Method for solid-phase synthesis of gnrh castration vaccine
A technology for solid-phase synthesis and castration vaccine, which is applied in chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of poor product quality and high impurity content, and achieve the effect of separation
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Embodiment 1
[0067] The GnRH peptide chain in this example is: p-Glu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-Gly-Gln-His-Trp-Ser-Tyr-D-Ala-Leu-Arg -Pro-Gly-Cys-Lys-NH 2 .
[0068] In this embodiment, the GnRH parallel body and the vaccine synthesis method are as follows:
[0069]
[0070] Synthesis of GnRH Conjugates
[0071] Using MBHA resin as the carrier resin, dichloromethane was swelled for more than 3 hours, and coupled with Fmoc-Lys(BOC)-OH, and Lys was coupled to MBHA resin to obtain Fmoc-Lys(BOC)-MBHAResin. The method is: react 3eq of Fmoc-Lys(Fmoc)-OH with 3eqHOBT, 3eq of DIC and 1.5eq of NMM, the reaction solvent is anhydrous DMF, and the reaction time is 1.5h.
[0072] In the presence of 20% piperidine and DMF, the protecting group Fmoc of Lys was removed to give NH 2 -Lys-MBHA Resin.
[0073] In the presence of catalyst and coupling agent DIC+NMM+HOBT, Fmoc-rinkamidelinker is coupled to the two side chains of Lys respectively, the method is: 6eq of Fmoc-Rinkamidlinker-OH a...
Embodiment 2
[0086] In this embodiment, the GnRH peptide chain is:
[0087] p-Glu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-Lys-Gln-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-Lys-Cys-Gly- NH 2
[0088] In this embodiment, the GnRH parallel body and the vaccine synthesis method are as follows:
[0089] Synthesis of GnRH Conjugates
[0090] Using MBHA resin as a carrier resin, carry out coupling reaction with Fmoc-Lys(BOC)-OH, and couple Lys to MBHA resin to obtain Fmoc-Lys(BOC)-MBHAResin. In the presence of 20% piperidine and DMF, the protecting group of Lys was removed to give NH 2 -Lys-MBHA Resin.
[0091] In the presence of catalyst and coupling agent DIC+NMM+HOBT, Fmoc-rinkamidelinker is coupled to the two side chains of Lys respectively, and then the protecting group is removed to obtain:
[0092]
[0093] Gly and Cys were coupled sequentially, and then other amino acids were sequentially coupled according to the above peptide chain structure in this example, so as to realize the simultaneous ...
Embodiment 3
[0102] In this embodiment, the GnRH peptide chain is:
[0103] p-Glu-His-Trp-Ser-Tyr-D-Lys-Leu-Arg-Pro-Gly-Gln-His-Trp-Ser-Tyr-D-Lys-Leu-Arg-Pro-Gly-Cys-Gly- NH 2
[0104] In this embodiment, the GnRH parallel body and the vaccine synthesis method are as follows:
[0105] Synthesis of GnRH Conjugates
[0106] Using MBHA resin as a carrier resin, carry out coupling reaction with Fmoc-Lys(BOC)-OH, and couple Lys to MBHA resin to obtain Fmoc-Lys(BOC)-MBHAResin. In the presence of 20% piperidine and DMF, the protecting group of Lys was removed to give NH 2 -Lys-MBHA Resin.
[0107] In the presence of catalyst and coupling agent DIC+NMM+HOBT, Fmoc-rinkamidelinker is coupled to the two side chains of Lys respectively, and then the protecting group is removed to obtain:
[0108]
[0109] Gly and Cys were coupled sequentially, and then other amino acids were sequentially coupled according to the above peptide chain structure in this example, so as to realize the simultaneou...
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