Use of natural active substances in cosmetic or therapeutic compositions

a technology of natural active substances and compositions, which is applied in the preparation of peptides, hydrolysed protein ingredients, plant ingredients, etc., can solve the problems of sex, sex, and long process cycle, and achieve the effect of reducing the number of steps and ensuring the effect of sex

Inactive Publication Date: 2011-03-03
LESAFFRE & CIE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0293]Tests are carried out on normal human epidermal keratinocytes NHEK seeded in the wells of a 96-well plate in a KSFM medium (without serum). The lipid synthesis, the FLG (filaggrin), CK10 (cytokeratin) and TGK (transglutaminase K) synthesis and the hyaluronic acid synthesis, are assessed in the presence of different concentrations of hydrolysed yeast proteins (from 0.04 mg/ml to 1 mg/ml). Three culture wells are made under condition.
[0294]Calcium is used as positive control for lipid synthesis and FLG, CK10, TGK synthesis and retinoic acid as positive control for hyaluronic acid synthesis.
[0295

Problems solved by technology

The implementation of such a production process shows many drawbacks, in particular, the durati

Method used

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  • Use of natural active substances in cosmetic or therapeutic compositions
  • Use of natural active substances in cosmetic or therapeutic compositions
  • Use of natural active substances in cosmetic or therapeutic compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

Obtaining of Hydrolysed Yeast Proteins According to this Invention Equipment and Methods

[0264]An aqueous suspension of yeast cells of Saccharomyces cerevisiae, having a content of dry matter within 12 and 30% by mass, is subjected to a thermal treatment from 1 to 3 hours within 70° C. and 90° C. (in order to deactivate the endogenous cell enzymes). This thermal treatment induces a yeast plasmolysis that allows separating thereafter the insoluble fraction from the soluble fraction, being the soluble fraction limited. The separation of the solubilised fraction from the insoluble fraction is carried out through several successive stages of centrifugation and washing with water (at least 2 successive stages, preferably at least 3).

[0265]The insoluble fraction recovered, having a content of dry matter within 12 and 25% by mass, is then hydrolysed by adding at least one exogenous protease during at least 18 hours at a temperature of 45° C. to 65° C. For example, the protease is the papain...

example 2

Effect of the Hydrolysed Yeast Proteins According to the Invention on the Expression Profile of Keratinocytes and Fibroblasts

Equipment and Methods

[0285]The effect of the hydrolysed yeast proteins according to the invention on the expression profile of normal human epidermal keratinocytes and normal human dermal fibroblasts is valued on DNA microarrays.

[0286]The first microarray has 164 genes of human keratinocytes, especially involved in cell growth, differentiation, adhesion, communication, and death.

[0287]The second microarray has 143 genes of human fibroblasts, especially involved in cell growth, adhesion, communication, synthesis and extracellular matrix degradation and stress.

[0288]Normal human epidermal keratinocytes and normal human dermal fibroblasts are cultured for 24 h or 96 h in the presence or absence of hydrolysed yeast proteins of example 1. Cells are then washed and their RNA is extracted and purified. cDNA is obtained from this RNA through reverse transcription. The...

example 3

Moisturising, Anti-Aging and Firmness Properties of Hydrolysed Yeast Proteins According to the Invention

Equipment and Methods

[0292]The hydrolysed yeast proteins used, are those described in example 1.

(i) Moisturising Effect

[0293]Tests are carried out on normal human epidermal keratinocytes NHEK seeded in the wells of a 96-well plate in a KSFM medium (without serum). The lipid synthesis, the FLG (filaggrin), CK10 (cytokeratin) and TGK (transglutaminase K) synthesis and the hyaluronic acid synthesis, are assessed in the presence of different concentrations of hydrolysed yeast proteins (from 0.04 mg / ml to 1 mg / ml). Three culture wells are made under condition.

[0294]Calcium is used as positive control for lipid synthesis and FLG, CK10, TGK synthesis and retinoic acid as positive control for hyaluronic acid synthesis.

[0295]Negative control is constituted by the sole culture medium.

[0296]Lipid synthesis is analysed through Phosphoimaging and the hyaluronic acid synthesis is assessed throu...

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Abstract

The invention relates to cosmetic or therapeutic compositions that contain hydrolysed yeast proteins as an active ingredient, to the use of said cosmetic or therapeutic compositions, and to a method for cosmetic treatment.

Description

FIELD OF THE INVENTION[0001]The purpose of this invention is the use of natural active substances in cosmetic and in therapeutic compositions, these substances being hydrolysed yeast proteins obtained from the insoluble yeast fraction.TECHNICAL BACKGROUND[0002]For several years, protein hydrolysates have given rise to an interest in cosmetic and therapeutic applications.[0003]Protein hydrolysates can have different origins: animal, in particular fish, vegetable, or fungal, for example yeast.[0004]The presence or absence of biological activity of a protein hydrolysate depends, in particular, on the nature of the start proteins.[0005]Thus, the hydrolysis of fish proteins allowed obtaining hydrolysed proteins with a particular spatial structure recognised by certain receptors. Activities of hormonal and opioid type have thus been emphasised (Legal and Stenberg, Biofutur, No. 179, 1998, pages 61 to 63).[0006]Certain documents of the prior art mention the use of yeast protein hydrolysate...

Claims

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Application Information

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IPC IPC(8): A61K8/64C07K14/39A61K38/16A61P17/00A61Q19/00A61Q19/08A61Q17/04
CPCA61K8/0212A61K8/99A61Q5/00A61Q5/006A61Q5/12A61Q19/007A61Q19/00A61Q19/008A61Q19/08A61K8/64A61K38/01A61Q7/00A61K8/9728A61P17/00A61P17/06A61P17/08A61P17/10A61P17/16A61K8/02
Inventor JUSTEN, PETERBORREILL, DOMINIQUE MARIE NOELLEMARQUES, WILLIAM
Owner LESAFFRE & CIE
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