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Bacillus aryabhattai GH-9 and application thereof
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A technology of Bacillus agri pollution, etc.
Active Publication Date: 2018-01-19
BEIJING UNIV OF AGRI +1
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However, currently, the screened high-efficiency ammonia nitrogen degrading bacteria can only degrade low concentrations of ammonia nitrogen, and the degradation of high concentrations of ammonia nitrogen is not ideal.
For example, CN105255754A discloses a bacterium capable of degrading ammonia nitrogen and total nitrogen. The bacterial name is Pseudomonassp.ZXY-1. Sewage stench and odorpollution caused by ammoniacal nitrogen and total nitrogen
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Embodiment 1
[0030] Example 1: Isolation, purification and identification of Bacillus arborii GH-9
[0031] Take 10ml of the landfill leachate from Nangong Garbage Composting Field in Beijing, inoculate it into a 250ml Erlenmeyer flask containing 100ml of sterilized enrichment medium, shake it well, and culture it with shaking at a temperature of 28°C and a rotational speed of 160r / min. Enriched for 7 days, supplemented every other day (NH 4 ) 2 SO 4 , to eliminate the inability to utilize NH 4 + -N microorganisms; then take 1ml of the bacterial solution in the enrichment medium and spread it on the separation medium plate, culture it upside down in a 28°C incubator, and pick the single colonies grown on the plate for repeated isolation and purification to obtain a A single strain was named GH-9.
[0032] The bacterial strain GH-9 has the following morphological characteristics: Gramstaining reaction is positive, short rod-shaped, capable of producing spores, such as figure 1 ; Milk...
Embodiment 2
[0034] Embodiment 2: the preparation of biodegradant
[0035] (1) Inoculate Bacillus arborii GH-9 into the screening medium for fermentation culture, control the temperature at 32°C, pH 7.0, rotation speed 200r / min, and fermentation time 48 hours to obtain the mother liquor of biological bacteria degradation agent;
[0036] (2) Then, the mother liquid of the biological bacteria degradation agent is added to the fermentation medium according to the ratio of 1% for fermentation and cultivation, the temperature is controlled at 32°C, the pH is 7.0, the rotation speed is 150r / min, and the fermentation time is 48 hours, the biological bacteria degradation agent is obtained .
Embodiment 3
[0037] Embodiment 3: the application of bacillus arborii GH-9
[0038] The samples retrieved from the big tower of Beijing Nangong Biological Deodorization Tower were tested for ammonia nitrogen index: the ammonia nitrogen content of the big tower was 514mg / L, the total nitrogen content was 627mg / L, the pH value was 5.42, and the ammonia gas content at the gas outlet was 2.31mg / m3 3 .
[0039] Add the biological bacteria degradation agent prepared in Example 2 into the sewage of Nangong Tower according to the inoculum amount of 1%, and cultivate it under 32°C aeration environment for 4 days. The efficiency reaches 61%, the pH value is 6.53, and the ammonia gas content at the gas outlet is 0.79mg / m 3 , The ammonia degradation rate at the gas outlet reaches 65.8%.
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technical field [0001] The invention relates to a strain of bacteria and its application, in particular to a strain of bacillus arborii GH-9 and its application. Background technique [0002] Nitrogen in water mainly includes organic nitrogen and inorganic nitrogen, organic nitrogen includes protein, amino acid and organic amine, etc., inorganic nitrogen includes ammonia nitrogen, nitrite nitrogen and nitrate nitrogen, which are collectively called total nitrogen. The form of nitrogen is different, and its harmfulness in water is also different. For example, during the decomposition of protein, a large amount of ammonia nitrogen will be produced, and ammonia nitrogen will cause harm to the water body, pollute the water body, and generate odor. Therefore, the total nitrogen content in water is one of the important indicators to measure water quality, and the determination of total nitrogen is helpful to evaluate the pollution status of water body and the degree of self-purifi...
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