Feed additive containing marine organism extraction components and preparation method thereof

Abstract

The invention provides a compound feed additive containing marine organism extraction components, a preparation method of the compound feed additive and application of the compound feed additive in quail breeding. The additive is prepared from natural components such as a seaweed extract, deep sea fish oil, coral calcium powder, astaxanthin, a marine microorganism fermentation product, a kelp extract, pearl powder and marine collagen peptide through a specific process. The preparation method of the additive comprises the following steps: uniformly mixing all the components under specific conditions, and then drying to specified moisture content through a spray drying method. The additive is rich in marine bioactive components, can comprehensively meet the nutritional requirements of quails, and improves the growth performance and immunity of the quails. In the quail feeding process, 6-10 kg of the additive is added into each ton of basal feed, and the laying rate, egg quality and growth speed of quails can be remarkably improved. The invention provides an efficient and environment-friendly feed additive for the quail breeding industry, and the feed additive has a wide market application prospect.

Description

Technical Field

[0001] The invention belongs to the field of new materials, and specifically discloses a feed additive containing marine biological extraction components and a preparation method thereof. Background Art

[0002] In the existing technology, feed additives, as an indispensable part of animal husbandry and aquaculture, play an important role in improving animal growth performance, enhancing immunity, improving product quality, etc. However, traditional feed additives have many shortcomings in terms of ingredients, functions and processing technology, which restricts their further development and application.

[0003] Existing feed additives are mainly divided into two categories: nutritional additives and non-nutritional additives. Nutritional additives mainly include amino acids, vitamins, minerals, etc., which are used to supplement or balance the nutrients in feed; non-nutritional additives include antibiotics, enzyme preparations, antioxidants, etc., which are used to prevent diseases, improve feed utilization and extend feed shelf life.

[0004] Although the existing technology has made some progress in the field of feed additives, it still has many shortcomings, which are specifically manifested in the following aspects:

[0005] Single ingredients and limited functions: Many traditional feed additives have single ingredients and can only meet certain nutritional needs of animals or solve a specific problem, and cannot comprehensively improve the growth performance and health level of animals.

[0006] Backward processing technology: In the preparation of marine biological extracts, traditional processing technology often has problems such as low extraction efficiency, large loss of active ingredients, and unstable product quality. For example, extreme conditions such as high temperature and high pressure can easily cause the active ingredients to denature or inactivate, affecting the effect of additives.

[0007] Lack of comprehensive application: In the existing technology, various feed additives are often used alone, lacking research on comprehensive application. The interaction and synergistic effect between different additives have not been fully explored and utilized, limiting their overall effect.

[0008] Impact on the environment and animal health: Long-term use of some traditional feed additives such as antibiotics may lead to the production of drug-resistant bacteria in animals, affecting animal health; at the same time, the discharge of these additives may also cause environmental pollution.

[0009] High cost: As a new feed additive, the preparation process of marine biological extracts is complicated and the source of raw materials is limited, resulting in high costs and difficulty in large-scale promotion and application in the aquaculture industry. Summary of the invention

[0010] In view of the deficiencies of the prior art, the present invention provides a feed additive containing marine biological extract ingredients and a preparation method thereof.

[0011] The present invention includes the following technical solutions:

[0012] A composite feed additive with marine biological extraction ingredients, the additive is composed of the following natural ingredients in parts by weight:

[0013]

[0014] Furthermore, in the above-mentioned composite feed additive with marine biological extract ingredients, the seaweed extract is prepared by the following steps: using seaweed as raw material, after washing, drying, and crushing into powder, adopting water distillation or ultrasonic assisted extraction, stirring and extracting at 40-60°C overnight, filtering the filtrate with gauze, concentrating and vacuum drying at a low temperature of 60-70°C to obtain a powdered seaweed extract.

[0015] Furthermore, in the above-mentioned composite feed additive with marine biological extract ingredients, the coral calcium powder is prepared by the following steps: collecting natural corals, crushing, screening, and washing them, treating them with strong alkali to remove impurities, and then calcining and grinding them to obtain high-purity coral calcium powder.

[0016] Furthermore, in the above-mentioned composite feed additive containing marine biological extract ingredients, the marine microbial fermentation product is prepared by the following steps:

[0017] 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract;

[0018] 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract;

[0019] 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 50-60°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

[0020] Furthermore, in the above-mentioned composite feed additive with marine biological extract ingredients, the pearl powder is prepared by the following steps: selecting high-quality seawater pearls, after washing and drying, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 100-200 mesh sieve to obtain pearl powder.

[0021] Furthermore, in the above-mentioned composite feed additive containing ingredients extracted from marine organisms, the marine collagen peptide is prepared by the following steps:

[0022] Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

[0023] The present invention also discloses a preparation method of the composite feed additive, comprising the following steps: uniformly mixing the prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide under the conditions of a stirring speed of 50-100 rpm and a heating temperature not exceeding 60° C., and then drying by a spray drying method under the conditions of an inlet temperature of 40-60° C. and an outlet temperature of 50-65° C. to a moisture content of less than 5%, so as to obtain a composite feed additive with marine biological extract components.

[0024] The invention also discloses the use of the compound feed additive in quail breeding, wherein the quails are quails in the egg-laying period over 45 days old.

[0025] Furthermore, the above use includes the following steps: adding 6-10 kg of a composite feed additive containing marine biological extracts per ton of quail basic feed, and using a feed mixing device to fully mix the additive with the quail basic feed so that the additive is evenly distributed in the feed.

[0026] Furthermore, in the above use, the basic feed for quail comprises the following ingredients: 51wt% corn, 3.5wt% wheat bran, 39.7wt% soybean meal, 2.5wt% rapeseed cake, and 3.3wt% fish meal.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The marine biological extract compound feed additive introduced by this technical solution brings together a variety of natural ingredients such as seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptides. These ingredients are carefully selected and scientifically proportioned to provide comprehensive, balanced and efficient nutritional supplements for farmed animals such as quail. During the preparation process, we use advanced extraction, fermentation, drying and mixing technologies to ensure that each ingredient can maintain its optimal purity and biological activity. By strictly controlling various parameters in the preparation process, such as stirring speed, heating temperature, etc., we achieve uniform mixing of various components, thereby greatly improving the overall quality and stability of the product. This compound feed additive is not only rich in nutrients such as protein, amino acids, vitamins, minerals, etc., it can significantly improve the egg production rate and egg quality of farmed animals such as quail, and bring higher economic benefits to farmers. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Comparison of the average egg production (pieces) per quail per month in the test case;

[0030] Figure 2 Comparison of the average mass (g) of quail eggs per month in the test case. DETAILED DESCRIPTION

[0031] A composite feed additive with marine biological extraction ingredients, the additive is composed of the following natural ingredients in parts by weight:

[0032] 20-40 parts of seaweed extract

[0033] 10-20 servings of deep sea fish oil

[0034] Coral calcium powder 5-15 parts

[0035] Astaxanthin 3-10 parts

[0036] 5-12 servings of marine microbial fermentation products

[0037] 2-8 servings of kelp extract

[0038] 1-5 parts pearl powder

[0039] Marine collagen peptides 2-6 servings.

[0040] The seaweed extract is prepared by the following steps: seaweed is used as a raw material, after being washed, dried and crushed into powder, an ultrasonic-assisted extraction method is used, extraction is carried out overnight at 50° C., the filtrate is filtered through gauze, concentrated and vacuum-dried at 65° C. to obtain a powdered seaweed extract.

[0041] The coral calcium powder is prepared by the following steps: natural corals are collected, crushed, screened, and cleaned, then treated with a strong alkali to remove impurities, and then calcined and ground to obtain high-purity coral calcium powder.

[0042] The marine microbial fermentation product is prepared by the following steps:

[0043] 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract;

[0044] 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract;

[0045] 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 55°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

[0046] The pearl powder is prepared by the following steps: selecting high-quality seawater pearls, washing and drying them, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 200-mesh sieve to obtain the pearl powder.

[0047] The marine collagen peptide is prepared by the following steps:

[0048] Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

[0049] The preparation method of the above-mentioned composite feed additive comprises the following steps: mixing the prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide evenly under the conditions of a stirring speed of 75 rpm and a heating temperature not exceeding 60°C, and then drying by a spray drying method under the conditions of an inlet temperature of 50°C and an outlet temperature of 60°C to a moisture content of less than 5%, thereby obtaining a composite feed additive with marine biological extract ingredients.

[0050] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] Deep-sea fish oil was purchased from Jinan Qingan Chemical Co., Ltd. (Pet feed additive salmon oil)

[0052] Kelp extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. (kelp concentrated powder).

[0053] Example 1

[0054] A composite feed additive with marine biological extraction ingredients, the additive is composed of the following natural ingredients in parts by weight:

[0055] 20 parts of seaweed extract

[0056] Deep sea fish oil 10 servings

[0057] 5 portions of coral calcium powder

[0058] 3 servings of Astaxanthin

[0059] 5 servings of marine microbial fermentation products

[0060] 2 parts of kelp extract

[0061] 1 part pearl powder

[0062] Marine collagen peptides 2 servings.

[0063] The seaweed extract is prepared by the following steps: seaweed is used as a raw material, after being washed, dried and crushed into powder, an ultrasonic-assisted extraction method is used, extraction is carried out overnight at 50° C., the filtrate is filtered through gauze, concentrated and vacuum-dried at 65° C. to obtain a powdered seaweed extract.

[0064] The coral calcium powder is prepared by the following steps: natural corals are collected, crushed, screened, and cleaned, then treated with a strong alkali to remove impurities, and then calcined and ground to obtain high-purity coral calcium powder.

[0065] The marine microbial fermentation product is prepared by the following steps:

[0066] 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract;

[0067] 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract;

[0068] 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 55°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

[0069] The pearl powder is prepared by the following steps: selecting high-quality seawater pearls, washing and drying them, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 200-mesh sieve to obtain the pearl powder.

[0070] The marine collagen peptide is prepared by the following steps:

[0071] Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

[0072] The preparation method of the above-mentioned composite feed additive comprises the following steps: mixing the prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide evenly under the conditions of a stirring speed of 75 rpm and a heating temperature not exceeding 60°C, and then drying by a spray drying method under the conditions of an inlet temperature of 50°C and an outlet temperature of 60°C to a moisture content of less than 5%, thereby obtaining a composite feed additive with marine biological extract ingredients.

[0073] Example 2

[0074] A composite feed additive with marine biological extraction ingredients, the additive is composed of the following natural ingredients in parts by weight:

[0075]

[0076] The seaweed extract is prepared by the following steps: seaweed is used as a raw material, after being washed, dried and crushed into powder, an ultrasonic-assisted extraction method is used, extraction is carried out overnight at 50° C., the filtrate is filtered through gauze, concentrated and vacuum-dried at 65° C. to obtain a powdered seaweed extract.

[0077] The coral calcium powder is prepared by the following steps: natural corals are collected, crushed, screened, and cleaned, then treated with a strong alkali to remove impurities, and then calcined and ground to obtain high-purity coral calcium powder.

[0078] The marine microbial fermentation product is prepared by the following steps:

[0079] 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract;

[0080] 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract;

[0081] 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 55°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

[0082] The pearl powder is prepared by the following steps: selecting high-quality seawater pearls, washing and drying them, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 200-mesh sieve to obtain the pearl powder.

[0083] The marine collagen peptide is prepared by the following steps:

[0084] Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

[0085] The preparation method of the above-mentioned composite feed additive comprises the following steps: mixing the prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide evenly under the conditions of a stirring speed of 75 rpm and a heating temperature not exceeding 60°C, and then drying by a spray drying method under the conditions of an inlet temperature of 50°C and an outlet temperature of 60°C to a moisture content of less than 5%, thereby obtaining a composite feed additive with marine biological extract ingredients.

[0086] Example 3

[0087] A composite feed additive with marine biological extraction ingredients, the additive is composed of the following natural ingredients in parts by weight:

[0088] 40 parts of seaweed extract

[0089] Deep sea fish oil 20 servings

[0090] Coral calcium powder 15 parts

[0091] Astaxanthin 10 parts

[0092] 12 servings of marine microbial fermentation products

[0093] 8 portions of kelp extract

[0094] 5 parts pearl powder

[0095] Marine collagen peptides 6 servings.

[0096] The seaweed extract is prepared by the following steps: seaweed is used as a raw material, after being washed, dried and crushed into powder, an ultrasonic-assisted extraction method is used, extraction is carried out overnight at 50° C., the filtrate is filtered through gauze, concentrated and vacuum-dried at 65° C. to obtain a powdered seaweed extract.

[0097] The coral calcium powder is prepared by the following steps: natural corals are collected, crushed, screened, and cleaned, then treated with a strong alkali to remove impurities, and then calcined and ground to obtain high-purity coral calcium powder.

[0098] The marine microbial fermentation product is prepared by the following steps:

[0099] 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract;

[0100] 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract;

[0101] 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 55°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

[0102] The pearl powder is prepared by the following steps: selecting high-quality seawater pearls, washing and drying them, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 200-mesh sieve to obtain the pearl powder.

[0103] The marine collagen peptide is prepared by the following steps:

[0104] Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

[0105] The preparation method of the above-mentioned composite feed additive comprises the following steps: mixing the prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide evenly under the conditions of a stirring speed of 75 rpm and a heating temperature not exceeding 60°C, and then drying by a spray drying method under the conditions of an inlet temperature of 50°C and an outlet temperature of 60°C to a moisture content of less than 5%, thereby obtaining a composite feed additive with marine biological extract ingredients.

[0106] Comparative Example 1

[0107] Same as Example 2, but without the seaweed extract.

[0108] Comparative Example 2

[0109] Same as Example 2, except that the fermentation product of marine microorganisms is not contained.

[0110] Comparative Example 3

[0111] Same as Example 2, but without pearl powder and with marine collagen peptide.

[0112] Test Case

[0113] The compound feed additives of Examples 1-3 and Comparative Examples 1-3 were added to the basic feed of quail, with 8 kg of the compound feed additive containing marine biological extracts added to each ton of the basic feed of quail; the basic feed of quail included the following ingredients: 51 wt% corn, 3.5 wt% wheat bran, 39.7 wt% soybean meal, 2.5 wt% rapeseed cake, and 3.3 wt% fish meal.

[0114] Experimental design: 45-50 day old Korean female quails were randomly divided into 7 groups (6 experimental groups and 1 control group), 30 in each group. The experimental group was fed with the feed with additives, and the control group was fed with feed without additives. They were allowed to eat and drink freely. The monthly egg production was calculated from the second half of the month after feeding. The average value was calculated for three months, and the average weight of the eggs was calculated. The results are shown in Tables 1 and 2 and Figure 1-2 shown.

[0115] Table 1 Egg production

[0116]

[0117]

[0118] From the comparison between Examples 1-3 and the control group, the composite feed additive of the present invention can produce 4-5 more eggs per quail on average in the first to third months compared to no additive. From the comparison between Example 2 and Comparative Example 1, other conditions remain unchanged, seaweed extract is added, and each quail can produce 1-2.5 more eggs; from the comparison between Example 2 and Comparative Example 2, other conditions remain unchanged, marine microbial fermentation products are added, and each quail can produce 2-4 more eggs; from the comparison between Example 2 and Comparative Example 3, other conditions remain unchanged, pearl powder and marine collagen peptide are added, and each quail can also produce several more eggs.

[0119] Table 2 Quality of quail eggs

[0120]

[0121] From the comparison of Examples 1-3 and the control group, the use of the composite feed additive of the present invention, compared with no additive, in the first 1-3 months, the average mass of each quail egg can be increased by more than 15%. From the comparison of Example 2 and Comparative Example 1, other conditions remain unchanged, seaweed extract is added, and the mass of each quail can be increased by 10%; from the comparison of Example 2 and Comparative Example 2, other conditions remain unchanged, marine microbial fermentation products are added, and from the comparison of Example 2 and Comparative Example 3, other conditions remain unchanged, pearl powder and marine collagen peptides are added, and similar improvements can be obtained; comprehensively speaking, the marine biological extract component composite feed additive introduced by this technical solution brings together a variety of natural ingredients such as seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptides. These ingredients are carefully selected and scientifically proportioned to provide comprehensive, balanced and efficient nutritional supplements for farmed animals such as quails. In the preparation process, we use advanced extraction, fermentation, drying and mixing technologies to ensure that each ingredient can maintain its optimal purity and biological activity. By strictly controlling various parameters in the preparation process, such as stirring speed, heating temperature, etc., we achieve uniform mixing of various components, thereby greatly improving the overall quality and stability of the product. This compound feed additive is not only rich in nutrients, such as protein, amino acids, vitamins, minerals, etc., it can significantly improve the egg production rate and egg quality of farmed animals such as quails, bringing higher economic benefits to farmers.

[0122] The above are limited to several preferred embodiments of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A composite feed additive containing ingredients extracted from marine organisms, characterized in that: The additive consists of the following natural ingredients by weight: 20-40 parts of seaweed extract 10-20 servings of deep sea fish oil Coral calcium powder 5-15 parts Astaxanthin 3-10 parts 5-12 servings of marine microbial fermentation products 2-8 servings of kelp extract 1-5 parts pearl powder Marine collagen peptides 2-6 servings.

2. The compound feed additive containing marine biological extracts according to claim 1, characterized in that: The seaweed extract is prepared by the following steps: seaweed is used as a raw material, after being washed, dried and crushed into powder, water distillation or ultrasonic-assisted extraction is adopted, stirring extraction is carried out at 40-60° C. overnight, the filtrate is filtered through gauze, concentrated and vacuum-dried at a low temperature of 60-70° C. to obtain a powdered seaweed extract.

3. The composite feed additive containing marine biological extracts according to claim 1, characterized in that: The coral calcium powder is prepared by the following steps: natural corals are collected, crushed, screened, and cleaned, then treated with a strong alkali to remove impurities, and then calcined and ground to obtain high-purity coral calcium powder.

4. The composite feed additive containing marine biological extracts according to claim 1, characterized in that: The marine microbial fermentation product is prepared by the following steps: 1) The marine Bacillus S-12-86 strain was activated and cultured at 30° C. for 24 hours in a seed culture medium containing 5 g / L peptone, 3 g / L yeast extract, 5 g / L sodium chloride, 1 g / L potassium dihydrogen phosphate and an appropriate amount of bran extract; 2) Subsequently, 4% (v / v) of the activated bacterial solution was inoculated into a fermentation tank containing 10% (v / v) of a fermentation medium, the fermentation temperature was controlled to be 30° C., sufficient ventilation was performed, the stirring speed was 75 rpm, and the fermentation culture was carried out for 24 hours; during the fermentation process, the pH value was adjusted to a neutral range in a timely manner, and the generated foam was processed; the fermentation medium contained 10 g / L glucose, 5 g / L peptone, 5 g / L MgSO4·7H2O, 2 g / L CaCl2, 5 g / L sodium chloride, 1 g / L dipotassium hydrogen phosphate and an appropriate amount of bran extract; 3) After the fermentation is completed, the bacteria and impurities in the fermentation liquid are separated by a 15000g centrifugation method, and then the fermentation liquid is heated at 50-60°C for evaporation and concentration, and spray-dried to obtain a powdered marine microbial fermentation product.

5. The compound feed additive containing marine biological extracts according to claim 1, characterized in that: The pearl powder is prepared by the following steps: selecting high-quality seawater pearls, washing and drying them, grinding the pearls into fine powder by mechanical grinding or air flow crushing, and passing through a 100-200 mesh sieve to obtain the pearl powder.

6. The composite feed additive containing marine biological extracts according to claim 1, characterized in that: The marine collagen peptide is prepared by the following steps: Deep-sea fish are selected as raw materials. After descaling, deboning and de-eviscerating, collagen in the fish meat is extracted according to the existing process, and then the collagen is hydrolyzed into small molecule peptides by enzymatic hydrolysis. Marine collagen peptides are obtained after filtering, purification, concentration and drying. In the enzymatic hydrolysis method, effective concentrations of pepsin, trypsin, papain and alkaline protease are added to the enzymatic hydrolysis solution.

7. The method for preparing the compound feed additive according to any one of claims 1 to 6, characterized in that: The following steps are involved: The prepared seaweed extract, deep-sea fish oil, coral calcium powder, astaxanthin, marine microbial fermentation products, kelp extract, pearl powder and marine collagen peptide are mixed evenly under the conditions of a stirring speed of 50-100 rpm and a heating temperature not exceeding 60°C, and then dried by a spray drying method under the conditions of an inlet temperature of 40-60°C and an outlet temperature of 50-65°C to a moisture content of less than 5%, to obtain a composite feed additive with marine biological extract ingredients.

8. Use of the compound feed additive according to any one of claims 1 to 6 in quail breeding, wherein the quails are egg-laying quails over 45 days old.

9. The use according to claim 8, characterized in that The following steps are involved: Add 6-10 kg of compound feed additives containing marine biological extracts to every ton of quail basic feed. Use feed mixing equipment to fully mix the additives with the quail basic feed so that the additives are evenly distributed in the feed.

10. The use according to claim 9, characterized in that The basic feed for quail comprises the following ingredients: 51 wt% corn, 3.5 wt% wheat bran, 39.7 wt% soybean meal, 2.5 wt% rapeseed cake, and 3.3 wt% fish meal.

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