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Radix gentianae extract and preparation method and application thereof

A kind of gentian extract and gentian technology are applied in the field of gentian extract and preparation thereof, and achieve the effect of simple preparation method and preventing skin aging

Inactive Publication Date: 2016-11-23
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of gentian as tyrosinase inhibitor, elastase inhibitor, antioxidant active agent and anti-aging active agent

Method used

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  • Radix gentianae extract and preparation method and application thereof
  • Radix gentianae extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Alpine Gentian Extract

[0027]Dry and pulverize 50g of Alpine Gentiana (Gentiana algida Pall.), mix and soak with 1000mL 70% ethanol solution (v / v, all ethanol solutions in the present invention are volume concentrations), ultrasonically assisted extraction for 1 hour, filter, and the filtrate is passed through After the solvent was removed under reduced pressure, 7.0 g of the crude extract of Gentiana alpine was obtained.

[0028] Get the crude extract of Gentiana alpine, dissolve it with deionized water, load it into the AB-8 resin-packed column, and use deionized water, 30% ethanol, 70% ethanol and 95% ethanol gradient elution successively (percentage is volume percent ), collect eluent respectively, remove ethanol and water under reduced pressure, weigh, obtain water elution part 4.7g, 30% ethanol elution part 0.6g, 70% ethanol elution part 1.0g and 95% ethanol elution part respectively 0.7g of alpine gentian extract.

Embodiment 2

[0030] Determination of total flavonoids in the extract of alpine gentian

[0031] The Alpine Gentiana extract prepared in Example 1 was used to determine the total flavonoid content in the Alpine Gentiana extract according to the method of the Pharmacopoeia of the People's Republic of China (2010 edition) with rutin as a standard.

[0032] Determination steps are: 1) Standard curve making: Accurately measure standard solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, add 30% ethanol to 6.0ml, add 5% sodium nitrite Solution (mass percentage concentration) 1ml. Mix well, stand for 6 minutes, add 1ml of 10% aluminum nitrate solution (mass percentage concentration), shake well, stand for 6 minutes. Add 10ml of sodium hydroxide test solution, add 30% ethanol to the scale, shake well, let stand for 15 minutes, use the corresponding reagent as a blank, measure the absorbance at a wavelength of 510nm by UV-visible spectrophotometry, and take the absorb...

Embodiment 3

[0038] Detection of tyrosinase inhibitory activity

[0039] The Gentiana alpine extract of each elution position prepared in Example 1 was completely dissolved in deionized water, and was formulated into a 1.0 mg / mL aqueous solution, i.e. a sample solution, for measuring tyrosinase inhibitory activity, with arbutin as reference.

[0040] The measurement steps are: mix 70 μL of phosphate buffer solution (pH6.8, 30 mM), 80 μL of 1.66 mM tyrosine solution, and 80 μL of sample solution, incubate in a constant temperature water tank at 37°C for more than 5 minutes, add tyrosinase solution (125 U / ml) 10 μL, after constant temperature at 37°C for 10 minutes, measure the absorbance A at 475nm wavelength with a microplate reader 475 . The sample aqueous solution was replaced with deionized water, and the absorbance was also measured as a reference solution, and the results are shown in Table 2.

[0041] The formula for calculating the inhibition rate of tyrosinase activity is: inhi...

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Abstract

The invention discloses a radix gentianae extract and a preparation method and application thereof. The preparation method of the radix gentianae extract comprises the following steps that 1, radix gentianae and a solvent are mixed, extracted and filtered, pressure is reduced, the solvent is removed, and the radix gentianae extract is obtained; 2, the radix gentianae extract is dissolved in water, sampled to a resin filling column andeluted through water and an ethanol solution in sequence, eluent is collected, pressure is reduced, ethyl alcohol and water are removed, and the radix gentianae extract is obtained. The preparation method of the radix gentianae extract is simple, and the obtained radix gentianae extract can be applied as tyrosinase inhibitor, elastinase inhibitor, antioxidant active agent and anti-aging active agent, preferentially applied in preparation of skin care products or cosmetics.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a gentian extract, a preparation method and application thereof. Background technique [0002] In 1956, Harman proposed the theory of free radical aging [Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11: 289-300.], which believed that excessive free radicals were the cause of biological aging important reason. According to this theory, excess active oxygen free radicals in the body react with unsaturated fatty acids to produce substances such as malondialdehyde, and malondialdehyde will react with proteins on the cell membrane to produce brown pigment, which precipitates on the skin and becomes various stains. Excessive free radicals can also cause cross-linking and denaturation of collagen fibers and elastic fibers in the skin, making them brittle and losing elasticity. When the skin is insufficiently hydrated, it is easy to break t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08
Inventor 方兆华沈忠海李慧黄晓青
Owner JALA GROUP CORPORATION
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