Preparation method of lambda-carrageenan oligosaccharide

A technology of carrageenan oligosaccharides and carrageenan, which is applied in the field of preparation of λ-carrageenan oligosaccharides, can solve the problems of irregular cutting, environmental pollution, complex process, etc., so as to omit the enzyme preparation process, reduce production costs, optimize The effect of the process steps

Active Publication Date: 2013-09-11
广西格新赛致生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because this enzyme originates from marine Vibrio, it is expensive, and the stability of the enzyme is poor, the preparation process is loaded down with trivial details, the production cycle is long, the cost is high, and it is not suitable for industrialized production; in the Chinese patent (patent No. CN1513880A), dilute sulfuric acid solution is used as Hydrolyze the medium to produce κ-carrageenan oligosaccharides. The method used in industrial production will cause equipment corrosion, and the discharged waste water will easily lead to environmental pollution, and the neutralization step in the method will pr

Method used

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  • Preparation method of lambda-carrageenan oligosaccharide
  • Preparation method of lambda-carrageenan oligosaccharide
  • Preparation method of lambda-carrageenan oligosaccharide

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Embodiment 1

[0024] Prepare ATCC medium 1575 solid medium and 120mL liquid medium. The λ-carrageenan-degrading bacterium Pseudoalteromonas carrageenovora was cultured on a slant at 22°C for 1 day, and the slant bacteria were inoculated into 50 mL of liquid medium, and cultured at 130 rpm at 22°C for 2 days, and an equal volume of sterile 1.5% λ-carrageenan (Wako Pure Industry Co., Ltd. Co., Ltd.) in the reaction medium, 30°C, 130rpm reaction for 2d, 10000rpm centrifugation for 30min, the supernatant was collected, 2 times the volume of washed activated carbon was installed in a 2.6×30cm chromatographic column, and the sugar solution was directly chromatographically The column was adsorbed for 5 hours, and impurities such as unadsorbed oligosaccharides and salts were eluted with pure water, and then eluted with 30% ethanol to obtain an eluate containing λ-carrageenan oligosaccharides with a low degree of polymerization. Concentrate in vacuo and freeze-dry to obtain a white powder λ-carragee...

Embodiment 2

[0026] Prepare ATCC medium 1575 solid medium and liquid medium, and sterilize. The λ-carrageenan-degrading bacteria Pseudoalteromonas carrageenovora was cultured on a slant at 22°C for 1 day, inoculated into 20mL liquid medium, cultured at 22°C and 130rpm for 1d, and expanded step by step until it was placed in a 30L fermenter, and the medium in the tank was ATCC medium 1575 liquid Medium, the inoculum amount is 2%, culture at 22°C, 130rpm for 2 days, add an equal volume of reaction medium containing 2.0% λ-carrageenan, react at 30°C, 130rpm for 4 days, plate filter, collect the filtrate, wash 2 times the volume The activated carbon is installed in a 1×2m chromatographic column, the sugar solution is directly adsorbed on the chromatographic column for 5 hours, the unadsorbed oligosaccharides and salts and other impurities are eluted with pure water, and then eluted with 40% ethanol to obtain The eluate containing λ-carrageenan oligosaccharides with a low degree of polymerizati...

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Abstract

The invention discloses a method for preparing lambda-carrageenan oligosaccharide by carrying out bacterial degradation on lambda-carrageenan degrading bacteria. The method is characterized by comprising the following steps of: firstly, cultivating the lambda-carrageenan degrading bacteria which can generate extracellular lambda-carrageenan degrading enzymes, subsequently directly adding the bacterium liquid into a reaction liquid which contains 0.5-2.0% by weight of the lambda-carrageenan according to a volume ratio of 1:(0.8-1.2), reacting for 1-5 days at 20-30 DEG C under 100-150rpm, centrifuging so as to obtain supernate-containing oligosaccharide, adsorbing the oligosaccharide by using an activated carbon column, desorbing by using 20-40(v)% ethanol, concentrating, freezing and drying so as to obtain the lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8. The method has the advantages of being high in preparation process, high in yield, high in stability, and applicable to industrial production, and lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8 can be rapidly produced in large scale, so that the method has large application prospects in production of lambda-carrageenan oligosaccharide.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides, in particular to a method for preparing lambda-carrageenan oligosaccharides with a polymerization degree of 2-8. Background technique [0002] Carrageenan is a natural polyanionic galactose sulfate polysaccharide extracted from red algae, including κ-, ι-, λ- and other types; it is widely used in food, daily chemical, pharmaceutical and other industries; λ - Carrageenan oligosaccharides are low-molecular compounds obtained after degradation of λ-carrageenan. Its structure has many similarities with endogenous heparin (HS), and it belongs to exogenous heparin-like structural compounds. In recent years, many physiological activities of these oligosaccharides and their derivatives have been gradually recognized, and the research on the activities of their degradation products carrageenan oligosaccharides has also been fully carried out, such as anticoagulant, antitumor, antiviral, anti-infl...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12R1/01
Inventor 上官巧灵陈海敏严小军
Owner 广西格新赛致生物科技有限公司
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