The invention relates to the technical field of anti-fatigue
peptide preparation, and discloses an anti-fatigue
peptide preparation method, which comprises: 1,
raw material pretreatment: selecting fresh
deep sea fish skin, washing, dicing, carrying out
freeze drying, and carrying out
superfine grinding under
nitrogen protection to obtain
fish skin micro
powder; step 2, preparation of a composite enzymatic
hydrolysate: mixing
trypsin,
papain and bromelain according to a ratio, and adding the mixture into a
phosphoric acid buffer solution to prepare the composite enzymatic
hydrolysate; a compound
enzyme and ultrasonic wave synergistic enzymolysis process is adopted, the synergistic effect of the compound
enzyme is combined with assistance of ultrasonic
waves, full contact of the
enzyme and a substrate is promoted, the enzymolysis efficiency is greatly improved, and compared with a traditional single enzymolysis method or a non-ultrasonic-assistance method, the yield of the anti-fatigue
peptide is improved by 20% or above; meanwhile, through a macroporous adsorption resin and
ultrafiltration membrane two-step purification combination technology, impurities are effectively removed, the product purity is improved by 30% or above, the molecular
weight distribution is more uniform, and the product quality is improved.