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The invention discloses a preparation method and a weeding application of a cis-p-alkyl-1,8-disecondary amine compound. The method comprises the following steps: using cis-p-alkyl-1,8-diSchiff base compound as a raw material, adding a educing agent to a polar organic solvent in batches at a temperature of -10-50 DEG C, carrying out a reaction for 1-24 h, quenching the obtained reaction solution with distilled water after the reaction is finished, performing extraction with dichloromethane, drying the obtained extract with anhydrous sodium sulfate, filtering the obtained extract, carrying out reduced pressure distillation to obtain the cis-p-alkyl-1,8-disecondary amine compound, and performing recrystallization or silica gel column chromatography to obtain the pure product. The cis-p-alkyl-1,8-disecondary amine compound is detected by a culture dish seed germination technology, and the weeding activity of the compound is evaluated according to the growth injury symptoms of roots and stems of rice barnyard grass. The method of the invention has the advantages of simple process, mild conditions, high rate, high yield, and wide applicability of the substrate, and the product cis-p-alkyl-1,8-disecondary amine compound has an excellent inhibition effect on the growth of the roots and stems of the rice barnyard grass.