Skin repair composition and skin care product

Abstract

The present invention belongs to the technical field of skin care, and discloses a skin repair composition and a skin care product, wherein the skin repair composition comprises the following components by weight percentage: 30-50% wheat germ oil, 1-5% ceramide 3, 1-10% sodium hyaluronate, and 40-60% bifid yeast fermentation product lysate. The present invention utilizes bifid yeast fermentation product lysate to help restore the activity of fibroblasts and keratinocytes, repair cell damage and protect the components in the extracellular matrix ECM, and further strengthens the damage repair speed of ECM by compounding sodium hyaluronate and ceramide, helps cells and extracellular matrix maintain a healthy state and quickly repair damage, and helps strengthen intercellular lipids and cell adhesion at the same time, and finally helps form a good skin surface sebum film through the synergistic effect of wheat germ oil and sodium hyaluronate, and the four cooperate with each other to significantly improve the skin repair effect.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to a skin repair composition and a skin care product. Background Art

[0002] As time goes by, the skin will undergo a natural aging process. This aging process causes the skin to become dry and thin, and the skin's lipid synthesis ability is damaged, which further leads to increased transepidermal water loss, reduced skin barrier function, and fragile skin. Some people with congenitally thin dermis and weak barrier function also need skin repair. In addition, the environment is constantly affecting and micro-damaging the skin, including photodamage caused by ultraviolet radiation, which will decompose collagen in the dermis and other components of the extracellular matrix (ECM), affecting the activity and function of fibroblasts, further reducing the possibility of fibroblasts repairing ECM proteins and promoting the synthesis of healthy collagen. Skin damage caused by electronic radiation, mechanical damage and bad living habits, etc., all require skin repair.

[0003] Prior art 1: Chinese patent application CN112972276A discloses a moisturizing and hydrating facial cream and a preparation method thereof. The moisturizing and hydrating facial cream comprises the following ingredients by weight percentage: deionized water 78-82%, wheat germ oil 5-8%, avocado tree fat 1-2%, sodium hyaluronate 3-4%, ceramide 1-2%, trehalose 1-2%, squalane 1-2%, cactus extract 0.5-1%, aloe vera leaf juice 0.3-1%, emulsifier 2-3%, thickener 0.5-1%, and preservative 0.1-0.15%.

[0004] The moisturizing and hydrating facial cream provided by the above technical solution has the characteristics of significant hydrating and water-locking effects. After external use, it can effectively replenish skin moisture, deeply nourish the skin, promote metabolism, and improve skin quality.

[0005] Similarly, prior art 2: Chinese patent application CN108042432A discloses a moisturizing eye cream and a preparation method thereof. The moisturizing eye cream has the following formula in weight percentage: jojoba oil 1-8%, sea buckthorn fruit oil 0.1-0.8%, rosehip oil 1-8%, wheat germ oil 1-8%, glycerol 1-8%, butylene glycol 1-10%, sorbitol 1-6%, sodium hyaluronate 0.1-0.8%, ceramide 0.1-0.8%, elastin 0.1-0.8%, cholesterol 0.1-0.8%, carbomer 9400.1-0.9%, EG 1.1-8%, NaOH 0.01-1%, Gemini emulsifier 0.1-0.8%, monoolein 0.1-0.8%, plant essential oil 0.5-1.5%, PEG-400 5-1.5%, phenoxyethanol 0.1-0.8%, hexylene glycol 0.1-0.8%, cinnamone 0.1-0.8%, tocopherol 0.1-0.8%, disodium EDTA 0.1-0.8%, dehydroacetic acid 0.01-0.08%, and the balance is water.

[0006] The above technical solution is rich in plant essential oil ingredients, which helps users get closer to nature, fully replenishes skin moisture, moisturizes the skin, makes the skin plump and moisturized, and is green and healthy, without fragrance or artificial coloring.

[0007] Both of the above technical solutions contain two substances, sodium hyaluronate and ceramide. The purpose of both technical solutions is to improve the skin's moisturizing ability. However, sodium hyaluronate and ceramide actually have certain skin repair effects in addition to moisturizing effects. Ceramide 3 exists in the intercellular lipids of the stratum corneum and combines with cell surface proteins through ester bonds to play an intercellular connection role. When the content of ceramide in the epidermis decreases with age or other factors, the adhesion of keratinized cells in the stratum corneum decreases, resulting in a loose structure of the stratum corneum and a decrease in skin barrier function. Supplementation of ceramide 3 can help repair damaged intercellular lipids, strengthen the stratum corneum barrier, and rebuild cell adhesion. Sodium hyaluronate, as the main structural component of ECM, can help maintain the healthy environment of ECM faster, and through interaction with receptors, it can also help repair damage in ECM faster.

[0008] Therefore, a skin repair composition based on sodium hyaluronate and ceramide can be developed to improve the skin repair function. Summary of the invention

[0009] One of the purposes of the present invention is to provide a skin repair composition to solve the problem that sodium hyaluronate and ceramide are mostly used as moisturizing compositions in the prior art, and no in-depth research on compound combinations in the direction of skin repair has been conducted. The skin repair composition has relatively excellent skin repair effect.

[0010] Another object of the present invention is to provide a skin care product, which uses a skin repair composition obtained by compounding wheat germ oil, ceramide 3, sodium hyaluronate and bifida yeast fermentation product lysate as a skin repair ingredient and has excellent skin repair effect.

[0011] To achieve the above object, the present invention provides a skin repair composition, which comprises the following components by weight percentage: 30-50% wheat germ oil, 1-5% ceramide 3, 1-10% sodium hyaluronate, and 40-60% bifida yeast fermentation product lysate.

[0012] Preferably, the composition comprises the following components by weight percentage: 30% wheat germ oil, 5% ceramide 3, 5% sodium hyaluronate, and 60% bifida yeast fermentation product lysate.

[0013] Preferably, the composition comprises the following components by weight percentage: 39% wheat germ oil, 1% ceramide 3, 10% sodium hyaluronate, and 50% bifida yeast fermentation product lysate.

[0014] Preferably, the composition comprises the following components by weight percentage: 50% wheat germ oil, 1% ceramide 3, 9% sodium hyaluronate, and 40% bifida yeast fermentation product lysate.

[0015] The invention also discloses a skin care product, comprising the skin repairing composition, wherein the content of the skin repairing composition is ≥1%.

[0016] Furthermore, the content of the skin repair composition in the skin care product is 1-3%.

[0017] As a further preferred embodiment, the content of the skin repair composition in the skin care product can be 1%, 1.5%, 2%, 2.5%, or 3%.

[0018] Beneficial Effects

[0019] Compared with the prior art, the present invention has at least the following advantages:

[0020] (1) The present invention discloses a skin repair composition, which utilizes a lysate of a bifid yeast fermentation product to help restore the activity of fibroblasts and keratinocytes, repair cell damage and protect components in the extracellular matrix (ECM). By compounding sodium hyaluronate and ceramide, the speed of ECM damage repair is further enhanced, helping cells and the extracellular matrix to maintain a healthy state and quickly repair damage, while helping to strengthen intercellular lipids and cell adhesion. Finally, through the synergistic effect of wheat germ oil and sodium hyaluronate, a good skin surface sebum film is formed. The four cooperate with each other to significantly improve the skin repair effect.

[0021] (2) The present invention prepares a skin composition by compounding wheat germ oil, ceramide 3, sodium hyaluronate and bifida yeast fermentation product lysate within a certain dosage range, thereby maximizing the skin's repair function and damage recovery function;

[0022] (3) The present invention conducts in-depth research on the compounding of sodium hyaluronate and ceramide, and uses a large amount of bifid yeast fermentation product lysate to synergize with sodium hyaluronate and ceramide to improve the speed of skin damage repair, and uses wheat germ oil to establish a protective barrier of oil, thereby providing a better environment for skin repair and significantly improving the skin repair effect;

[0023] (4) Further studies have found that the recommended usage amount of the skin repair composition of the present invention in skin care products is no more than 3.0%. If the usage amount is less than 1%, the product efficacy is not obvious, and if the usage amount is more than 3.0%, the product efficacy is not significantly improved. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0025] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0026] In the following examples and comparative examples, bifid yeast fermentation product lysate was purchased from Guangzhou Miaosen Biotechnology Co., Ltd., wheat germ oil was purchased from Guangzhou Minghui Fine Chemical Co., Ltd., ceramide 1 and ceramide 3 were purchased from Guangzhou Zhenbang Daily Chemical Co., Ltd., sodium hyaluronate was purchased from Huaxi Biotechnology Co., Ltd., olive oil was purchased from Guangzhou Weisheng Olive Oil Co., Ltd., sodium PCA was purchased from BASF (China) Co., Ltd., and lactic acid bacteria fermentation lysate was purchased from CLR, Germany.

[0027] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0028] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0029] Example 1

[0030] A skin repairing composition is obtained by uniformly mixing 30 parts of wheat germ oil, 5 parts of ceramide 3, 5 parts of sodium hyaluronate and 60 parts of bifid yeast fermentation product lysate.

[0031] Example 2

[0032] A skin repairing composition is obtained by uniformly mixing 39 parts of wheat germ oil, 1 part of ceramide 3, 10 parts of sodium hyaluronate and 50 parts of bifid yeast fermentation product lysate.

[0033] Example 3

[0034] A skin repair composition is obtained by uniformly mixing 50 parts of wheat germ oil, 1 part of ceramide 3, 9 parts of sodium hyaluronate and 40 parts of bifid yeast fermentation product lysate.

[0035] Example 4

[0036] A skin repair composition is obtained by uniformly mixing 38 parts of wheat germ oil, 3 parts of ceramide 3, 1 part of sodium hyaluronate and 58 parts of bifid yeast fermentation product lysate.

[0037] Comparative Example 1

[0038] A composition is obtained by uniformly mixing 9 parts of ceramide 3, 9 parts of sodium hyaluronate and 82 parts of bifid yeast fermentation product lysate.

[0039] Comparative Example 2

[0040] A composition is obtained by uniformly mixing 31.5 parts of wheat germ oil, 6 parts of sodium hyaluronate and 62.5 parts of bifid yeast fermentation product lysate.

[0041] Comparative Example 3

[0042] A composition is obtained by uniformly mixing 31.5 parts of wheat germ oil, 6 parts of ceramide 3 and 62.5 parts of bifid yeast fermentation product lysate.

[0043] Comparative Example 4

[0044] A composition is obtained by uniformly mixing 75 parts of wheat germ oil, 12.5 parts of ceramide 3 and 12.5 parts of sodium hyaluronate.

[0045] Comparative Example 5

[0046] A composition is obtained by uniformly mixing 30 parts of olive oil, 5 parts of ceramide 3, 5 parts of sodium hyaluronate and 60 parts of bifid yeast fermentation product lysate.

[0047] Comparative Example 6

[0048] A composition is obtained by uniformly mixing 30 parts of wheat germ oil, 5 parts of ceramide 1, 5 parts of sodium hyaluronate and 60 parts of bifid yeast fermentation product lysate.

[0049] Comparative Example 7

[0050] A composition is obtained by uniformly mixing 30 parts of wheat germ oil, 5 parts of ceramide 3, 5 parts of PCA sodium and 60 parts of bifid yeast fermentation product lysate.

[0051] Comparative Example 8

[0052] A composition is prepared by uniformly mixing 30 parts of wheat germ oil, 5 parts of ceramide 3, 5 parts of sodium hyaluronate and 60 parts of lactic acid bacteria fermentation lysate.

[0053] Performance Testing

[0054] 1. Fibroblast scratch test

[0055] Experimental background

[0056] Fibroblasts are cells that are easily recognized by their unique spindle shape, a feature that distinguishes them from other tissue structural cells, such as epithelial cells. They are a diverse group of cells that play a multifaceted role in health and disease: they help define tissue architecture by producing the extracellular matrix material that surrounds cells, they assist in the functioning and positioning of other cell types, and following injury, they promote healing or drive inflammation and scarring. Fibroblasts are important for repairing varying degrees of cell degeneration, necrosis and tissue loss, as well as bone trauma, and their unique properties can help repair damaged skin.

[0057] The wound healing method is a simple method for measuring cell migration and repair ability. It is similar to the in vitro wound healing model. On a monolayer of adherent cells cultured in a culture dish or plate in vitro, a line is drawn in the central area of ​​cell growth with a micropipette tip or other hard objects, and the cells in the central part are removed. The cells are then cultured for the set time of the experiment, and the cell culture plate is removed to observe the growth and migration ability of the surrounding cells. The experiment usually requires setting up a normal control group and an experimental group. The experimental group is a group to which certain treatment factors, drugs, exogenous genes, etc. are added. The migration and repair ability of each group of cells can be determined by the differences in the repair ability of the scratch area between cells in different groups.

[0058] Experimental Principle

[0059] When trauma occurs, fibroblasts are regulated by cytokines and extracellular matrix, and they rapidly proliferate and migrate, form granulation tissue to fill the wound, secrete collagen, and ultimately participate in scar formation. Fibroblasts can be used as a cell model to study the repair ability of cosmetics. The effectiveness of the test substance in repair can be evaluated by comparing the degree of healing of fibroblast scratches after administration of the test substance and the negative control.

[0060] Experimental setup

[0061] Composition test: The sample was diluted with pure water to a solution with a volume fraction of 4%, and then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0062] Negative control: basic culture medium (cell activity test); incomplete culture medium (scratch test).

[0063] Positive control: basal culture medium.

[0064] Experimental methods

[0065] Cell activity test: 3T3 cells were plated in 96-well plates. After 24 hours, the culture medium was discarded and basal culture medium containing test samples at different concentrations was added. After 24 hours, OD490nm was detected by MTT method and the effect of test samples on 3T3 cell activity was analyzed by t-test.

[0066] Fibroblast scratch test: After fibroblasts were plated on a 6-well plate and cultured for 24 hours, a line was drawn in the middle of the 6-well plate with a sterile pipette tip to produce a scratch, and the culture medium was discarded. The basal culture medium (positive control), incomplete culture medium (negative control), and culture medium of the corresponding sample group were added respectively, and the cell healing was observed at 0h and 24h, and photos were taken.

[0067] Calculation formula

[0068]

[0069] Data analysis

[0070] Image-pro plus (IPP) image analysis software was used to quantitatively analyze the cell scratch images at 0h and 24h.

[0071] The compositions prepared in Examples 1-4 and Comparative Examples 1-8 were tested according to the above experimental method, and the results are shown in Table 1;

[0072] Table 1 Fibroblast scratch test results of the compositions prepared in Examples 1-4 and Comparative Examples 1-9

[0073]

[0074]

[0075] When the concentration of each test sample is 0.25%, the cell activity is greater than 90%. According to the results in Table 1:

[0076] The fibroblast scratch healing rate of the compositions of Examples 1-4 and Comparative Examples 1, 5 and 6 is significantly improved compared with that of the negative control group, indicating that these compositions have a promoting effect on the healing of fibroblast scratches, indicating that the compositions have a repairing effect; according to the component composition of each embodiment and comparative example, it can be obtained that when ceramide 3, sodium hyaluronate and bifida yeast fermentation product lysate are combined, they have a certain skin repairing effect, and the lack of any one of the three will greatly reduce the skin repairing effect.

[0077] According to the data comparison between Example 1 and Comparative Example 1, it can be seen that in the absence of wheat germ oil, the skin repair effect of the composition is significantly reduced, indicating that the present invention uses wheat germ oil with ceramide 3, sodium hyaluronate, and bifid yeast fermentation product lysate to produce a synergistic effect, which greatly improves the skin repair efficacy of ceramide, sodium hyaluronate, and bifid yeast fermentation product lysate.

[0078] Comparative Example 5 uses olive oil instead of wheat germ oil, and the fibroblast scratch healing rate obtained by the composition does not change much compared with that in Comparative Example 1, indicating that olive oil, which has similar efficacy to wheat germ oil, cannot produce a synergistic effect with ceramide 3, sodium hyaluronate, and bifida fermentation product lysate, and cannot achieve the effect of synergistically enhancing the skin repair effect.

[0079] Comparative Example 6 uses ceramide 1 instead of ceramide 3, and the fibroblast scratch healing rate obtained by the composition does not change much compared with that in Comparative Example 1, indicating that wheat germ oil can only synergistically enhance the skin repair effect of ceramide 3, sodium hyaluronate, and bifida fermentation product lysate, and in the absence of ceramide 3, wheat germ oil cannot exert the corresponding synergistic effect.

[0080] Similarly, according to the data comparison of Example 1 and Comparative Examples 7 and 8, it can be seen that the synergistic enhancement of the skin repairing effect of ceramide 3, sodium hyaluronate and bifid yeast fermentation product lysate by wheat germ oil is based on the compound of wheat germ oil, ceramide 3, sodium hyaluronate and bifid yeast fermentation product lysate. Replacing any one of the components will result in the synergistic enhancement effect produced by it not being exerted, indicating that the present invention adopts wheat germ oil, ceramide 3, sodium hyaluronate and bifid yeast fermentation product lysate to produce a synergistic enhancement of skin repairing effect.

[0081] According to the comparison between Example 1 and Comparative Examples 2-4, it can be seen that the composition of the present invention, which is compounded with wheat germ oil, ceramide 3, sodium hyaluronate, and bifida yeast fermentation product lysate, is difficult to exert significant skin repairing effects if any one of the components is indispensable.

[0082] 2. Human body repair efficacy test

[0083] Recruitment of subjects

[0084] It is planned to enroll 100 qualified subjects and ensure that at least 90 subjects will be completed in the end. At least 30 of them will complete the test of the moisture content of the skin stratum corneum of the essence containing different concentrations of the composition of Example 1, and the test of the change of the moisture content of the skin of the essence containing 3% of the composition of Examples 1-4 and Comparative Examples 1-9.

[0085] 1) Chinese men or women who are in good health

[0086] 2) Age: 18 to 60 years old (excluding pregnant or lactating women)

[0087] 3) The test site has not participated in other clinical trials at present or in the past three months

[0088] 4) No serious systemic diseases, no immunodeficiency or autoimmune diseases, and the tested area has not received skin treatment, cosmetic treatment or other tests that may affect the results

[0089] 5) Those without active allergic diseases

[0090] 6) Those who are not highly sensitive

[0091] 7) Those who have not used hormone drugs or immunosuppressants in the past month

[0092] 8) Volunteer to participate in the trial and willing to sign the informed consent form

[0093] 9) Does not violate exclusion criteria and can comply with restrictions

[0094] Human body repair test method

[0095] Before using the sample (0h): the subjects cleaned the inside of both arms and sat quietly at the required temperature for 20 minutes. During this period, they were not allowed to walk or eat. The test site was exposed and they remained relaxed. The test area was marked. The area of ​​the test area was at least 3cm*3cm, and the interval was at least 1cm. The sample area and the control area were randomly allocated according to the blind table. The skin barrier of the test area and the control area was damaged by tearing with low-allergenic tape. The staff used a skin moisture loss meter (TewameterTM300) and a skin moisture tester (Corneometer CM825) to measure the baseline value of the test site of the subject, and then the staff measured the test area according to (2.0±0.1)mg / cm 2 The blank control group samples were applied evenly at a dosage of (2.0±0.1) mg / cm 2 Apply evenly in a single application.

[0096] After using the sample (0.5h, 1h, 2h): the same laboratory technician measured the transepidermal water loss rate and moisture content of the test area of ​​the subject and recorded the data.

[0097] Instruments and Equipment

[0098] Table 2 Test instrument information

[0099] Instrument Name Model Specifications Skin water loss meter Tewameter TM300 Electronic balance Practum 224-1CN Skin Moisture Tester Corneometer CM825

[0100] Test Results

[0101] SPSS software was used to perform descriptive statistics on each test value. The Shapiro-Wilk Test was used to test the significance of the normal distribution of the data change value. If P>0.05, it was normally distributed, and the paired sample t test was performed, with a significant difference level of α=0.05. If P<0.05, it was non-normally distributed, and the Wilcoxon signed rank test was performed.

[0102] 2.1 Testing of the human body repair efficacy of the essence containing different concentrations of the composition of Example 1

[0103] According to the essence formula in Table 3, essences containing the composition of Example 1 at different concentrations were prepared, and the amount of each substance used was expressed by mass fraction.

[0104] Table 3 Essence formula containing different concentrations of the composition of Example 1

[0105]

[0106] The essence prepared in Table 3 was tested according to the above test method, and the results are shown in Table 4 and Table 6;

[0107] Table 4 Test results of moisture content of the stratum corneum of the essence containing different concentrations of the composition of Example 1

[0108]

[0109]

[0110] Samples 1-5 in Table 4 were compared with the blank control group, and the results are shown in Table 5;

[0111] Table 5 Comparative analysis of the test results of skin stratum corneum moisture content of samples 1-5 and the blank control group

[0112]

[0113] According to the results in Table 4 and Table 5, we can see that:

[0114] According to the test results in Table 4, each sample will gradually alleviate the decrease in skin moisture content caused by tearing of the hypoallergenic tape over time, but the blank control group and sample 1 did not have a significant increase in skin moisture content after use. The moisture content of sample 2 at T3 (2h) was significantly different from the initial value. Sample 3 had a significant increase in moisture content at T2 (1h) and T3 (2h) relative to the initial moisture content (the average value of the test just after tearing off). Samples 4 and 5 had a significant increase in moisture content from T1 (30min) to T3 (2h) relative to the initial value.

[0115] According to the data analysis results in Table 5, by comparing the blank control and the sample change rates of each concentration gradient, it can be seen that: the skin moisture content change rate of sample 1 in the three time periods of T0 (0h)-T3 (2h) is not significantly different from the change rate of the blank control; the mean change rate of sample 2 tested at T3 (2h) is significantly different from the water content change rate of the blank control at T3 (2h), but there is no significant difference between the two groups in other time periods; the skin moisture content of samples 3, sample 4 and sample 5 in the three time periods of T0 (0h)-T3 (2h) is significantly different from the water content change rate of the blank control at T3 (2h). There are significant differences in the rate of change of water content between the two groups relative to the rate of change of the blank control; this indicates that compared with the rate of change of water content of the blank control, the skin care products containing 0.5% of the composition of the present invention do not show obvious effect of helping the skin to restore water content, and from the time the skin care products contain 1.0% of the composition of the present invention, they begin to show significant effect of helping the skin to restore water content, and in the range of 1.0-3.0%, as the concentration gradually increases, the restoration effect becomes more significant, and although the composition with a concentration greater than 3.0% has significant efficacy, the increase in concentration is of little significance.

[0116] Table 6 Results of transepidermal water loss test of essence containing different concentrations of the composition of Example 1

[0117]

[0118]

[0119] The samples 1-5 in Table 6 and the blank control group were compared and analyzed, and the results are shown in Table 7;

[0120] Table 7 Comparative analysis of skin transepidermal water loss test results of samples 1-5 and blank control group

[0121]

[0122] According to the test results in Table 6, with the passage of time, the transepidermal water loss of the skin caused by tearing of the low-allergenic tape will be alleviated after applying each sample. Among them, the change rate of the blank control measured at T1 (30min) has no significant difference, and the change rate measured at T2 (1h) and T3 (2h) relative to the initial value T0 (0h) has a significant difference. Samples 1-5 have a significant effect of reducing transepidermal water loss from T1 (30min) to T3 (2h) relative to the initial value.

[0123] According to the data analysis results in Table 7, by comparing the blank control and the sample change rates of each concentration gradient, it can be seen that: the skin transepidermal water loss change rate of sample 1 in the three time periods of T0 (0h)-T3 (2h) is not significantly different from the change rate of the blank control; the mean change rate of sample 2 tested at T3 (2h) is significantly different from the transepidermal water loss change rate of the blank control at T3 (2h), but there is no significant difference between the two groups in other time periods; the mean change rate of sample 2 tested at T2 (1h) and T3 (2h) is significantly different from the mean change rate of the blank control tested at T2 (1h) and T3 (2h). There was no significant difference in the change rate at T1 (30min) between the two groups; the change rates of transepidermal water loss of samples 4 and 5 in the three time periods of T0 (0h)-T3 (2h) relative to the change rate of the blank control were significantly different between the two groups; this shows that compared with the transepidermal water loss change rate of the blank control, the 0.5% composition did not show a significant effect in helping to repair the skin barrier, and from 1.0% concentration it showed a significant effect in helping to repair the skin barrier, and as the concentration gradually increased from 1.0 to 3.0%, the recovery effect became more significant, and although the composition with a concentration greater than 3.0% had a significant effect, the increase in concentration was not significant.

[0124] Therefore, the most suitable content of the skin repair composition in the skin care product of the present invention is between 1.0-3.0%.

[0125] 2.2 Human body repair efficacy test of different compositions at the same concentration

[0126] According to the formula of sample 4 in Table 3, Examples 1-4 and Comparative Examples 1-8 were used to replace Example 1 to prepare essences containing 3% of the composition, and then tested according to the above human body repair test method. The results are shown in Tables 8 and 10;

[0127] Table 8 Test results of skin moisture content changes of essence containing 3% of the composition of Examples 1-4 and Comparative Examples 1-8

[0128]

[0129]

[0130] The data in Table 8 are compared and analyzed, and the results are shown in Table 9;

[0131] Table 9 Comparative analysis of skin moisture content changes of essence containing 3% of the composition of Examples 1-4 and Comparative Examples 1-8 and blank control

[0132]

[0133]

[0134]

[0135] According to Table 8, we can see that:

[0136] According to the data comparison of Examples 1-4 and the blank control group, the technical solution of the present invention using wheat germ oil, ceramide 3, sodium hyaluronate, and bifida fermentation product lysate can significantly alleviate the reduction of skin moisture content caused by tearing of the hypoallergenic tape.

[0137] According to the comparison of the data of comparative examples 1-4 and the blank control group, it can be seen that when one of the components of wheat germ oil, ceramide 3, sodium hyaluronate and bifid yeast fermentation product lysate is missing, there is no significant increase in skin moisture content after use of the compositions of comparative examples 1-4, indicating that the technical solution of compounding wheat germ oil, ceramide 3, sodium hyaluronate and bifid yeast fermentation product lysate of the present invention is indispensable, and the compounding of the four can produce the effect of synergistically improving the skin repair effect.

[0138] According to the comparison of the data of comparative examples 5-8 and the blank control group, it can be seen that there is no significant increase in skin moisture content after use of the compositions of comparative examples 5 and 8, indicating that wheat germ oil and bifid yeast fermentation product lysate play an important role in the technical scheme of the present invention, and their presence is to play the role of synergistically improving the skin repair effect produced by the combination of wheat germ oil, ceramide 3, sodium hyaluronate, and bifid yeast fermentation product lysate; while there is no significant increase in skin moisture content in a short period of time after use of comparative examples 6 and 7, and only at T3 (2h) there is a more obvious improvement in alleviating the decrease in skin moisture content caused by tearing of the hypoallergenic tape. The reason may be that wheat germ oil and bifid yeast fermentation product lysate can provide a better environment for the skin, and cooperate with other skin repair components to have a certain effect of increasing moisture content after a long time.

[0139] According to Table 9, we can see that:

[0140] By comparing the sample change rates of the blank control and the 3% concentration of each composition, it can be known that: that is, the skin moisture content change rates of comparative examples 1-9 in the three time periods of T0 (0h)-T3 (2h) relative to the change rate of the blank control, there is no significant difference between the two groups; the skin moisture content change rates of embodiments 1-4 in the three time periods of T0 (0h)-T3 (2h) relative to the change rate of the blank control, there are significant differences between the two groups; compared with the moisture content change rate of the blank control, comparative examples 1-9 do not show obvious effects of helping the skin to restore moisture content, while embodiments 1-3 have significant effects of helping the skin to restore moisture content, indicating that the present invention uses wheat germ oil, ceramide 3, sodium hyaluronate, and bifid yeast fermentation product lysate to produce a synergistic effect of enhancing the skin repair effect.

[0141] Table 10 Test results of skin transepidermal water loss changes of essence containing 3% of the composition of Examples 1-4 and Comparative Examples 1-8

[0142]

[0143]

[0144]

[0145] The data in Table 10 are compared and analyzed, and the results are shown in Table 11;

[0146] Table 11 Comparative analysis of skin transepidermal water loss changes of essence containing 3% of the composition of Examples 1-4 and Comparative Examples 1-9 and blank control

[0147]

[0148]

[0149] According to the results in Table 10:

[0150] Examples 1-4 and Comparative Examples 1-9 will gradually alleviate the problem of large transepidermal water loss caused by damaged skin barrier due to tearing of low-allergenic tape over time, but there is no significant difference in the change rate measured at T1 (30min) among Comparative Example 4, Comparative Example 8 and the blank control group, and there is a significant difference in the change rate measured at T2 (1h) and T3 (2h) relative to the initial value T0 (0h), indicating that when the bifid yeast fermentation product lysate is missing, the ability of the composition to reduce transepidermal water loss in a short time is poor, indicating that in the technical scheme of the present invention, the bifid yeast fermentation product lysate not only plays a synergistic role with wheat germ oil, ceramide 3, and sodium hyaluronate to enhance the skin repair effect, but also plays a role in accelerating the efficacy of the composition.

[0151] Although Examples 1-4 and Comparative Examples 1-9 all showed a certain effect of reducing transepidermal water loss, the difference in the effects between Examples 1-4 and Comparative Examples 1-9 was very obvious, indicating that the technical solution of the present invention also has a significant effect in alleviating transepidermal water loss caused by damaged skin barrier due to tearing of hypoallergenic tape.

[0152] According to the results in Table 11, we can see that:

[0153] According to the data comparison of Example 1 and Comparative Examples 1-3, it can be seen that in the absence of any one of the wheat germ oil, ceramide 3 and sodium hyaluronate of the present invention, the effect of the composition in alleviating the skin barrier damage caused by tearing of the hypoallergenic tape will be greatly reduced, indicating that the technical solution of the present invention using wheat germ oil, ceramide 3, sodium hyaluronate and bifida fermentation product lysate has the effect of synergistically improving the skin repair ability.

[0154] According to the data comparison between Example 1 and Comparative Example 4, although Comparative Example 4 has a more significant effect of alleviating the transepidermal water loss caused by the damaged skin barrier due to tearing of the hypoallergenic tape after T2 (1h), the effect it exhibits is obviously not as good as that of Example 1 of the present invention. The possible reason is that the compound combination of wheat germ oil, ceramide 3 and sodium hyaluronate exhibits a good moisturizing effect, thereby reducing water loss. However, compared with the synergistic skin repair effect produced by the compound of wheat germ oil, ceramide 3, sodium hyaluronate and bifida fermentation product lysate of the present invention, its ability to reduce water loss is weaker.

[0155] Similarly, according to the data of Comparative Examples 7 and 8, the compound combination of wheat germ oil and ceramide 3, sodium hyaluronate or sodium PCA exhibits good moisturizing effect, thereby reducing water loss, but it does not exert skin repair effect, so the ability to reduce water loss is significantly reduced compared to the technical solution of the present invention.

[0156] According to the data of Comparative Example 5, there is no significant difference between the two groups in the change rate of transepidermal water loss of Comparative Example 5 in the three time periods of T0 (0h)-T3 (2h) relative to the change rate of the blank control, indicating that the technical solution of Comparative Example 5 does not show a significant effect in helping to restore the skin barrier.

[0157] According to the data of Comparative Example 6, the mean change rate of Comparative Example 6 tested at T3 (2h) is significantly different from the transepidermal water loss change rate of the blank control tested at T3 (2h), but there is no significant difference between the two groups in other time periods, indicating that the technical solution of Comparative Example 6 has a certain skin repair effect after a long time at T3 (2h), but it is not as significant as the technical solution of the present invention. It is speculated that ceramide 1 may be not as good as ceramide 3 in synergistic enhancement.

[0158] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A skin repair composition, characterized in that: The invention is composed of the following components by weight percentage: 30-50% wheat germ oil, 1-5% ceramide 3, 1-10% sodium hyaluronate, and 40-60% bifida yeast fermentation product lysate.

2. The skin repair composition according to claim 1, characterized in that The composition is as follows by weight: 30% wheat germ oil, 5% ceramide 3, 5% sodium hyaluronate, and 60% bifida yeast fermentation product lysate.

3. The skin repair composition according to claim 1, characterized in that By weight percentage, it is composed of the following components: 39% wheat germ oil, 1% ceramide 3, 10% sodium hyaluronate, and 50% bifida yeast fermentation product lysate.

4. The skin repair composition according to claim 1, characterized in that By weight percentage, it is composed of the following components: 50% wheat germ oil, 1% ceramide 3, 9% sodium hyaluronate, and 40% bifida yeast fermentation product lysate.

5. A skin care product, characterized in that: The invention comprises the skin repair composition according to any one of claims 1 to 4, wherein the content of the skin repair composition is 1 to 4%.

6. The skin care product according to claim 5, characterized in that The content of the skin repair composition in the skin care product is 1-3%.

Images