Bacillus subtilis and application thereof

A technology of Bacillus subtilis and bacteria agent, which is applied in the direction of bacteria, petroleum industry, microorganisms, etc., and can solve the problem of less bacteria

Active Publication Date: 2006-10-11
DAQING OILFIELD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many bacteria have been isolated from oil reservoirs, but there are fewer bacteria that can be used in microbial oil recovery

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and preservation of Bacillus subtilis L-510 CGMCC No.1563

[0031] Enrichment medium (g / L): liquid paraffin 20g, K 2 HPO 4 10g, NaH 2 PO 4 5g, (NH 4 ) 2 SO 4 2g, yeast powder 1g, MgSO 4 ·7H 2 O 200mg, CaCl 2 2H 2 O 1mg, FeSO 4 ·7H 2 O 1mg, the rest is water, pH7.0-7.2. Plate medium (g / L): peptone 10g, beef extract 3g, NaCl 5g, agar 18g, the rest is water, pH7.0.

[0032] Inoculate the oily sewage sample collected from Pubei Oilfield into the enrichment medium, and carry out enrichment culture at 45°C and 150rpm. After 3-7 days of cultivation, take the sample with obvious emulsification and dispersion effect of crude oil, collect the fermentation liquid, and repeat The above operations domesticate and stabilize the performance of wild strains, while the samples with insignificant emulsification and dispersion effects are eliminated in turn; then the samples with better emulsification effects are separated and screened on the plate med...

Embodiment 2

[0036] Embodiment 2, indoor characteristic analysis of Bacillus subtilis (Bacillus subtilis) L-510 CGMCC No.1563

[0037] 1. Detection of the influence of Bacillus subtilis L-510 CGMCC No.1563 on the physical properties of crude oil

[0038] Nutrient solution formula (g / L): K 2 HPO 4 10g, NaH 2 PO 4 5g, (NH 4 ) 2 SO 4 2g, yeast powder 0.5g, beef extract 0.5g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.1g, FeSO 4 ·7H 2 O 0.1g, the rest is water, pH7.0-7.2.

[0039] 1. Detection of the influence of Bacillus subtilis L-510 on the composition of crude oil

[0040] Detect the influence of Bacillus subtilis L-510 on the composition of crude oil group with following method:

[0041] 1) Collect oil samples from oil wells whose well numbers are 13-2702, 13-P29 and 12-2655, and then use column chromatography to detect the family composition of the oil samples with reference to the analysis method in SY / T5119-1995;

[0042] 2) Take a 250mL triangular flask, add 5-10g of oil s...

Embodiment 3

[0100] Embodiment 3, use Bacillus subtilis (Bacillus subtilis) L-510 CGMCC No.1563 to carry out single well throughput field test and test bacteria

[0101] 1. Single well huff and puff field test

[0102] Test oilfield: Daqing Pubei oilfield, the crude oil of this oilfield has the characteristics of low specific gravity, low saturation pressure, high wax content (25%), low gel content, large ground-saturation pressure difference, and large solubility coefficient; the formation water is sodium bicarbonate type, the chloride ion content is 2674-2902mg / L, and the total salinity is 8490-9789mg / L.

[0103] Test bacteria: Bacillus subtilis L-510 CGMCC No.1563 (hereinafter referred to as Bacillus subtilis L-510)

[0104] Use Bacillus subtilis (Bacillus subtilis) L-510CGMCC No.1563 of the present invention to carry out single well huff and puff field test, the test method is: first select 10 test wells in this oil field, and 2 wells are selected, and the selection standard is: produ...

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Abstract

This invention discloses a Bacillus subtilis strain L-510 CGMCC No.1563 and the application thereof. The lab analysis shows that the changes of the crude oil processed by this strain have the following characteristics: (1)the rang of the light components is broadened, the content of the non-hydrocarbon components is averagely increased by 2.09%, and the content of asphaltene is averagely decreased by 1.29%, (2) the rate of wax is averagely decreased by 17.56%, and the rate of colloid is averagely decreased by 26.54%, (3)the flow capacity of the crude oil is improved, and the initial apparent viscosity number of the viscosity-temperature curve is decreased by 50% campared to the value before the procession. The result of the microbial huff and puff field test using this strain conducted in Pubei Oilfield shows that, in the four months after being processed by this strain, the effective wells are upto 80%, the liquid output is increased by 44.8%, and the daily oil production is increased by 47.4%. This strain can be widely used in reservoir engineering field, especially in the Microbial Enhanced Oil Recovery(MEOR) field.

Description

technical field [0001] The invention relates to the field of microbial oil recovery, in particular to a bacillus subtilis and its application in oil recovery engineering. Background technique [0002] Petroleum is a non-renewable energy source. In order to effectively utilize petroleum resources, countries all over the world are looking for efficient methods that can enhance the recovery rate, so as to exploit the crude oil that remains in the formation and is difficult to recover by conventional oil recovery methods. Since the 1980s, many countries have carried out research on the technology of enhancing oil recovery by using microorganisms. After more than 20 years of hard work, this technology has made great progress. [0003] Microbial Enhanced Oil Recovery technology can be called Microbial Enhanced Oil Recovery (MEOR, Microbial Enhanced Oil Recovery) technology, which refers to the use of useful metabolites of microorganisms or the performance of microorganisms that ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/125C12S1/00
Inventor 郭万奎石成方乐建君崔宝文杨振宇石梅韩德金刘春林朱凯韩培慧伍晓林庞彦明王凤兰赵国忠李莉
Owner DAQING OILFIELD CO LTD
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