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Paper-based micro-fluidic system and method for detection of pesticide residues

A technology of pesticide residue detection and microfluidic system, applied in the direction of electrochemical variables of materials, etc., can solve the problems of low degree of automation and cumbersome operation steps, and achieve the effect of improving the degree of automation, reducing costs and easy operation.

Active Publication Date: 2015-08-05
JIANGSU UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a fast, automatic, portable and low-cost pesticide residue detection technology for the common problems of cumbersome operation steps and low degree of automation in the pesticide residue detection methods based on enzyme inhibition method at home and abroad

Method used

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  • Paper-based micro-fluidic system and method for detection of pesticide residues

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Embodiment Construction

[0027] The specific embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings, taking the detection of organophosphorus pesticide residues in vegetables as an example.

[0028] In this embodiment, the paper microfluidic system for pesticide residue detection includes a paper microfluidic chip and a data acquisition device, wherein the structure of the paper microfluidic system is as follows figure 1 As shown, the hardware circuit composition of the data acquisition device is as follows figure 2 shown.

[0029] The specific detection process is:

[0030] Step 1: Put 5g of vegetable samples in a container such as a beaker, add 2000 μL of extractant to soak for a period of time, or drop the extractant on the surface of vegetables to obtain the extracted pesticide extract.

[0031] Step 2: Inject 2000 μL of the substrate acetylcholine solution from 1 injection pool A into the chip, and at the same time inject 2000 μL of ...

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Abstract

The invention discloses a paper-based micro-fluidic system and method for detection of pesticide residues. The paper-based micro-fluidic system is composed of a paper-based micro-fluidic chip, a three-electrode system and a data collection device, wherein a substrate and a pesticide extract liquid are injected into the paper-based micro-fluidic chip; a detection signal is transmitted to the data collection device through the three-electrode system; and the pesticide residue detection result is rapidly obtained on the basis of an enzyme inhibition principle. The paper-based micro-fluidic system is simple and convenient to operate; the automatic degree of an enzyme inhibition method detection process is improved; meanwhile, the cost is greatly reduced by use of a paper-based material; and the paper-based micro-fluidic system and the method can be applied to rapid and automatic field detection of the pesticide residues in samples such as fruits and vegetables, soil and water bodies.

Description

technical field [0001] The invention relates to the technical field of combining paper microfluidic chip analysis technology and pesticide residue detection technology, in particular to a paper microfluidic system and method for pesticide residue detection. Background technique [0002] Pesticides play an important role in agricultural production. In my country's agricultural production, there are a wide variety of pesticides used, including more than 600 types of insecticides, herbicides, fungicides, and rodenticides. Organophosphorus and carbamate pesticides are currently the most widely used pesticides in my country, and most of them are used on fruit and vegetable crops. Therefore, the most prominent pesticide residues in fruits, vegetables, aquatic products, soil and other agricultural products are organophosphorus pesticides and carbamate pesticides. For the monitoring and management of organophosphorus and carbamate pesticides, preventing agricultural produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 毛罕平施杰严玉婷杨宁左志强
Owner JIANGSU UNIV
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