Method for monitoring advantageous flora in microbial enhanced oil recovery

Abstract

The invention relates to a method for extracting the total DNA of a microorganism sample during the microbial oil recovery process, which comprises the steps of selecting a proper PCR-DGGE primer for extracting the total DNA of a microorganism sample by adopting the method for monitoring an advantageous flora in microbial enhanced oil recovery, wherein the sequence of the PCR-DGGE primer is DQF338: 5'-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGACTCCTACGGGAGGCAGCAG-3' DQR534:5'-ATTACCGCGGCTGCTGG-3'. By adopting the above primer, the actual amount of microorganisms in an oil deposit can be accurately reflected. Meanwhile, the distribution, migration and change of the oil recovery microorganisms in the oil deposit can be accurately and quickly analyzed. Thus, the method provides an important theatrical instruction for improving oil recovery ratio.

Description

Technical field: [0001] The invention relates to a method for monitoring dominant bacterial groups in the microbial oil recovery process in the field of oil recovery. Background technique: [0002] With the development of tertiary oil recovery technology, Daqing Oilfield has entered the late stage of high water cut development. For low permeability oil fields, microbial oil recovery technology has a good application prospect. It is of great significance to maintain high and stable production of oil fields and sustainable development. [0003] The analysis and cognition of microbial communities in oil reservoirs is the basis for the development and application of microbial oil recovery technologies. Traditionally, the method of pure culture is used to study and understand the microbial community in oil reservoirs. Due to the existence of traditional culture methods: (1) The environmental conditions are not fully considered and simulated, resulting in only a very small number...

AI Technical Summary

Problems solved by technology

Traditionally, the method of pure culture is used to study and understand the microbial community in oil reservoirs. Due to the existence of traditional culture methods: (1) The environmental conditions are not fully considered and simulated, resulting in only a very small number of microorganisms (0.01% ~ 1%) being able to grow in (2) Artificial culture conditions have different selectivity and enrichment effects on microorganisms, so the relative situation of the detected microorganisms in terms of type, quantity and function is determined by selection and enrichment. (3) It is impossible to accurately study and understand the laws of the microbial ecosystem in the oil reservoir; (4) It is impossible to guide people to scientifically regulate the microbial community in the oil reservoir to improve oil recovery. Yield purpose and other issues

Therefore, there is a large discrepancy between the cognition of the oil reservoir microbial community and the actual situation using the traditional culture method, which leads to the inability to accurately and effectively regulate the oil reservoir microbial community, and cannot effectively improve oil recovery.

[0004] At present, the microorganisms isolated by pure culture technology only account for about 0.1% to 15% of the natural world. With the development of molecular biology and phylogenetic analysis methods, especially the PCR amplification and probe hybridization technology based on 16SrRNA gene, it can It allows us to more accurately and comprehensively understand the diversity of microbial populations in specific ecosystems. There are still relatively few reports on the study of microbial ecosystems in oil reservoirs by means of molecular biology.

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