The invention belongs to the technical field of
fatty acid extraction, and particularly relates to a method for extracting high-quality
fatty acid from deep-sea fish and application of the high-quality
fatty acid to preparation of pet fur health-care products, the extraction method comprises the following steps:
raw material pretreatment, preliminary enzymolysis, deep enzymolysis,
urea impurity removal and multi-stage refining; according to the prepared fatty acid, the measured extraction rate of the fatty acid is 95.3-99.3%, the extraction rate is high, after the
fullerene-loaded
lipase is oscillated for 10 h in a water tank at the speed of 80 r / min, the residual
enzyme activity is 80.3%, the firmness degree of combination of zymoprotein and a
fullerene carrier is high, the
fullerene-loaded
lipase can be repeatedly used, the
enzyme activity is 50.1% after twenty times of recycling, and the fatty acid can be used for preparing the fatty acid. The method has the advantages that the
utilization rate of the
enzyme is high, the measured
acid value of the fatty acid is 0.066-0.076 mg / g, the
peroxide value is 0.990-1.102 mol / kg, the fatty acid is free of heavy
metal ions, the DHA content is 41.25-45.87%, the color is bright yellow, the fatty acid is clear and free of fishy smell, and the quality of the fatty acid is high.