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Low-permeability reservoir microbial enhanced oil recovery composite preparation and application method thereof

A technology for microbial oil recovery and compound preparation, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex and unsatisfactory microbial oil recovery construction operations, etc., and achieves low production cost, good application prospects, and good prospects. The effect of environmental adaptability

Active Publication Date: 2015-02-25
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to cooperate with fracturing operations, which is relatively complicated, and cannot meet the construction operations of microbial oil recovery under certain conditions.

Method used

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  • Low-permeability reservoir microbial enhanced oil recovery composite preparation and application method thereof
  • Low-permeability reservoir microbial enhanced oil recovery composite preparation and application method thereof
  • Low-permeability reservoir microbial enhanced oil recovery composite preparation and application method thereof

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Effect test

Embodiment 1

[0029] This embodiment provides a low-permeability oil reservoir microbial oil recovery composite preparation, which is mixed by fermentation cultures of Pseudomonas aeruginosa CGMCC1.10452 and two kinds of Bacillus subtilis CGMCC1.2172 and CGMCC1.2166, according to the volume ratio 4:3:3 mixed preparation, and the total number of microbial colonies in the composite preparation is greater than 9×1011CFU / ml.

[0030] The preparation method of the above-mentioned low-permeability oil reservoir microbial oil recovery composite preparation includes the following steps: first activate Pseudomonas aeruginosa CGMCC1.10452, Bacillus subtilis CGMCC1.2172, and Bacillus subtilis CGMCC1.2166 in LB solid medium; The fermentation liquid medium corresponding to the bacterial strain was inoculated and cultured for 48 hours, and then the three bacterial solutions were combined to prepare a microbial oil recovery composite preparation.

[0031] The formula of LB solid medium is: tryptone 10g / L,...

Embodiment 2

[0049] Deoiling Effect of Microbial Oil Recovery Compound Preparation on Crude Oil

[0050]Triassic Chang 6 oil reservoir Wangyao block crude oil and quartz sand were mixed at a volume ratio of 1:1, and aged at 37°C for 1 week. Take 20g of dried quartz sand with oil, add 50ml of 3% microbial oil recovery compound preparation, blank control Wangyao block oilfield formation water, and simulate formation temperature at 35°C for constant temperature cultivation for 48h. After 0.5, 1, 3, 6, 12, 24, and 48 hours, take out and measure the crude oil floating on the liquid surface, and test the deoiling efficiency of the microbial oil recovery composite preparation. The experimental results are shown in Table 1. With the microbial oil recovery With the prolongation of the action time of the compound preparation, the oil layer on the liquid surface in the beaker where the microbial oil recovery compound preparation is added gradually becomes thicker, and the bottom quartz sand gradually...

Embodiment 3

[0054] Experiment of removing plugging and restoring rock core permeability with microbial oil recovery compound preparation

[0055] Using 3% microbial oil recovery compound preparation to carry out the plugging removal experiment, record the change of permeability before and after, and evaluate the plugging removal recovery efficiency of the microbial oil recovery compound preparation. Take the artificial core, connect the normal phase with brine, and measure the permeability k0 of the brine; connect the normal phase with crude oil mixed with sand to form a blockage; flow the reverse phase with brine, measure the permeability k1 of the brine before removing the plugging; Soak for 24 hours to remove the blockage; connect the brine in the normal phase, measure the permeability k2 of the brine after the blockage is removed, and see Table 2 for the experimental results. The results show that the recovery rate of 3% microbial oil recovery composite preparation can reach 97.6% and...

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Abstract

The invention belongs to the technical field of microbial enhanced oil recovery and particularly relates to a low-permeability reservoir microbial enhanced oil recovery composite preparation and an application method thereof. The low-permeability reservoir microbial enhanced oil recovery composite preparation is mixed and prepared by pseudomonas aeruginosa CGMCC1.10452 and the fermentation culture of bacillus subtilis CGMCC1.2172 and CGMCC1.2166. During use of the composite preparation, the injection stage is divided into four slugs which are completed discontinuously, and finally even oil layer displacement and crude oil elution are achieved. The microbial composite preparation has the advantages that oil field water injection efficiency can be effectively improved so as to increase crude oil recovery efficiency, the preparation is non-hazardous to environments and human bodies, promising the application prospect in the field of low-permeability reservoir microbial enhanced oil recovery, simple in process, low in production cost and good in environmental adaptability to low-permeability reservoirs, and crude oil recovery efficiency can be increased evidently.

Description

technical field [0001] The invention belongs to the technical field of microbial oil recovery, and in particular relates to a low-permeability oil reservoir microbial oil recovery compound preparation and its application method. Background technique [0002] Petroleum is an important energy source in industrial society and plays a pivotal role in economic and social development. However, the crude oil reserves in the whole world are decreasing day by day, and many oil fields in production have entered the middle and late stages of exploitation, and the proportion of depleted mines and heavy oil mines is gradually increasing, which increases the difficulty of mining. This brings a broad research space for us to enhance oil recovery through various technical means. [0003] Microbial Enhanced Recovery Technology (MEOR) is a new technology that has been researched and applied more and more maturely in recent years. The best cheap oil recovery method for oily and depleted rese...

Claims

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Application Information

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IPC IPC(8): E21B43/20C09K8/582C12N1/20C12R1/385C12R1/125
CPCC12N1/20E21B43/16
Inventor 申坤李斌张昊白玉军张蓓董昭易永根周晓红李国营钟智勇
Owner PETROCHINA CO LTD
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