Method for preparing cubilose extract with strong antioxidation
A technology for extracting liquid and bird's nest is applied in the field of preparing cosmetics and/or health care products, and can solve problems such as affecting the properties of final cosmetics or health care products, and increasing the cost of high-concentration enzymes.
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Embodiment 1
[0054] Embodiment 1. The hydrolysis effect of the trypsin of different concentration
[0055] According to the above-mentioned general method, use the edible protease provided by Hong Kong Maritime Co., Ltd. with an enzyme activity of 50,000 U / g, and the weight ratio of edible trypsin to bird's nest water-soluble protein is 1:1000, 1:750, 1:500 , 1:200 and 1:1 edible protease hydrolyzed Indonesian white swallow strips to prepare bird's nest extract.
[0056] The protein molecular weight distribution before and after enzymatic hydrolysis was determined by conventional SDS-polyacrylamide gel electrophoresis. The results are shown in figure 1 middle.
[0057] The protein content was measured by the Coomassie brilliant blue method, and the protein extraction rate was calculated according to the following formula:
[0058] Protein extraction rate (%)=(measured protein content in bird's nest extract / total amount of bird's nest water-soluble protein)*100. The results are shown in...
Embodiment 2
[0065] Embodiment 2: the hydrolysis effect of different bird's nest raw materials
[0066] Using the same method as in Example 1, the edible protease was measured to prepare bird's nest extract by hydrolyzing Indonesian white bird's nest, Indonesian white bird's nest and Thai bird's nest with a weight ratio of 1:1000 edible trypsin and bird's nest water-soluble protein . The bird's nests were all purchased from a professional bird's nest supplier under the Hong Kong Federation of Chinese Medicine Merchants. The results are shown in Figure 5 middle.
[0067] Table 2 lists the antioxidant capacity of different batches of bird's nest extracts after enzymatic hydrolysis.
[0068] Table 2: Antioxidant capacity of different batches of bird's nest extracts after enzymatic hydrolysis
[0069] Different batches of bird's nest extract
Embodiment 3
[0070] Embodiment 3: the hydrolysis effect of different proteases
[0071] Using the same method as in Example 1, different proteases were used to hydrolyze Indonesian white bird's nest strips with a weight ratio of 1:1000 of protease to bird's nest water-soluble protein to prepare bird's nest extract. The results are shown in Image 6 middle. The edible trypsin used was the same as that in Example 1. The trypsin and alkaline protease used in the laboratory were both purchased from SIGMA-ALDRICH, ST LOUIS, and both had an enzyme activity of 50,000 U / g.
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