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A technology of microbial oil recovery and oil displacement, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., which can solve the problems of no research rules and methods that can be followed
Active Publication Date: 2009-07-29
DAQING OILFIELD CO LTD +1
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[0008] Analyzing research data at home and abroad, it is found that microbial oil recovery technology has been developed for more than half a century since 1926. However, the use of microbial/chemical flooding to
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Embodiment 1
[0031] Embodiment 1, screening, cultivation and preservation of bacterial strains
[0032] Enrichment medium: K 2 HPO 4 0.1-0.5%, KH 2 PO 4 0.1-0.5%, NH 4 NO 3 0.1-0.2%, MgSO 4 ·7H 2 O0.01-0.1%, FeSO 4 0.001-0.01%, CaCl 2 0.001-0.01%, ZnSO 4 0.01-0.02%, CuSO 4 , 0.0005-0.001%, urea 0.01-0.1%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 14 million) 0.05-0.1%, the rest is water, pH 6.8-7.5, 121 ℃, Sterilize for 15-20min. Cultivate on a shaker at 45°C and 120rpm for 5-7 days.
[0033] Oil plate medium: K 2 HPO 4 0.1-0.5%, KH 2 PO 4 0.1-0.5%, NH 4 NO 3 0.1-0.2%, MgSO 4 ·7H 2 O0.01-0.1%, FeSO 4 0.001-0.01%, CaCl 2 0.001-0.01%, ZnSO 4 0.01-0.02%, CuSO 4 , 0.0005-0.001%, urea 0.01-0.1%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 14 million) 0.05-0.1%, agar 1.5-2%, the rest is water, pH6. 8-7.5, 121°C, sterilize for 15-20min.
[0053] Embodiment 2, artificial cemented rock core flooding experiment
[0054] Experimental model: The experimental models are all artificial heterogeneous cemented cores, the coefficient of variation Vk=0.72, the model length cm=31.131.3, and the cross-sectional area cm 2 =12.38-12.46.
[0055] Experimental water, oil, bacteria liquid:
[0056] The saturated water used in the experiment is brine artificially prepared according to the average formation water of Daqing Oilfield (the formula is: NaCl 0.3977% CaCl 2 0.0028%, MgCl 2 6H2O 0.0046%, Na 2 SO 4 0.0093%, NaHCO 3 0.2634%), the mineralization degree is 6778mg / L.
[0057] The oil used in the experiment is the crude oil taken from Well-J5--P25 in North 2 Area of Daqing Oil Production No. 3 Plant. The viscosity of the crude oil after dehydration is 47.2mPa·s.
[0058] The injection water is the injection water of the polymer injection station of Daqing Oil Production No. 3 Plant.
[0059] Bacterial liquid used i...
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Abstract
The invention discloses a microbial oil production method, including a process of displacement of reservoir oil by using bacterial liquid after polymer flooding of oil layers; the bacterial liquid is a mixed liquid or zymotic fluid of bacillus fusiformis 6# CGMCC No.2439, Potsdam Bacillus brevis Po CGMCC No.2441 or Bacillus licheniformis U1-3 CGMCC No.2437 singly mixed with a culture medium, or compatible liquid of the three with the culture medium. The experimental results of model displacement of reservoir oil show that the bacterial liquid is used for displacement of reservoir oil after the polymer flooding, so that recovery ratio is increased by 3-5 percent (OOIP), the bacterial liquid, coupled with polymer protection slug flooding, is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio is increased by about 7-9 percent (OOIP), and a method with the bacterial liquid displacement and chemical displacement combined is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio can be increased by 13% (OOIP). The experimental results show that the method of the invention can be widely applied to the field of oil production engineering, especially the field of microbial intensified oil production, and is suitable for large-scale popularization and application.
Description
technical field [0001] The invention relates to the field of microbial oil recovery, in particular to a method for recovering residual oil after polymer flooding by using microorganisms. Background technique [0002] Petroleum is a non-renewable energy source. In order to effectively utilize petroleum resources, countries all over the world are looking for efficient methods to enhance the recovery rate, so as to exploit the crude oil that stays in the formation and is difficult to recover by conventional oil recovery methods. Since the 1980s, many countries have carried out research on the technology of enhancing oil recovery by using microorganisms. After more than 20 years of hard work, this technology has made great progress. [0003] Polymer flooding in Daqing Oilfield has made breakthroughs through key scientific and technological breakthroughs in the eighth and ninth five-year plans of the country. It has entered the stage of industrial application from pilot field tes...
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