Preparation method of lambda-carrageenan oligosaccharide

Abstract

The invention discloses a method for preparing lambda-carrageenan oligosaccharide by carrying out bacterial degradation on lambda-carrageenan degrading bacteria. The method is characterized by comprising the following steps of: firstly, cultivating the lambda-carrageenan degrading bacteria which can generate extracellular lambda-carrageenan degrading enzymes, subsequently directly adding the bacterium liquid into a reaction liquid which contains 0.5-2.0% by weight of the lambda-carrageenan according to a volume ratio of 1:(0.8-1.2), reacting for 1-5 days at 20-30 DEG C under 100-150rpm, centrifuging so as to obtain supernate-containing oligosaccharide, adsorbing the oligosaccharide by using an activated carbon column, desorbing by using 20-40(v)% ethanol, concentrating, freezing and drying so as to obtain the lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8. The method has the advantages of being high in preparation process, high in yield, high in stability, and applicable to industrial production, and lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8 can be rapidly produced in large scale, so that the method has large application prospects in production of lambda-carrageenan oligosaccharide.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides, in particular to a method for preparing lambda-carrageenan oligosaccharides with a polymerization degree of 2-8. Background technique [0002] Carrageenan is a natural polyanionic galactose sulfate polysaccharide extracted from red algae, including κ-, ι-, λ- and other types; it is widely used in food, daily chemical, pharmaceutical and other industries; λ - Carrageenan oligosaccharides are low-molecular compounds obtained after degradation of λ-carrageenan. Its structure has many similarities with endogenous heparin (HS), and it belongs to exogenous heparin-like structural compounds. In recent years, many physiological activities of these oligosaccharides and their derivatives have been gradually recognized, and the research on the activities of their degradation products carrageenan oligosaccharides has also been fully carried out, such as anticoagulant, antitumor, antiviral, anti-infl...

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Problems solved by technology

Because this enzyme originates from marine Vibrio, it is expensive, and the stability of the enzyme is poor, the preparation process is loaded down with trivial details, the production cycle is long, the cost is high, and it is not suitable for industrialized production; in the Chinese patent (patent No. CN1513880A), dilute sulfuric acid solution is used as Hydrolyze the medium to produce κ-carrageenan oligosaccharides. The method used in industrial production will cause equipment corrosion, and the discharged waste water will easily lead to environmental pollution, and the neutralization step in the method will produce a large amount of salt, and the acid hydrolysis reaction will cause sulfuric acid The base falls off in different degrees, and the cutting is irregular, which makes the product very complicated, and it is impossible to obtain pure λ-carrageenan oligosaccharides. The target product is not easy to separate, and a desalination process is also required in the production, resulting in complicated processes, increased production costs, and low production efficiency. lower

Therefore, there is also a lack of simple, rapid and industrialized methods for preparing λ-carrageenan oligosaccharides in the current prior art

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