Control method of PCR continuous reaction

Abstract

The invention relates to a control method of a PCR continuous reaction, which comprises: a sample is injected into a pipeline layer through a sample adding hole, and a lysis solution is injected into the pipeline layer through a first reagent tube to form a product; the product is pulled out through a first piston connected with the first reagent tube, and is pushed into a purification bin along the pipeline; an ultrasonic unit is started, the ultrasonic unit is arranged below the purification bin, and the ultrasonic unit scatters the magnetic beads in the purification bin, so that nucleic acid substances in the product are adsorbed on the surfaces of the magnetic beads; a magnetic attraction unit is started, a magnet in the magnetic attraction unit is pushed to the position below the purification bin, and the magnetic beads are attracted; the cleaning liquid in a third reagent tube is pushed into the purification bin, and cleaning is conducted at least once; the nucleic acid substance is eluted to separate the nucleic acid substance from the magnetic beads; nucleic acid is pushed into an amplification bin in the pipeline layer; and the amplification reaction is realized. According to the invention, the control on the continuous PCR reaction is realized, the pipeline integration degree in the reaction process is relatively high, the operation is simple, and the realization is easy.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for controlling continuous PCR reactions. Background technique [0002] The extraction, purification, and amplification of nucleic acid are routine operations in nucleic acid detection tests, and are also critical steps in the process of gene analysis. In the process of gene analysis, it is often necessary to separate specific nucleic acid fragments from mixed samples for subsequent PCR amplification. Therefore, the purification and recovery of nucleic acid directly affects the progress and results of the entire gene analysis process. [0003] The Chinese patent discloses a nucleic acid automatic detection device, which includes a fixed base and a main support plate vertically fixed on the fixed base, an automatic sample reaction mechanism and its fixed frame, a nucleic acid detection optical path structure and its supporting frame, and a controller; The automatic me...

AI Technical Summary

Problems solved by technology

[0004] In the above technical solutions, on the one hand, the detection device has high environmental requirements and low test accuracy. At the same time, the internal pipeline of the chip is not integrated, and it i

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