Tool box and kit for rapid extraction of nucleic acid, and method for rapid extraction of nucleic acid

Abstract

The invention provides a tool box and a kit for rapid extraction of nucleic acid, and a method for rapid extraction of nucleic acid, which are mainly used for rapidly extracting nucleic acid (including RNA and DNA) from various clinical samples. According to the invention, a nucleic acid extraction device and a reagent are combined as an integrative device which mainly comprises an injection syringe, a hollow adaptor, a nucleic acid adsorbed film tube, a sample adding needle, various pipes for accommodating different reagents, a pipe frame and other parts; a pure nucleic acid solution for downstream experiments can be obtained through the following steps of lysis treatment on a clinical sample, suction by the injection syringe, adsorption of nucleic acid by a nucleic acid adsorbed film, rinse of the nucleic acid adsorbed film with a rinse liquid, elution of nucleic acid with an eluant, and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a toolbox, a kit for rapidly extracting nucleic acids from various clinical samples and a method for rapidly extracting nucleic acids from clinical samples. Background technique [0002] (1) Nucleic acid extraction [0003] Nucleic acid extraction has become the most important and basic operation in molecular biology experimental techniques, but there are currently many nucleic acid extraction schemes, and they are complicated and simple. The completion of molecular diagnosis, nucleic acid sequencing, functional genomics and other related experiments all depend on the successful extraction of nucleic acid. The traditional operation process can be fully automated, but these expensive instruments cannot be widely used. In developing countries, the vast primary medical units cannot be equipped with complex instruments, so it is necessary to provide an economical nucleic acid extraction...

AI Technical Summary

Problems solved by technology

The completion of molecular diagnosis, nucleic acid sequencing, functional genomics and other related experiments all depends on the successful extraction of nucleic acid. The traditional operation process can be fully automated, but these expensive instruments cannot be widely used. my country is still developing In China, the vast grassroots medical units cannot be equipped with complex instruments. Therefore, it is necessary to provide an economical nucleic acid extraction device

[0024] CN101684463A proposes a device for quickly extracting nucleic acid from micro-clinical samples, which consists of three parts: a pointed suction pipe, a filter with membrane and a suction device (syringe or plastic bubble), which completes the nucleic acid adsorption, cleaning and eluting process through three suctions , can quickly and easily extract nucleic acid from the sample, but the fly in the ointment is that the combination of the three parts of the pointed suction pipe, the filter with membrane and the suction device is single and fixed installation, when the suction device is made of plastic bubbles (CN101684463A figure 2 , image 3 ) the third suction for nucleic acid elution cannot actually be completed (the waste liquid from the first two suctions cannot be discharged, and the eluent with nucleic acid is mixed in the waste liquid), and when using a syringe as a suction device, the eluent with nucleic acid Dehydration into the syringe will not only contaminate the syringe to form biological waste, but also cause the nucleic acid in the trace sample to be contaminated and lost to the syringe, resulting in inaccurate detection

[0025] On the other hand, due to time-limited requirements in clinical testing and diagnosis, rapid extraction and testing of nucleic acids is required, and the classification of reagents and testing equipment in conventional laboratories is too complicated to perform targeted testing, making it difficult to achieve efficient testing; and grassroots units often Due to limited conditions, it is difficult to get together testing reagents and equipment in a short time, so rapid nucleic acid extraction technology is not easy to popularize

[0026] In addition, there are a variety of nucleic acid extraction kit products in clinical practice, but only reagents are provided, and additional suitable tools need to be found to complete the detection operation, which sometimes affects the detection due to incomplete or unsuitable equipment.

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