Macadamia nut green peel extract, cosmetics employing same as active ingredient and preparation method of macadamia nut green peel extract
A green skin and nut technology, applied in the field of plant natural extracts and cosmetics, can solve the problems that have not yet been reported in cosmetics
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Embodiment 1
[0035] Experimental materials, instruments and reagents:
[0036] Material: Dried macadamia nut green skin, macadamia nut green skin fruit.
[0037] Instruments: ultrasonic extractor, 7200 spectrophotometer, centrifuge, water bath
[0038] Reagent samples: absolute ethanol, phosphate buffer, 1% NAOH, tyrosinase, tyrosine, DPPH, anthrone, concentrated sulfuric acid, glucose.
[0039] Experimental steps:
[0040] Extraction of polyphenols from macadamia nut green bark:
[0041] Take 10g of macadamia nut green husk powder, add 50ml of warm water to extract three times, a total of 150ml of water, then add 7.5ml of 5% sulfuric acid to the flask, cold soak for 24h, filter; then wash the filter cake with deionized water to remove residual sulfuric acid, until washing The liquid is neutral, and the polyphenols of the crudely extracted macadamia nut green bark are obtained.
[0042] Preparation of macadamia green bark water extract:
[0043] Preparation of ultrasonic water extract: ...
Embodiment 2
[0080] Extraction of polysaccharides from macadamia nut green bark:
[0081] Take by weighing 10g of dry macadamia nut green husk powder, which is tan and viscous, so as to obtain light tan macadamia nut green husk powder. Fresh macadamia nut green skin, after dehydration, obtains a pink viscous substance, and after drying at a low temperature of 40°C, its powder is a dark purple powder. Fresh macadamia nut green peels and dried macadamia nut green peels are extracted with polysaccharides, and the resulting polysaccharides, fresh macadamia nut green peels contain more gum than dried macadamia nut green peels, 10g macadamia nut green peels are extracted, dried macadamia nut green peels get 6.894g of polysaccharides, fresh macadamia nut green peels get 6.894g of polysaccharides, fresh macadamia nut green peels get Polysaccharide 4.743g. Fresh macadamia nut green skin has a higher water content, so the same weight of fresh macadamia nut green skin and dried macadamia nut green s...
Embodiment 3
[0108] Antioxidant activity of macadamia nut green bark polyphenols:
[0109] Macadamia nut green skin polyphenol Ac absorbance values measured by spectrophotometer: 0.542, 0.542, 0.541, average 0.542; Absorbance Ai and Aj absorbance values measured at 517nm are shown in Table 14; according to the formula: ⑦ get macadamia nut green skin water Antioxidant activity IP% of the extract is shown in Table 12; image 3 Antioxidant activity profile of polyphenols from macadamia nut green bark.
[0110] formula:
[0111] IP%=[1-(A i -A j ) / Ac]×100% ⑦
[0112] Table 11 Absorbance value of polyphenol antioxidant activity Ai, Aj of macadamia nut green bark
[0113]
[0114] Table 12 Antioxidant activity of polyphenols in macadamia nut green bark
[0115]
[0116] By measuring the antioxidant activity of macadamia nut green bark polyphenols at concentrations of 0.1, 0.2, 0.4, and 0.6 mg / ml, the obtained image 3 Mass concentration-IP% rate curve, the results show that maca...
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