degrading residual pesticide carbendazim and use thereof
  • Bacteria for efficiently degrading residual pesticide carbendazim and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, Isolation and identification of carbendazim-degrading bacteria DJL1614

[0034] 1. Isolation of carbendazim-degrading bacteria DJL1614

[0035] Add 10 g of soil samples (collected from farmland soil polluted by carbendazim in Beijing, China) to 100 ml of enriched culture solution with a carbendazim concentration of 100 mg / L, culture with shaking at 25°C and 200 r / min for 3 days, and transfer 5 ml of the culture solution to the Inoculated into the enrichment medium with a carbendazim concentration of 200 mg / L, cultured for 3 days, successively enriched, and transferred 5 times to make the carbendazim concentration successively 100, 200, 400, 500, 800 and 1000 mg / L. After the enrichment is completed, take the culture solution and spread it evenly on the same concentration of carbendazim separation medium and inorganic salt medium, and cultivate at a constant temperature of 25°C. After a single colony appears on the plate, pick a larger single colony and transfe...

Embodiment 2

[0056] Example 2. Quantitative determination of the ability of Stenotrophomonas sp. DJL1614CGMCC No.5151 to degrade carbendazim

[0057] 1. Preparation of standard curve for determination of carbendazim

[0058] Prepare a series of standard suspensions with carbendazim standard substance (purchased from Sigma company), according to the feature that carbendazim has a maximum absorption peak at 281nm, measure OD281 by ultraviolet spectrophotometry, and then draw carbendazim assay standard curve, The OD281 determination results of different concentrations of carbendazim are shown in Table 2:

[0059] Table 2 OD281 values ​​of different concentrations of carbendazim

[0060]

[0061] According to the data in Table 1, the equation of the standard curve for the determination of carbendazim is y=0.0769x+0.0064, R 2 = 0.999. Carbendazim assay standard curve as figure 1 shown.

[0062] 2. Quantitative determination of the ability of Stenotrophomonas sp. DJL1614CGMCC No.5151 to ...

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Abstract

The invention discloses a bacterium for efficiently degrading residual pesticides carbendazim and application thereof. The bacterium for degrading the residual pesticides carbendazim is stenotrophomonas (Stenotrophomonas sp.) DJL1614 and has the preservation number of CGMCC No.5151 in the China General Microbiological Culture Collection Center (CGMCC). The degradation rate of 10d of the stenotrophomonas (Stenotrophomonas sp.) DJL1614 CGMCC No.5151 provided by the invention on 500ml / L of carbendazim in an inorganic salt culture medium reaches 73.41 percent. Therefore, the strain can efficiently degrade the carbendazim. The stenotrophomonas has wide application prospect on the aspect of repairing the carbendazim pollution of soil.

Description

technical field [0001] The invention relates to a bacterium capable of efficiently degrading residual pesticide carbendazim and its application. Background technique [0002] The chemical name of carbendazim is N-(2-benzoimidazolyl)-methyl carbamate, and the English chemical name is methyl benzimidazol-2-ylcarbamate. Molecular formula is C 9 h 9 N 3 o 2 , the structural formula is as follows. [0003] [0004] Carbendazim can interfere with the process of nucleic acid synthesis and cell mitosis, and has the effect of preventing and controlling fungal diseases of various crops. It is currently the main fungicide for preventing and controlling plant diseases in agriculture. It has a wide range of applications and mainly acts on rice, peanuts, cotton, and tobacco. , beets, and a variety of vegetables, can prevent melon powdery mildew, tomato early blight, bean anthracnose, blight, rape sclerotinia, green onions, leek gray mold, eggplant, cucumber sclerotinia, melons, be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28
Inventor 孙建光徐晶胡海燕高淼
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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