A microfluidic-based enzyme inhibition reaction platform and analysis method

Abstract

The invention provides a microfluidic-based enzyme inhibition reaction platform and analysis method, using the negative pressure spontaneously generated in the microfluidic chip as the driving force to promote the liquid flow into the enzyme inhibition reaction chamber and the detection chamber in turn to complete the enzyme inhibition reaction and activity analysis. The microfluidic chip-based enzyme inhibition reaction platform consists of upper and lower layers, the upper layer is a blank cover sheet, the lower layer is a microfluidic chip for immobilizing biochemical reagents, and the upper and lower layers are sealed to form fluid channels or chambers. The microfluidic chip is composed of enzyme inhibition reaction and analysis functional units; each enzyme inhibition reaction and analysis function unit is composed of a sample inlet, an enzyme inhibition reaction chamber, a detection chamber, and an air chamber. Using the enzyme inhibition reaction analysis method based on the microfluidic chip provided by the present invention, it is expected to realize on-site, rapid and accurate detection of toxic and harmful substances such as heavy metal ions and pesticide residues based on the enzyme inhibition reaction and analysis principle, and the market prospect is huge.

Description

Technical field [0001] The invention relates to the field of microfluidic chip analysis, in particular to a microfluidic-based enzyme inhibition reaction platform and an analysis method. Background technique [0002] Enzymes are important catalysts for maintaining the metabolism of organisms. Some components that enter the organism, such as heavy metal ions, pesticides, etc., have a particularly strong affinity with the active centers of certain enzymes. Enzyme inhibition reactions will cause enzymes to lose their activity and eventually induce organisms. The nervous system in the body undergoes a series of changes, which eventually leads to illness or death of the organism. Based on the above principles, enzyme inhibition reactions and analysis methods have extremely in-depth research and extremely wide applications in the detection of toxic and hazardous substances such as heavy metal ions, organophosphorus and carbamate pesticides. For example, at present, the rapid detection...

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Problems solved by technology

[0003] However, the existing rapid detection products for pesticide residues are often based on traditional biochemical analysis laboratory detection methods to achieve enzyme inhibition reaction analysis and detection

For example, the existing pesticide residue rapid detector mainly relies on a large number of accessories such as pipettes, beakers, cuvettes and analysis vessels to complete the enzyme inhibition analysis. substances), enzyme inhibition reaction, and color reaction process are cumbersome, all rely on manual completion, which takes a long time, and the operator needs to have certain professional knowledge, which can only be carried out in the laboratory, and it is difficult to achieve rapid on-site detection; in order to achieve rapid on-site detection People have also developed a quick test card for pesticide residues based on the principle of enzyme inhibition reaction. Although this method is simple and easy to implement, this method has disadvantages such as poor reaction uniformity and low analysis accuracy. Immobilize enzymes on the surface of porous solid media such as test paper, and then drop the solution sample on the surface of the test paper to complete the enzyme inhibition reaction process. It belongs to a "dry chemical method" of enzyme inhibition reaction analysis, which is affected by factors such as uneven fixation of reagents and solution evaporation. larger

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