Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue

Abstract

The invention provides a full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue, used for field detection of pesticide residue based on enzyme inhibition reaction. The full-automatic and high-throughput micro-fluidic chip system is mainly composed of a portable analytical detector and a disposable micro-fluidic chip; the plastic polymer material of the micro-fluidic chip is manufactured through existing micro-machining technology; the micro-fluidic chip is composed of a central clamping groove, an extraction chamber, a sample chamber, a reaction chamber, a detection chamber, a micro-groove, a micro-hole and a quality control bar code; reagents (comprising an extracting solution, enzyme, a colour developing agent and the like) required for detecting pesticide residue are fixed in the micro-fluidic chip; the functions of sampling automatically and quantitatively, distributing a fluid, carrying out biochemical reaction and molecular recognition and the like can be realized; a fluid operation and control module, a photoelectric detection module, a data analyzing and processing module and an information storage and communication module are integrated in the portable analytical detector; operation and control of biochemical reaction in the micro-fluidic chip and rapid analysis and detection of to-be-detected indexes and pesticide residue can be realized; and thus, the micro-fluidic chip system and the method provided by the invention are particularly applied to full-automatic and high-throughput detecting and screening of pesticide residue in the samples, such as fruits, vegetables, soil and water.

Description

technical field [0001] The invention relates to the field of microfluidic chip analysis, in particular to a microfluidic chip system and method for fully automatic and high-throughput pesticide residue detection based on enzyme inhibition reactions. Background technique [0002] Enzyme inhibition reaction and analysis method have extremely in-depth research and extremely wide application in the field of detection of toxic and harmful substances such as heavy metal ions, organophosphorus and carbamate pesticides. For example, at present, methods and instruments for rapid detection of pesticide residues based on the principle of enzyme inhibition (mainly including rapid detection instruments for pesticide residues and rapid detection cards for pesticide residues) have been included in national standards and industry standards (GB / T5009.199-2003) , and widely used in vegetable, fruit, tea and other production bases, farmers' markets, supermarkets and supervision departments, has...

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Problems solved by technology

[0003] However, the existing rapid detection products for pesticide residues are often based on traditional biochemical analysis laboratory detection methods to achieve enzyme inhibition reaction analysis and detection

For example, the existing pesticide residue rapid detector mainly relies on a large number of accessories such as pipettes, beakers, cuvettes and analysis vessels to complete the enzyme inhibition analysis. substances), enzyme inhibition reaction, and color reaction process are cumbersome, all rely on manual completion, which takes a long time, and the operator needs to have certain professional knowledge, which can only be carried out in the laboratory, and it is difficult to achieve rapid on-site detection; in order to achieve rapid on-site detection People have also developed a quick test card for pesticide residues based on the principle of enzyme inhibition reaction. Although this method is simple and easy to implement, this method has disadvantages such as poor reaction uniformity and low analysis accuracy. Immobilize enzymes on the surface of porous solid media such as test paper, and then add the solution sample dropwise on the surface of the test paper to complete the enzyme inhibition reaction process. Big

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