Method for preparing active polypeptides from deep-sea fish meat
A technology of active peptides and deep-sea fish, applied in the direction of microorganism-based methods, biochemical equipment and methods, peptide sources, etc., can solve the problems of poor competitiveness, low added value of products, single product structure, etc., and achieve the goal of improving stability Effect
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Embodiment 1
[0021] The method for preparing an active polypeptide by utilizing deep-sea fish meat comprises the following steps:
[0022] 1) Degreasing: cut deep-sea fish meat into small pieces, add 2~4‰ chitosan immobilized lipase, add 8~15 times the volume of distilled water, and enzymolyze at a constant temperature of 25~50℃ for 0.8~2.5h, and enzymolyze After the end, the enzyme is inactivated, and the defatted deep-sea fish meat is obtained by filtration. The preparation steps of chitosan-immobilized lipase are as follows: use dry chitosan powder, soak overnight with 60-80mmol / L phosphate buffer solution of pH 6.0-6.8, then add 0.7-1.2% chitosan powder mass 1-ethyl-3-(3-methylamino)propylcarbodiimide was activated for 0.5~0.9h, then chitosan powder 1~2 times the volume of glutaraldehyde coupling reaction was added for 1~1.8h, with Wash with distilled water for 5-8 times, add 0.7-2.0mg / mL lipase solution and 0.1-0.3‰ active short peptide, stir slowly in an ice bath for 0.5-1.5h, then ...
Embodiment 2
[0030] The most preferred method for preparing active polypeptides from deep-sea fish meat comprises the following steps:
[0031] 1) Degreasing: cut deep-sea fish meat into small pieces, add 3.2‰ chitosan immobilized lipase, add 10 times the volume of distilled water, and enzymolyze at a constant temperature of 30°C for 1.4 hours. Deep-sea fish meat defatted. The preparation steps of chitosan-immobilized lipase are: use dry chitosan powder, soak overnight with 65mmol / L phosphate buffer solution of pH 6.3, then add 1.0% 1-ethyl-3 -(3-Methylamino)propyl carbodiimide activation for 0.7h, then add chitosan powder 1.4 times the volume of glutaraldehyde coupling reaction for 1.3h, wash with distilled water 5 times, add 1.2mg / mL lipase Solution and 0.3‰ active short peptide were slowly stirred in an ice bath for 1.1h, then placed in the refrigerator overnight, rinsed thoroughly with distilled water the next day, filtered and dried to obtain chitosan-immobilized lipase. The amino a...
Embodiment 3
[0039] The method for preparing an active polypeptide by utilizing deep-sea fish meat comprises the following steps:
[0040] 1) Degreasing: Deep-sea fish meat is cut into small pieces, added with 3‰ chitosan immobilized lipase, added 11 times the volume of distilled water, and enzymatically hydrolyzed at a constant temperature of 30°C for 1.2 hours. Deep-sea fish meat defatted. The preparation steps of chitosan-immobilized lipase are: use dry chitosan powder, soak overnight with 70mmol / L phosphate buffer solution of pH6.5, then add 1-ethyl-3 -(3-Methylamino)propyl carbodiimide activation for 0.8h, then add chitosan powder 1.3 times the volume of glutaraldehyde coupling reaction for 1.2h, wash with distilled water 6 times, add 1.3mg / mL lipase Solution and 0.2‰ active short peptide were slowly stirred in an ice bath for 1.0 h, then placed in the refrigerator overnight, rinsed thoroughly with distilled water the next day, filtered and dried to obtain chitosan-immobilized lipase...
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