Acinetobacter junii for producing bio-surfactant and application of acinetobacter junii

A technology of Acinetobacter jelly, biological surface, applied in microorganism-based methods, microorganisms, microorganisms, etc., can solve the problem of no separation, etc., and achieve the effect of excellent emulsifying activity and ability to degrade saturated alkanes

Active Publication Date: 2017-03-22
CHINA UNIV OF PETROLEUM (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although Acinetobacter genus Acinetobacter has been reported to be ubiquitous in oil reservoir systems, so far there have been no reports of Acinetobacter junii isolated from oil reservoir systems

Method used

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  • Acinetobacter junii for producing bio-surfactant and application of acinetobacter junii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the separation, identification and preservation of the product surfactant bacterial classification of the present invention

[0033] 1. Isolation of bacteria producing biosurfactant

[0034] Among the present invention, the screening method of producing biosurfactant microorganisms is: take 10mL of the water sample collected from Xinjiang Oilfield and insert it into 100mL of sterilized crude oil inorganic salt culture medium (2% crude oil, v:v), 35 ℃ , 150rpm constant temperature shaking culture for 72h, select the experimental group with a high degree of emulsification and dispersion of crude oil, take 100μL of fermentation broth and spread it on the LB agar plate medium, and cultivate it at 35°C for 48h; pick single colonies of different shapes, and draw on the LB agar plate medium Line purification, cultured at 35°C for 48h. Pick a single colony and inoculate it into crude oil inorganic salt medium (g / L: NaNO 3 8. MgSO 4 0.5, KH 2 PO 4 1,K 2 HPO...

Embodiment 2

[0050] Embodiment 2, the degradation experiment of BD to crude oil

[0051] Seed bacterium liquid: Inoculate BD bacteria in the enrichment medium (prepared with reference to the prior art, the basic composition includes: Yeast 5g / L, NaCl 10g / L, Peptone 10g / L. Cultivate to a concentration of 10 8 -10 9 cells / mL, as the seed solution, for the experimental research of this example.

[0052] Crude oil inorganic salt medium (g / L): NaNO 3 8. MgSO 4 0.5, KH 2 PO 4 1,K 2 HPO 4 1. FeSO 4 ·7H 2 O0.02, Na 2 MoO 4 0.02, CaCl 2 2H 2 O 0.06, crude oil 0.5% (W / V), pH 7.2.

[0053] GC-MS analysis of crude oil degradation by BD bacteria:

[0054] Use LB medium to activate the strains, and then prepare crude oil inorganic salt medium (g / L): NaNO 3 8. MgSO 4 0.5, KH 2 PO 4 1,K 2 HPO 4 1. FeSO 4 ·7H 2 O 0.02, Na 2 MoO 4 0.02, CaCl 2 2H 2 O 0.06, Xinjiang crude oil 0.5% (W / V), pH 7.2, medium is subpackaged in the 250mL Erlenmeyer flask, every bottle 100mL, inocu...

Embodiment 3

[0059] Embodiment 3, product extraction and analysis of producing surfactant microorganism BD

[0060] Activation and fermentation of strains

[0061] (1) Strain activation: 100 μl of BD strain preserved in a glycerol tube was inoculated into LB medium, shaken and cultured in a constant temperature shaker at 37° C. for 8 hours, as a seed solution.

[0062] (2) Initial fermentation conditions: the seed solution is inserted into 200mL fermentation medium (the components of the fermentation medium are: sunflower oil 20g / L, NaNO 3 10g / L, KH 2 PO 4 0.8g / L, Na 2 HPO 4 0.8g / L, yeast 0.5g / L, pH 7.2), in a constant temperature shaker at 37°C, shake culture at 150r / min for 96h to obtain the initial fermentation broth.

[0063] Experiment 1. Purification of fermentation products

[0064] In the fermentation medium (the components of the fermentation medium are: sunflower oil 20g / L, NaNO 3 10g / L, KH 2 PO 4 0.8g / L, Na 2 HPO 4 0.8g / L, yeast 0.5g / L, pH 7.2) into 5% of the in...

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Abstract

The invention provides acinetobacter junii for producing a bio-surfactant and application of the acinetobacter junii. The preservation number of the acinetobacter junii is CGMCC No. 13206. The acinetobacter junii has good surface activity producing performance, can produce multiple types of rhamnolipid surfactants through metabolism, can be applied to microbial enhanced oil recovery, has the characteristics of being high in oil reservoir environment adaptability, good in biological stability and the like, and can effectively increasing the enhance oil recovery.

Description

technical field [0001] The invention belongs to the field of application of bioengineering technology in microbial oil recovery breeding, and specifically relates to a kind of Acinetobacter jonesii isolated from oil reservoirs to produce biosurfactant and its application, including the use of the strain to ferment and produce surface active agent, and the application of the strain in enhanced oil recovery. Background technique [0002] In oil extraction technology, water flooding is an effective way of enhancing oil recovery widely used in the world. However, after waterflooding, a large amount of oil remains in the formation. chemical flooding, CO 2 Foam flooding, steam flooding and other oil recovery techniques are used to enhance oil recovery. However, these extraction techniques require higher economic and energy costs, as well as damage to the environment. Microbial oil recovery is a low-cost, environmentally friendly tertiary oil recovery technology, which has attr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/44C09K8/582C09K8/584C12R1/01
CPCC09K8/582C09K8/584C12N1/20C12P19/44C12N1/205C12R2001/01
Inventor 孙珊珊张忠智董浩董汉平侯吉瑞刘同敬罗一菁张志勇崔凯刘楚晗
Owner CHINA UNIV OF PETROLEUM (BEIJING)
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