Method for preparing cubilose extract with strong antioxidation

A technology for extracting liquid and bird's nest is applied in the field of preparing cosmetics and/or health care products, and can solve problems such as affecting the properties of final cosmetics or health care products, and increasing the cost of high-concentration enzymes.

Active Publication Date: 2013-12-11
SHENZHEN MANNAY COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The use of high concentrations of enzymes increases the cost
In addition, when the final extract is further used as a raw material for cosmetics or h...

Method used

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  • Method for preparing cubilose extract with strong antioxidation
  • Method for preparing cubilose extract with strong antioxidation
  • Method for preparing cubilose extract with strong antioxidation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1. The hydrolysis effect of the trypsin of different concentration

[0055] According to the above-mentioned general method, use the edible protease provided by Hong Kong Maritime Co., Ltd. with an enzyme activity of 50,000 U / g, and the weight ratio of edible trypsin to bird's nest water-soluble protein is 1:1000, 1:750, 1:500 , 1:200 and 1:1 edible protease hydrolyzed Indonesian white swallow strips to prepare bird's nest extract.

[0056] The protein molecular weight distribution before and after enzymatic hydrolysis was determined by conventional SDS-polyacrylamide gel electrophoresis. The results are shown in figure 1 middle.

[0057] The protein content was measured by the Coomassie brilliant blue method, and the protein extraction rate was calculated according to the following formula:

[0058] Protein extraction rate (%)=(measured protein content in bird's nest extract / total amount of bird's nest water-soluble protein)*100. The results are shown in...

Embodiment 2

[0065] Embodiment 2: the hydrolysis effect of different bird's nest raw materials

[0066] Using the same method as in Example 1, the edible protease was measured to prepare bird's nest extract by hydrolyzing Indonesian white bird's nest, Indonesian white bird's nest and Thai bird's nest with a weight ratio of 1:1000 edible trypsin and bird's nest water-soluble protein . The bird's nests were all purchased from a professional bird's nest supplier under the Hong Kong Federation of Chinese Medicine Merchants. The results are shown in Figure 5 middle.

[0067] Table 2 lists the antioxidant capacity of different batches of bird's nest extracts after enzymatic hydrolysis.

[0068] Table 2: Antioxidant capacity of different batches of bird's nest extracts after enzymatic hydrolysis

[0069] Different batches of bird's nest extract

Embodiment 3

[0070] Embodiment 3: the hydrolysis effect of different proteases

[0071] Using the same method as in Example 1, different proteases were used to hydrolyze Indonesian white bird's nest strips with a weight ratio of 1:1000 of protease to bird's nest water-soluble protein to prepare bird's nest extract. The results are shown in Image 6 middle. The edible trypsin used was the same as that in Example 1. The trypsin and alkaline protease used in the laboratory were both purchased from SIGMA-ALDRICH, ST LOUIS, and both had an enzyme activity of 50,000 U / g.

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Abstract

The invention relates to a method for preparing a cubilose extract, which comprises the step of adding protease to a cubilose raw material to hydrolyze water-soluble cubilose protein, wherein the addition amount of the protease is 4500-40,000U per 100g water-soluble cubilose protein. The invention further relates to an application of the cubilose extract obtained by the method in preparing cosmetics or health care products.

Description

technical field [0001] The present invention generally relates to a method for preparing a bird's nest extract with a strong antioxidant effect, and the use of the obtained bird's nest extract in preparing cosmetics and / or health care products. Background technique [0002] The bird's nest is a nest built by the saliva secreted by various birds of the swift family (Apodidae) and the genus Collocalia or mixed with their down feathers and condensed on the cliffs. The main production areas are Indonesia, Malaysia, Vietnam, etc. According to the "Supplements to Materia Medica": "Bird's nest is sweet and flat, nourishes lung yin, reduces phlegm and relieves cough, nourishes and clears. Those who cannot clear up the descendants can be cured with this." Chinese medicine believes that bird's nest has the effect of nourishing yin and moistening dryness, nourishing qi and nourishing the middle. It is used to treat diseases such as deficiency, coughing and asthma, hemoptysis and chron...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K8/64A61Q19/02A61Q19/08A61K38/01A61P39/06A23L1/29A23L33/00
Inventor 陈嘉伦詹华强温志昌张艳芳温川蓉
Owner SHENZHEN MANNAY COSMETIC
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